The first dog-origin porcine circovirus type 4 complete genomic sequence have high homology with that of pig-derived strains
Introduction: Porcine circovirus 4 (PCV4) was discovered in 2019 and then proved to be pathogenic to piglets. Nevertheless, few studies were currently available about PCV4 infection in species other than pigs and there is no information about the prevalence of PCV4 in dogs.Methods: To fill this gap,...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1121177/full |
| _version_ | 1797895441615945728 |
|---|---|
| author | Tong Xu Lan Chen Bing-Zhou Huang Ling Zhu Ling Zhu Xian-Gang Sun Si-Yuan Lai Yan-Ru Ai Yuan-Cheng Zhou Yuan-Cheng Zhou Zhi-Wen Xu Zhi-Wen Xu |
| author_facet | Tong Xu Lan Chen Bing-Zhou Huang Ling Zhu Ling Zhu Xian-Gang Sun Si-Yuan Lai Yan-Ru Ai Yuan-Cheng Zhou Yuan-Cheng Zhou Zhi-Wen Xu Zhi-Wen Xu |
| author_sort | Tong Xu |
| collection | DOAJ |
| description | Introduction: Porcine circovirus 4 (PCV4) was discovered in 2019 and then proved to be pathogenic to piglets. Nevertheless, few studies were currently available about PCV4 infection in species other than pigs and there is no information about the prevalence of PCV4 in dogs.Methods: To fill this gap, 264 dog samples were collected from animal hospitals in the Southwest of China from 2021 to 2022 and screened for PCV4. Moreover, the complete genome of one PCV4 strain (SCABTC-Dog2022) were obtained successfully and shared a high identity (97.9-99.0%) with other PCV4 strains derived from pigs, dairy cows, raccoon dogs and foxes. The SCABTC-Dog2022 were analyzed together with 51 reference sequences.Results and Discussion: The detected results showed a low percentage of PCV-4 DNA (1.14%, 3/264), indicating that PCV4 could be identified in dogs in southwest China. Phylogenetic tree showed that SCABTC-Dog2022 strain derived from dog were clustered in a closed relative and geographically coherent branch with other PCV4 strains collected from four provinces (Sichuan, Fujian, Hunan and Inner Mongolia) of China. To our knowledge, it is the first detection of PCV4 in dogs globally. The association between PCV4 status and clinical syndromes in dogs deserves additional investigations. |
| first_indexed | 2024-04-10T07:26:58Z |
| format | Article |
| id | doaj.art-e2395f74b19b425ca818c8b6f3777c9f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T07:26:58Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e2395f74b19b425ca818c8b6f3777c9f2023-02-24T07:05:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.11211771121177The first dog-origin porcine circovirus type 4 complete genomic sequence have high homology with that of pig-derived strainsTong Xu0Lan Chen1Bing-Zhou Huang2Ling Zhu3Ling Zhu4Xian-Gang Sun5Si-Yuan Lai6Yan-Ru Ai7Yuan-Cheng Zhou8Yuan-Cheng Zhou9Zhi-Wen Xu10Zhi-Wen Xu11College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaSichuan Key Laboratory of Animal Epidemic Disease and Human Health, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, ChinaLivestock and Poultry Biological Products Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaSichuan Key Laboratory of Animal Epidemic Disease and Human Health, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaIntroduction: Porcine circovirus 4 (PCV4) was discovered in 2019 and then proved to be pathogenic to piglets. Nevertheless, few studies were currently available about PCV4 infection in species other than pigs and there is no information about the prevalence of PCV4 in dogs.Methods: To fill this gap, 264 dog samples were collected from animal hospitals in the Southwest of China from 2021 to 2022 and screened for PCV4. Moreover, the complete genome of one PCV4 strain (SCABTC-Dog2022) were obtained successfully and shared a high identity (97.9-99.0%) with other PCV4 strains derived from pigs, dairy cows, raccoon dogs and foxes. The SCABTC-Dog2022 were analyzed together with 51 reference sequences.Results and Discussion: The detected results showed a low percentage of PCV-4 DNA (1.14%, 3/264), indicating that PCV4 could be identified in dogs in southwest China. Phylogenetic tree showed that SCABTC-Dog2022 strain derived from dog were clustered in a closed relative and geographically coherent branch with other PCV4 strains collected from four provinces (Sichuan, Fujian, Hunan and Inner Mongolia) of China. To our knowledge, it is the first detection of PCV4 in dogs globally. The association between PCV4 status and clinical syndromes in dogs deserves additional investigations.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1121177/fullporcine circovirus 4dogmolecular detectiongenetic analysiscross-species transmission |
| spellingShingle | Tong Xu Lan Chen Bing-Zhou Huang Ling Zhu Ling Zhu Xian-Gang Sun Si-Yuan Lai Yan-Ru Ai Yuan-Cheng Zhou Yuan-Cheng Zhou Zhi-Wen Xu Zhi-Wen Xu The first dog-origin porcine circovirus type 4 complete genomic sequence have high homology with that of pig-derived strains Frontiers in Microbiology porcine circovirus 4 dog molecular detection genetic analysis cross-species transmission |
| title | The first dog-origin porcine circovirus type 4 complete genomic sequence have high homology with that of pig-derived strains |
| title_full | The first dog-origin porcine circovirus type 4 complete genomic sequence have high homology with that of pig-derived strains |
| title_fullStr | The first dog-origin porcine circovirus type 4 complete genomic sequence have high homology with that of pig-derived strains |
| title_full_unstemmed | The first dog-origin porcine circovirus type 4 complete genomic sequence have high homology with that of pig-derived strains |
| title_short | The first dog-origin porcine circovirus type 4 complete genomic sequence have high homology with that of pig-derived strains |
| title_sort | first dog origin porcine circovirus type 4 complete genomic sequence have high homology with that of pig derived strains |
| topic | porcine circovirus 4 dog molecular detection genetic analysis cross-species transmission |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1121177/full |
| work_keys_str_mv | AT tongxu thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT lanchen thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT bingzhouhuang thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT lingzhu thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT lingzhu thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT xiangangsun thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT siyuanlai thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT yanruai thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT yuanchengzhou thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT yuanchengzhou thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT zhiwenxu thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT zhiwenxu thefirstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT tongxu firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT lanchen firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT bingzhouhuang firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT lingzhu firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT lingzhu firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT xiangangsun firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT siyuanlai firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT yanruai firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT yuanchengzhou firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT yuanchengzhou firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT zhiwenxu firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains AT zhiwenxu firstdogoriginporcinecircovirustype4completegenomicsequencehavehighhomologywiththatofpigderivedstrains |