Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells
Periodontitis is an inflammatory disease that leads to periodontal tissue destruction and bone resorption. Proliferation and differentiation of cells capable of differentiating into osteoblasts is important for reconstructing periodontal tissues destroyed by periodontitis. In this study, the effects...
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MDPI AG
2021-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/11/1542 |
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| author | Byul-Bora Choi Jeong-Hae Choi Tae-Hyung Kang Seok-Jun Lee Gyoo-Cheon Kim |
| author_facet | Byul-Bora Choi Jeong-Hae Choi Tae-Hyung Kang Seok-Jun Lee Gyoo-Cheon Kim |
| author_sort | Byul-Bora Choi |
| collection | DOAJ |
| description | Periodontitis is an inflammatory disease that leads to periodontal tissue destruction and bone resorption. Proliferation and differentiation of cells capable of differentiating into osteoblasts is important for reconstructing periodontal tissues destroyed by periodontitis. In this study, the effects of the nozone (no-ozone) cold plasma (NCP) treatment on osteoblastic differentiation in periodontal ligament (PDL) cells were investigated. To test the toxicity of NCP on PDL cells, various NCP treatment methods and durations were tested, and time-dependent cell proliferation was analyzed using a water-soluble tetrazolium salts-1 assay. To determine the effect of NCP on PDL cell differentiation, the cells were provided with osteogenic media immediately after an NCP treatment to induce differentiation; the cells were then analyzed using alkaline phosphatase (ALP) staining, an ALP activity assay, real time PCR, and Alizarin Red S staining. The NCP treatment without toxicity on PDL cells was the condition of 1-min NCP treatment immediately followed by the replacement with fresh media. NCP increased ALP, osteocalcin, osteonectin, and osteopontin expression, as well as mineralization nodule formation. NCP treatment promotes osteoblastic differentiation of PDL cells; therefore, it may be beneficial for treating periodontitis. |
| first_indexed | 2024-03-10T05:41:44Z |
| format | Article |
| id | doaj.art-e23bcb0eff1e43fa88fb56ad317568bc |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T05:41:44Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj.art-e23bcb0eff1e43fa88fb56ad317568bc2023-11-22T22:29:46ZengMDPI AGBiomedicines2227-90592021-10-01911154210.3390/biomedicines9111542Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament CellsByul-Bora Choi0Jeong-Hae Choi1Tae-Hyung Kang2Seok-Jun Lee3Gyoo-Cheon Kim4Corporate Affiliated Research Institute, Feagle Co., Ltd., Yangsan 50614, KoreaCorporate Affiliated Research Institute, Feagle Co., Ltd., Yangsan 50614, KoreaOnnuri Pain Clinic, Busan 48585, KoreaAssemble Plastic Surgery, Seoul 06526, KoreaDepartment of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan 50612, KoreaPeriodontitis is an inflammatory disease that leads to periodontal tissue destruction and bone resorption. Proliferation and differentiation of cells capable of differentiating into osteoblasts is important for reconstructing periodontal tissues destroyed by periodontitis. In this study, the effects of the nozone (no-ozone) cold plasma (NCP) treatment on osteoblastic differentiation in periodontal ligament (PDL) cells were investigated. To test the toxicity of NCP on PDL cells, various NCP treatment methods and durations were tested, and time-dependent cell proliferation was analyzed using a water-soluble tetrazolium salts-1 assay. To determine the effect of NCP on PDL cell differentiation, the cells were provided with osteogenic media immediately after an NCP treatment to induce differentiation; the cells were then analyzed using alkaline phosphatase (ALP) staining, an ALP activity assay, real time PCR, and Alizarin Red S staining. The NCP treatment without toxicity on PDL cells was the condition of 1-min NCP treatment immediately followed by the replacement with fresh media. NCP increased ALP, osteocalcin, osteonectin, and osteopontin expression, as well as mineralization nodule formation. NCP treatment promotes osteoblastic differentiation of PDL cells; therefore, it may be beneficial for treating periodontitis.https://www.mdpi.com/2227-9059/9/11/1542differentiationno-ozone cold plasmaosteoblastperiodontal ligament cells |
| spellingShingle | Byul-Bora Choi Jeong-Hae Choi Tae-Hyung Kang Seok-Jun Lee Gyoo-Cheon Kim Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells Biomedicines differentiation no-ozone cold plasma osteoblast periodontal ligament cells |
| title | Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells |
| title_full | Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells |
| title_fullStr | Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells |
| title_full_unstemmed | Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells |
| title_short | Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells |
| title_sort | enhancement of osteoblast differentiation using no ozone cold plasma on human periodontal ligament cells |
| topic | differentiation no-ozone cold plasma osteoblast periodontal ligament cells |
| url | https://www.mdpi.com/2227-9059/9/11/1542 |
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