Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells
<p>Abstract</p> <p>Background</p> <p>The interaction of nuclear and mitochondrial genes is an essential feature in maintenance of normal cellular function. Of 82 structural subunits that make up the oxidative phosphorylation system in the mitochondria, mitochondrial DNA...
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Format: | Article |
Language: | English |
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BMC
2002-11-01
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Series: | Molecular Cancer |
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Online Access: | http://www.molecular-cancer.com/content/1/1/6 |
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author | Gabrielson Edward Anbazhagan Ramaswamy Kachhap Sushant Delsite Robert Singh Keshav K |
author_facet | Gabrielson Edward Anbazhagan Ramaswamy Kachhap Sushant Delsite Robert Singh Keshav K |
author_sort | Gabrielson Edward |
collection | DOAJ |
description | <p>Abstract</p> <p>Background</p> <p>The interaction of nuclear and mitochondrial genes is an essential feature in maintenance of normal cellular function. Of 82 structural subunits that make up the oxidative phosphorylation system in the mitochondria, mitochondrial DNA (mtDNA) encodes 13 subunits and rest of the subunits are encoded by nuclear DNA. Mutations in mitochondrial genes encoding the 13 subunits have been reported in a variety of cancers. However, little is known about the nuclear response to impairment of mitochondrial function in human cells.</p> <p>Results</p> <p>We isolated a Rho<sup>0</sup> (devoid of mtDNA) derivative of a breast cancer cell line. Our study suggests that depletion of mtDNA results in oxidative stress, causing increased lipid peroxidation in breast cancer cells. Using a cDNA microarray we compared differences in the nuclear gene expression profile between a breast cancer cell line (parental Rho<sup>+</sup>) and its Rho<sup>0</sup> derivative impaired in mitochondrial function. Expression of several nuclear genes involved in cell signaling, cell architecture, energy metabolism, cell growth, apoptosis including general transcription factor TFIIH, v-maf, AML1, was induced in Rho<sup>0</sup> cells. Expression of several genes was also down regulated. These include phospholipase C, agouti related protein, PKC gamma, protein tyrosine phosphatase C, phosphodiestarase 1A (cell signaling), PIBF1, cytochrome p450, (metabolism) and cyclin dependent kinase inhibitor p19, and GAP43 (cell growth and differentiation).</p> <p>Conclusions</p> <p>Mitochondrial impairment in breast cancer cells results in altered expression of nuclear genes involved in signaling, cellular architecture, metabolism, cell growth and differentiation, and apoptosis. These genes may mediate the cross talk between mitochondria and the nucleus.</p> |
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format | Article |
id | doaj.art-e2505aecc30b4d8cad303d6d2492b61e |
institution | Directory Open Access Journal |
issn | 1476-4598 |
language | English |
last_indexed | 2024-12-12T10:31:26Z |
publishDate | 2002-11-01 |
publisher | BMC |
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series | Molecular Cancer |
spelling | doaj.art-e2505aecc30b4d8cad303d6d2492b61e2022-12-22T00:27:20ZengBMCMolecular Cancer1476-45982002-11-0111610.1186/1476-4598-1-6Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cellsGabrielson EdwardAnbazhagan RamaswamyKachhap SushantDelsite RobertSingh Keshav K<p>Abstract</p> <p>Background</p> <p>The interaction of nuclear and mitochondrial genes is an essential feature in maintenance of normal cellular function. Of 82 structural subunits that make up the oxidative phosphorylation system in the mitochondria, mitochondrial DNA (mtDNA) encodes 13 subunits and rest of the subunits are encoded by nuclear DNA. Mutations in mitochondrial genes encoding the 13 subunits have been reported in a variety of cancers. However, little is known about the nuclear response to impairment of mitochondrial function in human cells.</p> <p>Results</p> <p>We isolated a Rho<sup>0</sup> (devoid of mtDNA) derivative of a breast cancer cell line. Our study suggests that depletion of mtDNA results in oxidative stress, causing increased lipid peroxidation in breast cancer cells. Using a cDNA microarray we compared differences in the nuclear gene expression profile between a breast cancer cell line (parental Rho<sup>+</sup>) and its Rho<sup>0</sup> derivative impaired in mitochondrial function. Expression of several nuclear genes involved in cell signaling, cell architecture, energy metabolism, cell growth, apoptosis including general transcription factor TFIIH, v-maf, AML1, was induced in Rho<sup>0</sup> cells. Expression of several genes was also down regulated. These include phospholipase C, agouti related protein, PKC gamma, protein tyrosine phosphatase C, phosphodiestarase 1A (cell signaling), PIBF1, cytochrome p450, (metabolism) and cyclin dependent kinase inhibitor p19, and GAP43 (cell growth and differentiation).</p> <p>Conclusions</p> <p>Mitochondrial impairment in breast cancer cells results in altered expression of nuclear genes involved in signaling, cellular architecture, metabolism, cell growth and differentiation, and apoptosis. These genes may mediate the cross talk between mitochondria and the nucleus.</p>http://www.molecular-cancer.com/content/1/1/6MitochondriaMitochondrial DNANuclear genesBreast CancerCancer |
spellingShingle | Gabrielson Edward Anbazhagan Ramaswamy Kachhap Sushant Delsite Robert Singh Keshav K Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells Molecular Cancer Mitochondria Mitochondrial DNA Nuclear genes Breast Cancer Cancer |
title | Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells |
title_full | Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells |
title_fullStr | Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells |
title_full_unstemmed | Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells |
title_short | Nuclear genes involved in mitochondria-to-nucleus communication in breast cancer cells |
title_sort | nuclear genes involved in mitochondria to nucleus communication in breast cancer cells |
topic | Mitochondria Mitochondrial DNA Nuclear genes Breast Cancer Cancer |
url | http://www.molecular-cancer.com/content/1/1/6 |
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