Downregulation of miR‐29b‐3p aggravates podocyte injury by targeting HDAC4 in LPS‐induced acute kidney injury
Abstract Sepsis is a severe organ dysfunction disease, usually accompanied by acute kidney injury (AKI). miR‐29b‐3p was inhibited in sepsis‐induced AKI, while its role in AKI was unclear. Therefore, this study determined the role of miR‐29b‐3p in sepsis‐induced AKI, and investigated its underlying m...
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| Format: | Article |
| Language: | English |
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Wiley
2021-12-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12431 |
| _version_ | 1818735517076815872 |
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| author | Zong‐Lan Ha Zi‐Ying Yu |
| author_facet | Zong‐Lan Ha Zi‐Ying Yu |
| author_sort | Zong‐Lan Ha |
| collection | DOAJ |
| description | Abstract Sepsis is a severe organ dysfunction disease, usually accompanied by acute kidney injury (AKI). miR‐29b‐3p was inhibited in sepsis‐induced AKI, while its role in AKI was unclear. Therefore, this study determined the role of miR‐29b‐3p in sepsis‐induced AKI, and investigated its underlying mechanism. In this study, the AKI model was established through injecting with lipopolysaccharides (LPS) intraperitoneally. In LPS challenged mice, serum blood urea nitrogen and creatinine were increased, and renal tissues pathological damage was induced. Besides, miR‐29b‐3p was declined in LPS‐induced AKI mice and podocytes. In addition, HDAC4 was elevated in LPS‐treated podocytes. Furthermore, upregulated miR‐29b‐3p attenuated LPS‐induced mice podocyte injury, and HDAC4 was identified as a direct target of miR‐29b‐3p. Moreover, overexpression of miR‐29b‐3p attenuated LPS‐induced AKI in mice. In conclusion, miR‐29b‐3p was inhibited in LPS‐induced AKI. Downregulation of miR‐29b‐3p aggravated podocyte injury through targeting HDAC4 in LPS‐induced AKI. miR‐29b‐3p may act as a valuable target for AKI therapy. |
| first_indexed | 2024-12-18T00:22:30Z |
| format | Article |
| id | doaj.art-e24f063d2a984adc94eb6f36289c487a |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-12-18T00:22:30Z |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-e24f063d2a984adc94eb6f36289c487a2022-12-21T21:27:18ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502021-12-0137121069107610.1002/kjm2.12431Downregulation of miR‐29b‐3p aggravates podocyte injury by targeting HDAC4 in LPS‐induced acute kidney injuryZong‐Lan Ha0Zi‐Ying Yu1Department of Critical Care Medicine Qinghai University Affiliated Hospital Xining ChinaDepartment of Emergency The First Affiliated Hospital of University of South China Hengyang ChinaAbstract Sepsis is a severe organ dysfunction disease, usually accompanied by acute kidney injury (AKI). miR‐29b‐3p was inhibited in sepsis‐induced AKI, while its role in AKI was unclear. Therefore, this study determined the role of miR‐29b‐3p in sepsis‐induced AKI, and investigated its underlying mechanism. In this study, the AKI model was established through injecting with lipopolysaccharides (LPS) intraperitoneally. In LPS challenged mice, serum blood urea nitrogen and creatinine were increased, and renal tissues pathological damage was induced. Besides, miR‐29b‐3p was declined in LPS‐induced AKI mice and podocytes. In addition, HDAC4 was elevated in LPS‐treated podocytes. Furthermore, upregulated miR‐29b‐3p attenuated LPS‐induced mice podocyte injury, and HDAC4 was identified as a direct target of miR‐29b‐3p. Moreover, overexpression of miR‐29b‐3p attenuated LPS‐induced AKI in mice. In conclusion, miR‐29b‐3p was inhibited in LPS‐induced AKI. Downregulation of miR‐29b‐3p aggravated podocyte injury through targeting HDAC4 in LPS‐induced AKI. miR‐29b‐3p may act as a valuable target for AKI therapy.https://doi.org/10.1002/kjm2.12431acute kidney injuryHDAC4LPSmiR‐29b‐3psepsis |
| spellingShingle | Zong‐Lan Ha Zi‐Ying Yu Downregulation of miR‐29b‐3p aggravates podocyte injury by targeting HDAC4 in LPS‐induced acute kidney injury Kaohsiung Journal of Medical Sciences acute kidney injury HDAC4 LPS miR‐29b‐3p sepsis |
| title | Downregulation of miR‐29b‐3p aggravates podocyte injury by targeting HDAC4 in LPS‐induced acute kidney injury |
| title_full | Downregulation of miR‐29b‐3p aggravates podocyte injury by targeting HDAC4 in LPS‐induced acute kidney injury |
| title_fullStr | Downregulation of miR‐29b‐3p aggravates podocyte injury by targeting HDAC4 in LPS‐induced acute kidney injury |
| title_full_unstemmed | Downregulation of miR‐29b‐3p aggravates podocyte injury by targeting HDAC4 in LPS‐induced acute kidney injury |
| title_short | Downregulation of miR‐29b‐3p aggravates podocyte injury by targeting HDAC4 in LPS‐induced acute kidney injury |
| title_sort | downregulation of mir 29b 3p aggravates podocyte injury by targeting hdac4 in lps induced acute kidney injury |
| topic | acute kidney injury HDAC4 LPS miR‐29b‐3p sepsis |
| url | https://doi.org/10.1002/kjm2.12431 |
| work_keys_str_mv | AT zonglanha downregulationofmir29b3paggravatespodocyteinjurybytargetinghdac4inlpsinducedacutekidneyinjury AT ziyingyu downregulationofmir29b3paggravatespodocyteinjurybytargetinghdac4inlpsinducedacutekidneyinjury |