Identification of the Group III <i>WRKY</i> Subfamily and the Functional Analysis of <i>GhWRKY53</i> in <i>Gossypium hirsutum</i> L.
WRKY transcription factors had multiple functions in plant secondary metabolism, leaf senescence, fruit ripening, adaptation to biotic and abiotic stress, and plant growth and development. However, knowledge of the group III <i>WRKY</i> subfamily in fiber development in upland cotton (&l...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/10/6/1235 |
| _version_ | 1797529800213004288 |
|---|---|
| author | Dongjie Yang Yuanyuan Liu Hailiang Cheng Qiaolian Wang Limin Lv Youping Zhang Guoli Song Dongyun Zuo |
| author_facet | Dongjie Yang Yuanyuan Liu Hailiang Cheng Qiaolian Wang Limin Lv Youping Zhang Guoli Song Dongyun Zuo |
| author_sort | Dongjie Yang |
| collection | DOAJ |
| description | WRKY transcription factors had multiple functions in plant secondary metabolism, leaf senescence, fruit ripening, adaptation to biotic and abiotic stress, and plant growth and development. However, knowledge of the group III <i>WRKY</i> subfamily in fiber development in upland cotton (<i>Gossypium hirsutum</i> L.) is largely absent. Previous studies have shown that there were 21 putative group III <i>WRKY</i> members in <i>G. hirsutum</i> L. These putative amino acid sequences from the III WRKY group were phylogenetically clustered into three clades. Multiple alignment, conservative motif analysis, and gene structure analysis showed that the members clustered together in the phylogenetic tree had similar motifs and gene structures. Expression pattern analysis revealed that variation in the expression levels of these genes in different tissues and fiber development stages. To better understand the functions of putative group III <i>WRKY</i> genes in <i>G. hirsutum</i> L., we selected the cotton fiber initiation-related gene <i>GhWRKY53</i> for cloning and functional identification. The subcellular localization experiment of <i>GhWRKY53</i> in <i>Nicotiana tabacum</i> leaves showed that it was located in the nucleus. The heterologous expression of <i>GhWRKY53</i> in <i>Arabidopsis thaliana</i> could significantly increase the density of trichomes. Twelve proteins that interacted with GhWRKY53 were screened from the cotton fiber cDNA library by yeast two-hybrid experiment. This study findings lay a foundation for further research on the role of the <i>GhWRKY53</i> during cotton fiber development and provide a new insight for further studying putative group III <i>WRKY</i> genes in <i>G. hirsutum</i> L. Our research results also provide vital information for the genetic mechanism of high-quality cotton fiber formation and essential genetic resources for cotton fiber quality improvement. |
| first_indexed | 2024-03-10T10:19:58Z |
| format | Article |
| id | doaj.art-e243726bce474aecab549e6986ec1ca5 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T10:19:58Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-e243726bce474aecab549e6986ec1ca52023-11-22T00:33:17ZengMDPI AGPlants2223-77472021-06-01106123510.3390/plants10061235Identification of the Group III <i>WRKY</i> Subfamily and the Functional Analysis of <i>GhWRKY53</i> in <i>Gossypium hirsutum</i> L.Dongjie Yang0Yuanyuan Liu1Hailiang Cheng2Qiaolian Wang3Limin Lv4Youping Zhang5Guoli Song6Dongyun Zuo7Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaWRKY transcription factors had multiple functions in plant secondary metabolism, leaf senescence, fruit ripening, adaptation to biotic and abiotic stress, and plant growth and development. However, knowledge of the group III <i>WRKY</i> subfamily in fiber development in upland cotton (<i>Gossypium hirsutum</i> L.) is largely absent. Previous studies have shown that there were 21 putative group III <i>WRKY</i> members in <i>G. hirsutum</i> L. These putative amino acid sequences from the III WRKY group were phylogenetically clustered into three clades. Multiple alignment, conservative motif analysis, and gene structure analysis showed that the members clustered together in the phylogenetic tree had similar motifs and gene structures. Expression pattern analysis revealed that variation in the expression levels of these genes in different tissues and fiber development stages. To better understand the functions of putative group III <i>WRKY</i> genes in <i>G. hirsutum</i> L., we selected the cotton fiber initiation-related gene <i>GhWRKY53</i> for cloning and functional identification. The subcellular localization experiment of <i>GhWRKY53</i> in <i>Nicotiana tabacum</i> leaves showed that it was located in the nucleus. The heterologous expression of <i>GhWRKY53</i> in <i>Arabidopsis thaliana</i> could significantly increase the density of trichomes. Twelve proteins that interacted with GhWRKY53 were screened from the cotton fiber cDNA library by yeast two-hybrid experiment. This study findings lay a foundation for further research on the role of the <i>GhWRKY53</i> during cotton fiber development and provide a new insight for further studying putative group III <i>WRKY</i> genes in <i>G. hirsutum</i> L. Our research results also provide vital information for the genetic mechanism of high-quality cotton fiber formation and essential genetic resources for cotton fiber quality improvement.https://www.mdpi.com/2223-7747/10/6/1235cottongene familyfiber initiationputative group III <i>WRKY</i> genesexpression pattern |
| spellingShingle | Dongjie Yang Yuanyuan Liu Hailiang Cheng Qiaolian Wang Limin Lv Youping Zhang Guoli Song Dongyun Zuo Identification of the Group III <i>WRKY</i> Subfamily and the Functional Analysis of <i>GhWRKY53</i> in <i>Gossypium hirsutum</i> L. Plants cotton gene family fiber initiation putative group III <i>WRKY</i> genes expression pattern |
| title | Identification of the Group III <i>WRKY</i> Subfamily and the Functional Analysis of <i>GhWRKY53</i> in <i>Gossypium hirsutum</i> L. |
| title_full | Identification of the Group III <i>WRKY</i> Subfamily and the Functional Analysis of <i>GhWRKY53</i> in <i>Gossypium hirsutum</i> L. |
| title_fullStr | Identification of the Group III <i>WRKY</i> Subfamily and the Functional Analysis of <i>GhWRKY53</i> in <i>Gossypium hirsutum</i> L. |
| title_full_unstemmed | Identification of the Group III <i>WRKY</i> Subfamily and the Functional Analysis of <i>GhWRKY53</i> in <i>Gossypium hirsutum</i> L. |
| title_short | Identification of the Group III <i>WRKY</i> Subfamily and the Functional Analysis of <i>GhWRKY53</i> in <i>Gossypium hirsutum</i> L. |
| title_sort | identification of the group iii i wrky i subfamily and the functional analysis of i ghwrky53 i in i gossypium hirsutum i l |
| topic | cotton gene family fiber initiation putative group III <i>WRKY</i> genes expression pattern |
| url | https://www.mdpi.com/2223-7747/10/6/1235 |
| work_keys_str_mv | AT dongjieyang identificationofthegroupiiiiwrkyisubfamilyandthefunctionalanalysisofighwrky53iinigossypiumhirsutumil AT yuanyuanliu identificationofthegroupiiiiwrkyisubfamilyandthefunctionalanalysisofighwrky53iinigossypiumhirsutumil AT hailiangcheng identificationofthegroupiiiiwrkyisubfamilyandthefunctionalanalysisofighwrky53iinigossypiumhirsutumil AT qiaolianwang identificationofthegroupiiiiwrkyisubfamilyandthefunctionalanalysisofighwrky53iinigossypiumhirsutumil AT liminlv identificationofthegroupiiiiwrkyisubfamilyandthefunctionalanalysisofighwrky53iinigossypiumhirsutumil AT youpingzhang identificationofthegroupiiiiwrkyisubfamilyandthefunctionalanalysisofighwrky53iinigossypiumhirsutumil AT guolisong identificationofthegroupiiiiwrkyisubfamilyandthefunctionalanalysisofighwrky53iinigossypiumhirsutumil AT dongyunzuo identificationofthegroupiiiiwrkyisubfamilyandthefunctionalanalysisofighwrky53iinigossypiumhirsutumil |