Acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with Pantoea agglomerans and Microbacterium barkeri
Case description Five workers (2 males and 3 females) employed in a furniture factory located in eastern Poland developed hypersensitivity pneumonitis (HP) after the pine wood used for furniture production was replaced by birch wood. All of them reported onset of respiratory and general symptoms (co...
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Institute of Rural Health
2019-12-01
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| Series: | Annals of Agricultural and Environmental Medicine |
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| Online Access: | http://www.journalssystem.com/aaem/Acute-hypersensitivity-pneumonitis-in-woodworkers-caused-by-the-inhalation-of-birch,114931,0,2.html |
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| author | Barbara Mackiewicz Jacek Dutkiewicz Jan Siwiec Tomasz Kucharczyk Elżbieta Siek Angelina Wójcik-Fatla Grażyna Cholewa Alicja Cholewa Mariola Paściak Krzysztof Pawlik Bogumiła Szponar Janusz Milanowski |
| author_facet | Barbara Mackiewicz Jacek Dutkiewicz Jan Siwiec Tomasz Kucharczyk Elżbieta Siek Angelina Wójcik-Fatla Grażyna Cholewa Alicja Cholewa Mariola Paściak Krzysztof Pawlik Bogumiła Szponar Janusz Milanowski |
| author_sort | Barbara Mackiewicz |
| collection | DOAJ |
| description | Case description
Five workers (2 males and 3 females) employed in a furniture factory located in eastern Poland developed hypersensitivity pneumonitis (HP) after the pine wood used for furniture production was replaced by birch wood. All of them reported onset of respiratory and general symptoms (cough, shortness of breath, general malaise) after inhalation exposure to birch dust, showed crackles at auscultation, ground-glass attenuations in HRCT examination, and lymphocytosis in the BAL examination. The diagnosis of acute HP was set in 4 persons and the diagnosis of subacute HP in one.
Identification of specific allergen
Samples of birch wood associated with evoking disease symptoms were subjected to microbiological analysis with the conventional and molecular methods. Two bacterial isolates were found to occur in large quantities (of the order 10 8 CFU/g) in examined samples: Gram-negative bacterium of the species Pantoea agglomerans and a non-filamentous Gram-positive actinobacterium of the species Microbacterium barkeri . In the test for inhibition of leukocyte migration, 4 out of 5 examined patients showed a positive reaction in the presence of P. agglomerans and 2 in the presence of M. barkeri . Only one person showed the presence of precipitins to P. agglomerans and none to M. barkeri . In the inhalation challenge, which is the most relevant allergological test in the HP diagnostics, all patients reacted positively to P. agglomerans and only one to M. barkeri . The results indicate that P. agglomerans developing in birch wood was the main agent causing HP in the workers exposed to the inhalation of dust from this wood, while the etiologic role of M. barkeri is probably secondary.
Conclusion
The results demonstrate that apart from fungi and filamentous actinobacteria, regarded until recently as causative agents of HP in woodworkers, Gram-negative bacteria and non-filamentous actinobacteria may also elicit disease symptoms in the workers processing wood infected with large amounts of these microorganisms. The results obtained also seem to indicate that cellular-mediated reactions are more significant for causing disease symptoms compared to those that are precipitin-mediated. |
| first_indexed | 2024-12-10T10:10:53Z |
| format | Article |
| id | doaj.art-e2432e368d284172ac64dd146a0b75f0 |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-10T10:10:53Z |
| publishDate | 2019-12-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-e2432e368d284172ac64dd146a0b75f02022-12-22T01:53:08ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632019-12-0126464465510.26444/aaem/114931114931Acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with Pantoea agglomerans and Microbacterium barkeriBarbara Mackiewicz0Jacek Dutkiewicz1Jan Siwiec2Tomasz Kucharczyk3Elżbieta Siek4Angelina Wójcik-Fatla5Grażyna Cholewa6Alicja Cholewa7Mariola Paściak8Krzysztof Pawlik9Bogumiła Szponar10Janusz Milanowski11Department of Pneumonology, Oncology and Allergology, Medical University, Lublin, PolandDepartment of Biological Health Hazards and Parasitology, Institute of Rural Health, Lublin, PolandDepartment of Pneumonology, Oncology and Allergology, Medical University, Lublin, PolandDepartment of Pneumonology, Oncology and Allergology, Medical University, Lublin, PolandDepartment of Medical Radiology, Medical University, Lublin, PolandDepartment of Biological Health Hazards and Parasitology, Institute of Rural Health, Lublin, PolandDepartment of Biological Health Hazards and Parasitology, Institute of Rural Health, Lublin, PolandDepartment of Biological Health Hazards and Parasitology, Institute of Rural Health, Lublin, PolandDepartment of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, PolandDepartment of Microbiology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, PolandDepartment of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, PolandDepartment of Pneumonology, Oncology and Allergology, Medical University, Lublin, PolandCase description Five workers (2 males and 3 females) employed in a furniture factory located in eastern Poland developed hypersensitivity pneumonitis (HP) after the pine wood used for furniture production was replaced by birch wood. All of them reported onset of respiratory and general symptoms (cough, shortness of breath, general malaise) after inhalation exposure to birch dust, showed crackles at auscultation, ground-glass attenuations in HRCT examination, and lymphocytosis in the BAL examination. The diagnosis of acute HP was set in 4 persons and the diagnosis of subacute HP in one. Identification of specific allergen Samples of birch wood associated with evoking disease symptoms were subjected to microbiological analysis with the conventional and molecular methods. Two bacterial isolates were found to occur in large quantities (of the order 10 8 CFU/g) in examined samples: Gram-negative bacterium of the species Pantoea agglomerans and a non-filamentous Gram-positive actinobacterium of the species Microbacterium barkeri . In the test for inhibition of leukocyte migration, 4 out of 5 examined patients showed a positive reaction in the presence of P. agglomerans and 2 in the presence of M. barkeri . Only one person showed the presence of precipitins to P. agglomerans and none to M. barkeri . In the inhalation challenge, which is the most relevant allergological test in the HP diagnostics, all patients reacted positively to P. agglomerans and only one to M. barkeri . The results indicate that P. agglomerans developing in birch wood was the main agent causing HP in the workers exposed to the inhalation of dust from this wood, while the etiologic role of M. barkeri is probably secondary. Conclusion The results demonstrate that apart from fungi and filamentous actinobacteria, regarded until recently as causative agents of HP in woodworkers, Gram-negative bacteria and non-filamentous actinobacteria may also elicit disease symptoms in the workers processing wood infected with large amounts of these microorganisms. The results obtained also seem to indicate that cellular-mediated reactions are more significant for causing disease symptoms compared to those that are precipitin-mediated.http://www.journalssystem.com/aaem/Acute-hypersensitivity-pneumonitis-in-woodworkers-caused-by-the-inhalation-of-birch,114931,0,2.htmlhypersensitivity pneumonitiswood dustbacteriapantoea agglomeransmicrobacterium barkeriinhalation challengeoccupational exposure |
| spellingShingle | Barbara Mackiewicz Jacek Dutkiewicz Jan Siwiec Tomasz Kucharczyk Elżbieta Siek Angelina Wójcik-Fatla Grażyna Cholewa Alicja Cholewa Mariola Paściak Krzysztof Pawlik Bogumiła Szponar Janusz Milanowski Acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with Pantoea agglomerans and Microbacterium barkeri Annals of Agricultural and Environmental Medicine hypersensitivity pneumonitis wood dust bacteria pantoea agglomerans microbacterium barkeri inhalation challenge occupational exposure |
| title | Acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with Pantoea agglomerans and Microbacterium barkeri |
| title_full | Acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with Pantoea agglomerans and Microbacterium barkeri |
| title_fullStr | Acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with Pantoea agglomerans and Microbacterium barkeri |
| title_full_unstemmed | Acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with Pantoea agglomerans and Microbacterium barkeri |
| title_short | Acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with Pantoea agglomerans and Microbacterium barkeri |
| title_sort | acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with pantoea agglomerans and microbacterium barkeri |
| topic | hypersensitivity pneumonitis wood dust bacteria pantoea agglomerans microbacterium barkeri inhalation challenge occupational exposure |
| url | http://www.journalssystem.com/aaem/Acute-hypersensitivity-pneumonitis-in-woodworkers-caused-by-the-inhalation-of-birch,114931,0,2.html |
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