Picrosirius Red and Polarization Microscopy – A Tool for Gender Differentiation
Introduction: Forensic dentistry is a branch of dentistry which in collaboration with legal profession serves an important role to maintain justice system of a country. Forensic dentists play a major role in identification of an individual. Within the literature various methods have been found t...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2017-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9296/22863_CE[Ra1]_F(DK)_PF1(NE_DK)_PFA(DK)_PF2(SH_DK).pdf |
| _version_ | 1811208295878230016 |
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| author | BK Charan Gowda Ganganna Kokila Pillai Arun Gopinathan Kunigal Shivaprakash Praveen |
| author_facet | BK Charan Gowda Ganganna Kokila Pillai Arun Gopinathan Kunigal Shivaprakash Praveen |
| author_sort | BK Charan Gowda |
| collection | DOAJ |
| description | Introduction: Forensic dentistry is a branch of dentistry which
in collaboration with legal profession serves an important
role to maintain justice system of a country. Forensic dentists
play a major role in identification of an individual. Within the
literature various methods have been found to be useful in
gender differentiation. An attempt was made for differentiation
of gender using picrosirius red and polarization microscopy.
Aim: To evaluate picrosirius red and polarization microscopy
as a tool for gender differentiation by observing birefringence
pattern and distribution of thick and thin collagen fibers in
males and females.
Materials and Methods: Labial mucosal tissue obtained from
30 deceased individuals (18 male and 12 female) during autopsy
was fixed in 10% formalin at 12th hour. Tissue was processed,
sectioned and stained using picrosirius red stain and the
birefringence pattern of collagen fibers were studied with
polarization microscope. The results were statistically analyzed
using Z-test and one-way ANOVA to draw the significance.
Results: The proportion of thick and thin fibres among males
and females were compared. It was found that there was
statistically significant difference in proportion of thick and thin
fibers between male and female. Thick fibres in males were
(78.13%) more than females (65.74%) and thin fibres were more
in females (34.24%) than males (21.32%).
Conclusion: Picrosirius red and polarization microscopy may
be used as a tool for gender differentiation. Yet the manner of
death has to be considered during gender differentiation using
this method, as in the present study out of 30 cases studied
three cases of death due to debilitating diseases and poison
consumption showed altered collagen distribution. |
| first_indexed | 2024-04-12T04:19:24Z |
| format | Article |
| id | doaj.art-e238174638f843129dadc7ea4adbd1a2 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-12T04:19:24Z |
| publishDate | 2017-01-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-e238174638f843129dadc7ea4adbd1a22022-12-22T03:48:18ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-01-01111ZC107ZC10910.7860/JCDR/2017/22863.9296Picrosirius Red and Polarization Microscopy – A Tool for Gender Differentiation BK Charan Gowda0Ganganna Kokila1Pillai Arun Gopinathan2Kunigal Shivaprakash Praveen3Reader, Department of Oral Pathology, Sri Siddhartha Dental College, Sri Siddhartha Academy of Higher Education, Tumakur, Karnataka, India.Professor, Department of Oral Pathology, Sri Siddhartha Dental College, Sri Siddhartha Academy of Higher Education, Tumakur, Karnataka, India.Senior Lecturer, Department of Oral Pathology, Sri Sankara Dental College, Kollam, Kerala, India.Senior Lecturer, Department of Oral Pathology, Sri Siddhartha Dental College, Sri Siddhartha Academy of Higher Education, Tumakur, Karnataka, India.Introduction: Forensic dentistry is a branch of dentistry which in collaboration with legal profession serves an important role to maintain justice system of a country. Forensic dentists play a major role in identification of an individual. Within the literature various methods have been found to be useful in gender differentiation. An attempt was made for differentiation of gender using picrosirius red and polarization microscopy. Aim: To evaluate picrosirius red and polarization microscopy as a tool for gender differentiation by observing birefringence pattern and distribution of thick and thin collagen fibers in males and females. Materials and Methods: Labial mucosal tissue obtained from 30 deceased individuals (18 male and 12 female) during autopsy was fixed in 10% formalin at 12th hour. Tissue was processed, sectioned and stained using picrosirius red stain and the birefringence pattern of collagen fibers were studied with polarization microscope. The results were statistically analyzed using Z-test and one-way ANOVA to draw the significance. Results: The proportion of thick and thin fibres among males and females were compared. It was found that there was statistically significant difference in proportion of thick and thin fibers between male and female. Thick fibres in males were (78.13%) more than females (65.74%) and thin fibres were more in females (34.24%) than males (21.32%). Conclusion: Picrosirius red and polarization microscopy may be used as a tool for gender differentiation. Yet the manner of death has to be considered during gender differentiation using this method, as in the present study out of 30 cases studied three cases of death due to debilitating diseases and poison consumption showed altered collagen distribution.https://jcdr.net/articles/PDF/9296/22863_CE[Ra1]_F(DK)_PF1(NE_DK)_PFA(DK)_PF2(SH_DK).pdfbirefringencecollagen fibersforensic dentistry |
| spellingShingle | BK Charan Gowda Ganganna Kokila Pillai Arun Gopinathan Kunigal Shivaprakash Praveen Picrosirius Red and Polarization Microscopy – A Tool for Gender Differentiation Journal of Clinical and Diagnostic Research birefringence collagen fibers forensic dentistry |
| title | Picrosirius Red and Polarization Microscopy – A Tool for Gender Differentiation |
| title_full | Picrosirius Red and Polarization Microscopy – A Tool for Gender Differentiation |
| title_fullStr | Picrosirius Red and Polarization Microscopy – A Tool for Gender Differentiation |
| title_full_unstemmed | Picrosirius Red and Polarization Microscopy – A Tool for Gender Differentiation |
| title_short | Picrosirius Red and Polarization Microscopy – A Tool for Gender Differentiation |
| title_sort | picrosirius red and polarization microscopy a tool for gender differentiation |
| topic | birefringence collagen fibers forensic dentistry |
| url | https://jcdr.net/articles/PDF/9296/22863_CE[Ra1]_F(DK)_PF1(NE_DK)_PFA(DK)_PF2(SH_DK).pdf |
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