Isolation and Characterization of a Deoxynivalenol-Degrading Bacterium <i>Bacillus licheniformis YB9</i> with the Capability of Modulating Intestinal Microbial Flora of Mice
Deoxynivalenol (DON) is one of the most prevalent food- and feed-associated mycotoxins. It frequently contaminates agricultural commodities and poses serious threats to human and animal health and leads to tremendous economic losses globally. Much attention has been paid to using microorganisms to d...
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MDPI AG
2020-03-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/3/184 |
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| author | Shiwei Wang Qiuqiu Hou Qianqian Guo Jian Zhang Yanmei Sun Hong Wei Lixin Shen |
| author_facet | Shiwei Wang Qiuqiu Hou Qianqian Guo Jian Zhang Yanmei Sun Hong Wei Lixin Shen |
| author_sort | Shiwei Wang |
| collection | DOAJ |
| description | Deoxynivalenol (DON) is one of the most prevalent food- and feed-associated mycotoxins. It frequently contaminates agricultural commodities and poses serious threats to human and animal health and leads to tremendous economic losses globally. Much attention has been paid to using microorganisms to detoxify DON. In this study, a <i>Bacillus licheniformis</i> strain named YB9 with a strong ability to detoxify DON was isolated and characterized from a moldy soil sample. YB9 could degrade more than 82.67% of 1 mg/L DON within 48 h at 37 °C and showed strong survival and DON degradation rate at simulated gastric fluid. The effects of YB9 on mice with DON intragastrical administration were further investigated by biochemical and histopathological examination and the gut microbiota was analyzed by 16S rRNA Illumina sequencing technology. The results showed that DON increased the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatinine (Cr), decreased those of immunoglobulin G (IgG) and IgM in serum, and resulted in severe pathological damage of the liver, kidney, and spleen. By contrast, YB9 supplementation obviously inhibited or attenuated the damages caused by DON in mice. In addition, YB9 addition repaired the DON-induced dysbiosis of intestinal flora, characterized by recovering the balance of Firmicutes and Bacteroidetes to the normal level and decreasing the abundance of the potentially harmful bacterium <i>Turicibacter</i> and the excessive <i>Lactobacillus</i> caused by DON. Taken together, DON-degrading strain YB9 might be used as potential probiotic additive for improving food and feed safety and modulating the intestinal microbial flora of humans and animals. |
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| last_indexed | 2024-04-14T01:21:06Z |
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| spelling | doaj.art-e239f84f36d04712acc45d0b9e7145282022-12-22T02:20:38ZengMDPI AGToxins2072-66512020-03-0112318410.3390/toxins12030184toxins12030184Isolation and Characterization of a Deoxynivalenol-Degrading Bacterium <i>Bacillus licheniformis YB9</i> with the Capability of Modulating Intestinal Microbial Flora of MiceShiwei Wang0Qiuqiu Hou1Qianqian Guo2Jian Zhang3Yanmei Sun4Hong Wei5Lixin Shen6Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, ChinaDeoxynivalenol (DON) is one of the most prevalent food- and feed-associated mycotoxins. It frequently contaminates agricultural commodities and poses serious threats to human and animal health and leads to tremendous economic losses globally. Much attention has been paid to using microorganisms to detoxify DON. In this study, a <i>Bacillus licheniformis</i> strain named YB9 with a strong ability to detoxify DON was isolated and characterized from a moldy soil sample. YB9 could degrade more than 82.67% of 1 mg/L DON within 48 h at 37 °C and showed strong survival and DON degradation rate at simulated gastric fluid. The effects of YB9 on mice with DON intragastrical administration were further investigated by biochemical and histopathological examination and the gut microbiota was analyzed by 16S rRNA Illumina sequencing technology. The results showed that DON increased the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatinine (Cr), decreased those of immunoglobulin G (IgG) and IgM in serum, and resulted in severe pathological damage of the liver, kidney, and spleen. By contrast, YB9 supplementation obviously inhibited or attenuated the damages caused by DON in mice. In addition, YB9 addition repaired the DON-induced dysbiosis of intestinal flora, characterized by recovering the balance of Firmicutes and Bacteroidetes to the normal level and decreasing the abundance of the potentially harmful bacterium <i>Turicibacter</i> and the excessive <i>Lactobacillus</i> caused by DON. Taken together, DON-degrading strain YB9 might be used as potential probiotic additive for improving food and feed safety and modulating the intestinal microbial flora of humans and animals.https://www.mdpi.com/2072-6651/12/3/184deoxynivalenoldegradation and detoxification<i>bacillus licheniformis</i> yb9intestinal microbial flora |
| spellingShingle | Shiwei Wang Qiuqiu Hou Qianqian Guo Jian Zhang Yanmei Sun Hong Wei Lixin Shen Isolation and Characterization of a Deoxynivalenol-Degrading Bacterium <i>Bacillus licheniformis YB9</i> with the Capability of Modulating Intestinal Microbial Flora of Mice Toxins deoxynivalenol degradation and detoxification <i>bacillus licheniformis</i> yb9 intestinal microbial flora |
| title | Isolation and Characterization of a Deoxynivalenol-Degrading Bacterium <i>Bacillus licheniformis YB9</i> with the Capability of Modulating Intestinal Microbial Flora of Mice |
| title_full | Isolation and Characterization of a Deoxynivalenol-Degrading Bacterium <i>Bacillus licheniformis YB9</i> with the Capability of Modulating Intestinal Microbial Flora of Mice |
| title_fullStr | Isolation and Characterization of a Deoxynivalenol-Degrading Bacterium <i>Bacillus licheniformis YB9</i> with the Capability of Modulating Intestinal Microbial Flora of Mice |
| title_full_unstemmed | Isolation and Characterization of a Deoxynivalenol-Degrading Bacterium <i>Bacillus licheniformis YB9</i> with the Capability of Modulating Intestinal Microbial Flora of Mice |
| title_short | Isolation and Characterization of a Deoxynivalenol-Degrading Bacterium <i>Bacillus licheniformis YB9</i> with the Capability of Modulating Intestinal Microbial Flora of Mice |
| title_sort | isolation and characterization of a deoxynivalenol degrading bacterium i bacillus licheniformis yb9 i with the capability of modulating intestinal microbial flora of mice |
| topic | deoxynivalenol degradation and detoxification <i>bacillus licheniformis</i> yb9 intestinal microbial flora |
| url | https://www.mdpi.com/2072-6651/12/3/184 |
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