Picrosirius Red and Polarization Microscopy – A Tool for Gender Differentiation
Introduction: Forensic dentistry is a branch of dentistry which in collaboration with legal profession serves an important role to maintain justice system of a country. Forensic dentists play a major role in identification of an individual. Within the literature various methods have been found t...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2017-01-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/9296/22863_CE[Ra1]_F(DK)_PF1(NE_DK)_PFA(DK)_PF2(SH_DK).pdf |
Summary: | Introduction: Forensic dentistry is a branch of dentistry which
in collaboration with legal profession serves an important
role to maintain justice system of a country. Forensic dentists
play a major role in identification of an individual. Within the
literature various methods have been found to be useful in
gender differentiation. An attempt was made for differentiation
of gender using picrosirius red and polarization microscopy.
Aim: To evaluate picrosirius red and polarization microscopy
as a tool for gender differentiation by observing birefringence
pattern and distribution of thick and thin collagen fibers in
males and females.
Materials and Methods: Labial mucosal tissue obtained from
30 deceased individuals (18 male and 12 female) during autopsy
was fixed in 10% formalin at 12th hour. Tissue was processed,
sectioned and stained using picrosirius red stain and the
birefringence pattern of collagen fibers were studied with
polarization microscope. The results were statistically analyzed
using Z-test and one-way ANOVA to draw the significance.
Results: The proportion of thick and thin fibres among males
and females were compared. It was found that there was
statistically significant difference in proportion of thick and thin
fibers between male and female. Thick fibres in males were
(78.13%) more than females (65.74%) and thin fibres were more
in females (34.24%) than males (21.32%).
Conclusion: Picrosirius red and polarization microscopy may
be used as a tool for gender differentiation. Yet the manner of
death has to be considered during gender differentiation using
this method, as in the present study out of 30 cases studied
three cases of death due to debilitating diseases and poison
consumption showed altered collagen distribution. |
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ISSN: | 2249-782X 0973-709X |