Evaluation of corneal graft survival in mice model
<b>AIM:</b> To investigate the characteristics and criterion of graft rejection in mice model.<b>METHODS:</b> C57BL/6 or BALB/c mice corneal grafts were grafted onto BALB/c hosts. Each group was divided into two subgroups according to the corneal opacity scores 12d after tran...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2013-10-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2013/5/20130505.pdf |
| Summary: | <b>AIM:</b> To investigate the characteristics and criterion of graft rejection in mice model.<b>METHODS:</b> C57BL/6 or BALB/c mice corneal grafts were grafted onto BALB/c hosts. Each group was divided into two subgroups according to the corneal opacity scores 12d after transplantation. The characteristics of opacity and neovascularization were observed. Mice of the 12<sup>th</sup>, 50<sup>th</sup> day after transplantation, the grafts biopsy of mice in allogeneic group 1, which opacity score exceed 3, were prepared for histological observation and those restore transparent were endothelial stained.<b>RESULTS:</b> There was no difference of corneal opacity score on the 7<sup>th</sup> and 12<sup>th</sup> day after operation; the histological results had no disparity between syngeneic group and allogeneic group. On the 12<sup>th</sup> day after surgery, the turbidity curve was apparent in grafts with opacity score ＜2. Mononuclear cells were shown in grafts with opacity score reached 3 in allogeneic group 1. Different rejection performance was observed in tissue sections on the 50<sup>th</sup> day after surgery.<b>CONCLUSION:</b>Grafts, opacity score exceeds 3 from the 7<sup>th</sup> to the 12<sup>th</sup> day after operation could not be judged as a rejection. We should pay more attention to the variation of grafts opacity since 12d after corneal transplantation. |
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| ISSN: | 2222-3959 2227-4898 |