Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis
IgE antibody response in human strongyloidiasis was evaluated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting (IB) using Strongyloides ratti saline extract as heterologous antigen. A total of 50 serum samples of patients who were shedding S. stercoralis larvae in feces (group I, copr...
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Fundação Oswaldo Cruz (FIOCRUZ)
2004-02-01
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Series: | Memorias do Instituto Oswaldo Cruz |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000100016 |
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author | Rosângela Maria Rodrigues Mônica Camargo Sopelete Deise Aparecida de Oliveira Silva Jair Pereira Cunha-Júnior Ernesto Akio Taketomi Julia Maria Costa-Cruz |
author_facet | Rosângela Maria Rodrigues Mônica Camargo Sopelete Deise Aparecida de Oliveira Silva Jair Pereira Cunha-Júnior Ernesto Akio Taketomi Julia Maria Costa-Cruz |
author_sort | Rosângela Maria Rodrigues |
collection | DOAJ |
description | IgE antibody response in human strongyloidiasis was evaluated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting (IB) using Strongyloides ratti saline extract as heterologous antigen. A total of 50 serum samples of patients who were shedding S. stercoralis larvae in feces (group I, copropositive), 38 of patients with other intestinal parasites (group II), and 38 of subjects with negative results in three parasitologic assays (group III, copronegative) were analyzed. Levels of IgE anti-Strongyloides expressed in ELISA Index (EI) were significantly higher in patients of group I (1.32) than in group II (0.51) and group III (0.81), with positivity rates of 54%, 0%, and 10.5%, respectively. Fifteen S. ratti antigenic components were recognized in IB-IgE by sera of group I, with frequency ranging from 8% to 46%. In group II, only two antigenic bands (101, 81 kDa) were detected in a frequency of 10% and no reactivity was found in group III. Sera with EI values > 1.5 recognized five from 13 specific antigenic bands (70, 63, 61, 44, 7 kDa). It can be concluded that these five antigenic components recognized by IB-IgE using S. ratti antigen might be employed as an additional tool for improving the immunodiagnosis in human strongyloidiasis. |
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id | doaj.art-e24428442de94fa396393e0e85f358b7 |
institution | Directory Open Access Journal |
issn | 0074-0276 1678-8060 |
language | English |
last_indexed | 2024-03-12T08:13:23Z |
publishDate | 2004-02-01 |
publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
record_format | Article |
series | Memorias do Instituto Oswaldo Cruz |
spelling | doaj.art-e24428442de94fa396393e0e85f358b72023-09-02T19:06:31ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602004-02-01991899310.1590/S0074-02762004000100016Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasisRosângela Maria RodriguesMônica Camargo SopeleteDeise Aparecida de Oliveira SilvaJair Pereira Cunha-JúniorErnesto Akio TaketomiJulia Maria Costa-CruzIgE antibody response in human strongyloidiasis was evaluated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting (IB) using Strongyloides ratti saline extract as heterologous antigen. A total of 50 serum samples of patients who were shedding S. stercoralis larvae in feces (group I, copropositive), 38 of patients with other intestinal parasites (group II), and 38 of subjects with negative results in three parasitologic assays (group III, copronegative) were analyzed. Levels of IgE anti-Strongyloides expressed in ELISA Index (EI) were significantly higher in patients of group I (1.32) than in group II (0.51) and group III (0.81), with positivity rates of 54%, 0%, and 10.5%, respectively. Fifteen S. ratti antigenic components were recognized in IB-IgE by sera of group I, with frequency ranging from 8% to 46%. In group II, only two antigenic bands (101, 81 kDa) were detected in a frequency of 10% and no reactivity was found in group III. Sera with EI values > 1.5 recognized five from 13 specific antigenic bands (70, 63, 61, 44, 7 kDa). It can be concluded that these five antigenic components recognized by IB-IgE using S. ratti antigen might be employed as an additional tool for improving the immunodiagnosis in human strongyloidiasis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000100016strongyloidiasisStrongyloides rattiIgEimmunoblottingELISA |
spellingShingle | Rosângela Maria Rodrigues Mônica Camargo Sopelete Deise Aparecida de Oliveira Silva Jair Pereira Cunha-Júnior Ernesto Akio Taketomi Julia Maria Costa-Cruz Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis Memorias do Instituto Oswaldo Cruz strongyloidiasis Strongyloides ratti IgE immunoblotting ELISA |
title | Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis |
title_full | Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis |
title_fullStr | Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis |
title_full_unstemmed | Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis |
title_short | Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis |
title_sort | strongyloides ratti antigenic components recognized by ige antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis |
topic | strongyloidiasis Strongyloides ratti IgE immunoblotting ELISA |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000100016 |
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