Evaluation of corneal graft survival in mice model
<b>AIM:</b> To investigate the characteristics and criterion of graft rejection in mice model.<b>METHODS:</b> C57BL/6 or BALB/c mice corneal grafts were grafted onto BALB/c hosts. Each group was divided into two subgroups according to the corneal opacity scores 12d after tran...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2013-10-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2013/5/20130505.pdf |
| _version_ | 1819237371746451456 |
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| author | Guo-Ling Chen Jing-Jing Zhang Jun Zhao Da-Jiang Wang Han Zhang |
| author_facet | Guo-Ling Chen Jing-Jing Zhang Jun Zhao Da-Jiang Wang Han Zhang |
| author_sort | Guo-Ling Chen |
| collection | DOAJ |
| description | <b>AIM:</b> To investigate the characteristics and criterion of graft rejection in mice model.<b>METHODS:</b> C57BL/6 or BALB/c mice corneal grafts were grafted onto BALB/c hosts. Each group was divided into two subgroups according to the corneal opacity scores 12d after transplantation. The characteristics of opacity and neovascularization were observed. Mice of the 12<sup>th</sup>, 50<sup>th</sup> day after transplantation, the grafts biopsy of mice in allogeneic group 1, which opacity score exceed 3, were prepared for histological observation and those restore transparent were endothelial stained.<b>RESULTS:</b> There was no difference of corneal opacity score on the 7<sup>th</sup> and 12<sup>th</sup> day after operation; the histological results had no disparity between syngeneic group and allogeneic group. On the 12<sup>th</sup> day after surgery, the turbidity curve was apparent in grafts with opacity score ＜2. Mononuclear cells were shown in grafts with opacity score reached 3 in allogeneic group 1. Different rejection performance was observed in tissue sections on the 50<sup>th</sup> day after surgery.<b>CONCLUSION:</b>Grafts, opacity score exceeds 3 from the 7<sup>th</sup> to the 12<sup>th</sup> day after operation could not be judged as a rejection. We should pay more attention to the variation of grafts opacity since 12d after corneal transplantation. |
| first_indexed | 2024-12-23T13:19:16Z |
| format | Article |
| id | doaj.art-e24331c3e01848d7bcf37564bfe04f4f |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-23T13:19:16Z |
| publishDate | 2013-10-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-e24331c3e01848d7bcf37564bfe04f4f2022-12-21T17:45:30ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982013-10-016557858310.3980/j.issn.2222-3959.2013.05.04Evaluation of corneal graft survival in mice modelGuo-Ling ChenJing-Jing ZhangJun ZhaoDa-Jiang WangHan Zhang<b>AIM:</b> To investigate the characteristics and criterion of graft rejection in mice model.<b>METHODS:</b> C57BL/6 or BALB/c mice corneal grafts were grafted onto BALB/c hosts. Each group was divided into two subgroups according to the corneal opacity scores 12d after transplantation. The characteristics of opacity and neovascularization were observed. Mice of the 12<sup>th</sup>, 50<sup>th</sup> day after transplantation, the grafts biopsy of mice in allogeneic group 1, which opacity score exceed 3, were prepared for histological observation and those restore transparent were endothelial stained.<b>RESULTS:</b> There was no difference of corneal opacity score on the 7<sup>th</sup> and 12<sup>th</sup> day after operation; the histological results had no disparity between syngeneic group and allogeneic group. On the 12<sup>th</sup> day after surgery, the turbidity curve was apparent in grafts with opacity score ＜2. Mononuclear cells were shown in grafts with opacity score reached 3 in allogeneic group 1. Different rejection performance was observed in tissue sections on the 50<sup>th</sup> day after surgery.<b>CONCLUSION:</b>Grafts, opacity score exceeds 3 from the 7<sup>th</sup> to the 12<sup>th</sup> day after operation could not be judged as a rejection. We should pay more attention to the variation of grafts opacity since 12d after corneal transplantation.http://www.ijo.cn/en_publish/2013/5/20130505.pdfcorneal transplantationgraft survivalexperimental study |
| spellingShingle | Guo-Ling Chen Jing-Jing Zhang Jun Zhao Da-Jiang Wang Han Zhang Evaluation of corneal graft survival in mice model International Journal of Ophthalmology corneal transplantation graft survival experimental study |
| title | Evaluation of corneal graft survival in mice model |
| title_full | Evaluation of corneal graft survival in mice model |
| title_fullStr | Evaluation of corneal graft survival in mice model |
| title_full_unstemmed | Evaluation of corneal graft survival in mice model |
| title_short | Evaluation of corneal graft survival in mice model |
| title_sort | evaluation of corneal graft survival in mice model |
| topic | corneal transplantation graft survival experimental study |
| url | http://www.ijo.cn/en_publish/2013/5/20130505.pdf |
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