Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells

<p>Abstract</p> <p>Background</p> <p>The interaction of nuclear and mitochondrial genes is an essential feature in maintenance of normal cellular function. Of 82 structural subunits that make up the oxidative phosphorylation system in the mitochondria, mitochondrial DNA...

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Main Authors: Gabrielson Edward, Anbazhagan Ramaswamy, Kachhap Sushant, Delsite Robert, Singh Keshav K
Format: Article
Language:English
Published: BMC 2002-11-01
Series:Molecular Cancer
Subjects:
Online Access:http://www.molecular-cancer.com/content/1/1/6
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author Gabrielson Edward
Anbazhagan Ramaswamy
Kachhap Sushant
Delsite Robert
Singh Keshav K
author_facet Gabrielson Edward
Anbazhagan Ramaswamy
Kachhap Sushant
Delsite Robert
Singh Keshav K
author_sort Gabrielson Edward
collection DOAJ
description <p>Abstract</p> <p>Background</p> <p>The interaction of nuclear and mitochondrial genes is an essential feature in maintenance of normal cellular function. Of 82 structural subunits that make up the oxidative phosphorylation system in the mitochondria, mitochondrial DNA (mtDNA) encodes 13 subunits and rest of the subunits are encoded by nuclear DNA. Mutations in mitochondrial genes encoding the 13 subunits have been reported in a variety of cancers. However, little is known about the nuclear response to impairment of mitochondrial function in human cells.</p> <p>Results</p> <p>We isolated a Rho<sup>0</sup> (devoid of mtDNA) derivative of a breast cancer cell line. Our study suggests that depletion of mtDNA results in oxidative stress, causing increased lipid peroxidation in breast cancer cells. Using a cDNA microarray we compared differences in the nuclear gene expression profile between a breast cancer cell line (parental Rho<sup>+</sup>) and its Rho<sup>0</sup> derivative impaired in mitochondrial function. Expression of several nuclear genes involved in cell signaling, cell architecture, energy metabolism, cell growth, apoptosis including general transcription factor TFIIH, v-maf, AML1, was induced in Rho<sup>0</sup> cells. Expression of several genes was also down regulated. These include phospholipase C, agouti related protein, PKC gamma, protein tyrosine phosphatase C, phosphodiestarase 1A (cell signaling), PIBF1, cytochrome p450, (metabolism) and cyclin dependent kinase inhibitor p19, and GAP43 (cell growth and differentiation).</p> <p>Conclusions</p> <p>Mitochondrial impairment in breast cancer cells results in altered expression of nuclear genes involved in signaling, cellular architecture, metabolism, cell growth and differentiation, and apoptosis. These genes may mediate the cross talk between mitochondria and the nucleus.</p>
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spelling doaj.art-e2505aecc30b4d8cad303d6d2492b61e2022-12-22T00:27:20ZengBMCMolecular Cancer1476-45982002-11-0111610.1186/1476-4598-1-6Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cellsGabrielson EdwardAnbazhagan RamaswamyKachhap SushantDelsite RobertSingh Keshav K<p>Abstract</p> <p>Background</p> <p>The interaction of nuclear and mitochondrial genes is an essential feature in maintenance of normal cellular function. Of 82 structural subunits that make up the oxidative phosphorylation system in the mitochondria, mitochondrial DNA (mtDNA) encodes 13 subunits and rest of the subunits are encoded by nuclear DNA. Mutations in mitochondrial genes encoding the 13 subunits have been reported in a variety of cancers. However, little is known about the nuclear response to impairment of mitochondrial function in human cells.</p> <p>Results</p> <p>We isolated a Rho<sup>0</sup> (devoid of mtDNA) derivative of a breast cancer cell line. Our study suggests that depletion of mtDNA results in oxidative stress, causing increased lipid peroxidation in breast cancer cells. Using a cDNA microarray we compared differences in the nuclear gene expression profile between a breast cancer cell line (parental Rho<sup>+</sup>) and its Rho<sup>0</sup> derivative impaired in mitochondrial function. Expression of several nuclear genes involved in cell signaling, cell architecture, energy metabolism, cell growth, apoptosis including general transcription factor TFIIH, v-maf, AML1, was induced in Rho<sup>0</sup> cells. Expression of several genes was also down regulated. These include phospholipase C, agouti related protein, PKC gamma, protein tyrosine phosphatase C, phosphodiestarase 1A (cell signaling), PIBF1, cytochrome p450, (metabolism) and cyclin dependent kinase inhibitor p19, and GAP43 (cell growth and differentiation).</p> <p>Conclusions</p> <p>Mitochondrial impairment in breast cancer cells results in altered expression of nuclear genes involved in signaling, cellular architecture, metabolism, cell growth and differentiation, and apoptosis. These genes may mediate the cross talk between mitochondria and the nucleus.</p>http://www.molecular-cancer.com/content/1/1/6MitochondriaMitochondrial DNANuclear genesBreast CancerCancer
spellingShingle Gabrielson Edward
Anbazhagan Ramaswamy
Kachhap Sushant
Delsite Robert
Singh Keshav K
Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells
Molecular Cancer
Mitochondria
Mitochondrial DNA
Nuclear genes
Breast Cancer
Cancer
title Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells
title_full Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells
title_fullStr Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells
title_full_unstemmed Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells
title_short Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells
title_sort nuclear genes involved in mitochondria to nucleus communication in breast cancer cells
topic Mitochondria
Mitochondrial DNA
Nuclear genes
Breast Cancer
Cancer
url http://www.molecular-cancer.com/content/1/1/6
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AT anbazhaganramaswamy nucleargenesinvolvedinmitochondriatonucleuscommunicationinbreastcancercells
AT kachhapsushant nucleargenesinvolvedinmitochondriatonucleuscommunicationinbreastcancercells
AT delsiterobert nucleargenesinvolvedinmitochondriatonucleuscommunicationinbreastcancercells
AT singhkeshavk nucleargenesinvolvedinmitochondriatonucleuscommunicationinbreastcancercells