Antigenotoxic, antimutagenic and cytoprotective potential of Salvia hispanica L. seed extract on human leukocytes exposed to oxidative damage
This study assesses the antigenotoxic activity, antimutagenic and inhibiting cytotoxic conferred for the extract of seeds of Salvia hispanica L. (ShE) in culture of human leukocytes. Phytochemical methods were used by HPLC for determination of compounds: chlorogenic acid, caffeic acid, gallic acid,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-11-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617305728 |
| _version_ | 1819020834934620160 |
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| author | Emanoeli da Rosa Queila Daiane Fonseca do Amaral Jonathaline Apollo Duarte Jéssica Tamara Limberger Pamella Eduardha Espindola Chaves Luísa Zuravski Luís Flávio Souza de Oliveira Michel Mansur Machado |
| author_facet | Emanoeli da Rosa Queila Daiane Fonseca do Amaral Jonathaline Apollo Duarte Jéssica Tamara Limberger Pamella Eduardha Espindola Chaves Luísa Zuravski Luís Flávio Souza de Oliveira Michel Mansur Machado |
| author_sort | Emanoeli da Rosa |
| collection | DOAJ |
| description | This study assesses the antigenotoxic activity, antimutagenic and inhibiting cytotoxic conferred for the extract of seeds of Salvia hispanica L. (ShE) in culture of human leukocytes. Phytochemical methods were used by HPLC for determination of compounds: chlorogenic acid, caffeic acid, gallic acid, kaempferol, quercetin and rutin. Cellular cultures were placed in contact with the extract at concentrations of 100 µg/mL and 10 µg/mL in addition to hydrogen peroxide as causative agent of damage. The results showed a reversal rate of DNA damage of 31%, 41.4% in the frequency of micronuclei, 21.7% in cytoplasmic rates of chromosomal abnormalities and a 17.7% improvement in cell viability in the sample containing 100 µg/mL of ShE. The samples that contained the ShE in low concentration of 10 µg/mL did not show such reversals against the effects of H2O2. We demonstrated that the use of ShE has beneficial and promising effects. |
| first_indexed | 2024-12-21T03:57:31Z |
| format | Article |
| id | doaj.art-e249ca18afe94d69bc5eb4ecdd883a2a |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-21T03:57:31Z |
| publishDate | 2017-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e249ca18afe94d69bc5eb4ecdd883a2a2022-12-21T19:16:48ZengElsevierJournal of Functional Foods1756-46462017-11-0138505509Antigenotoxic, antimutagenic and cytoprotective potential of Salvia hispanica L. seed extract on human leukocytes exposed to oxidative damageEmanoeli da Rosa0Queila Daiane Fonseca do Amaral1Jonathaline Apollo Duarte2Jéssica Tamara Limberger3Pamella Eduardha Espindola Chaves4Luísa Zuravski5Luís Flávio Souza de Oliveira6Michel Mansur Machado7TOXCEL – Grupo de Pesquisa em Toxicologia Celular, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas, Curso de Farmácia, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, BrazilTOXCEL – Grupo de Pesquisa em Toxicologia Celular, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas, Curso de Farmácia, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Curso de Farmácia, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, BrazilTOXCEL – Grupo de Pesquisa em Toxicologia Celular, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, BrazilTOXCEL – Grupo de Pesquisa em Toxicologia Celular, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, BrazilTOXCEL – Grupo de Pesquisa em Toxicologia Celular, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, BrazilTOXCEL – Grupo de Pesquisa em Toxicologia Celular, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas, Curso de Farmácia, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, BrazilTOXCEL – Grupo de Pesquisa em Toxicologia Celular, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas, Curso de Farmácia, Universidade Federal do Pampa, BR 472, Km 592, Caixa Postal 118, CEP 97.500-970 Uruguaiana, RS, Brazil; Corresponding author.This study assesses the antigenotoxic activity, antimutagenic and inhibiting cytotoxic conferred for the extract of seeds of Salvia hispanica L. (ShE) in culture of human leukocytes. Phytochemical methods were used by HPLC for determination of compounds: chlorogenic acid, caffeic acid, gallic acid, kaempferol, quercetin and rutin. Cellular cultures were placed in contact with the extract at concentrations of 100 µg/mL and 10 µg/mL in addition to hydrogen peroxide as causative agent of damage. The results showed a reversal rate of DNA damage of 31%, 41.4% in the frequency of micronuclei, 21.7% in cytoplasmic rates of chromosomal abnormalities and a 17.7% improvement in cell viability in the sample containing 100 µg/mL of ShE. The samples that contained the ShE in low concentration of 10 µg/mL did not show such reversals against the effects of H2O2. We demonstrated that the use of ShE has beneficial and promising effects.http://www.sciencedirect.com/science/article/pii/S1756464617305728GenotoxicMutagenicChiaSalvia hispanicaCell culture |
| spellingShingle | Emanoeli da Rosa Queila Daiane Fonseca do Amaral Jonathaline Apollo Duarte Jéssica Tamara Limberger Pamella Eduardha Espindola Chaves Luísa Zuravski Luís Flávio Souza de Oliveira Michel Mansur Machado Antigenotoxic, antimutagenic and cytoprotective potential of Salvia hispanica L. seed extract on human leukocytes exposed to oxidative damage Journal of Functional Foods Genotoxic Mutagenic Chia Salvia hispanica Cell culture |
| title | Antigenotoxic, antimutagenic and cytoprotective potential of Salvia hispanica L. seed extract on human leukocytes exposed to oxidative damage |
| title_full | Antigenotoxic, antimutagenic and cytoprotective potential of Salvia hispanica L. seed extract on human leukocytes exposed to oxidative damage |
| title_fullStr | Antigenotoxic, antimutagenic and cytoprotective potential of Salvia hispanica L. seed extract on human leukocytes exposed to oxidative damage |
| title_full_unstemmed | Antigenotoxic, antimutagenic and cytoprotective potential of Salvia hispanica L. seed extract on human leukocytes exposed to oxidative damage |
| title_short | Antigenotoxic, antimutagenic and cytoprotective potential of Salvia hispanica L. seed extract on human leukocytes exposed to oxidative damage |
| title_sort | antigenotoxic antimutagenic and cytoprotective potential of salvia hispanica l seed extract on human leukocytes exposed to oxidative damage |
| topic | Genotoxic Mutagenic Chia Salvia hispanica Cell culture |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617305728 |
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