Can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds?
Abstract: Sunflower (Helianthus annuus L.) is a relevant oilseed species used as feed in human and animal nutrition and in multiple industrial applications. However, oilseeds need to deal with the loss of vigor when used as a propagule, due to loss of seed physiological quality (viability and vigor)...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Tecnologia de Sementes
2022-04-01
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| Series: | Journal of Seed Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372022000100111&tlng=en |
| _version_ | 1818274930687475712 |
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| author | Aline Aparecida Silva Pereira Fernanda Carlota Nery Rafael Agostinho Ferreira Victor Navarro da Silva Mateus Moreira Bernardes Heloisa Oliveira dos Santos Elisa Monteze Bicalho |
| author_facet | Aline Aparecida Silva Pereira Fernanda Carlota Nery Rafael Agostinho Ferreira Victor Navarro da Silva Mateus Moreira Bernardes Heloisa Oliveira dos Santos Elisa Monteze Bicalho |
| author_sort | Aline Aparecida Silva Pereira |
| collection | DOAJ |
| description | Abstract: Sunflower (Helianthus annuus L.) is a relevant oilseed species used as feed in human and animal nutrition and in multiple industrial applications. However, oilseeds need to deal with the loss of vigor when used as a propagule, due to loss of seed physiological quality (viability and vigor) caused by deterioration events. We have evaluated in the present study the effects of seeds priming techniques (water, ascorbic acid, and sodium nitroprusside) on vigor, germination, antioxidant enzymes and membrane integrity of stored sunflower hybrid HELIO 251 seeds. Germination parameters showed that non-primed seeds (control) delayed germination in almost 72 h while all primed seeds germinated 100% in 24 h. Electrolyte leakage and malondialdehyde levels were higher in non-primed seeds indicating higher degree of membrane damage. An increase in the activity of catalase and ascorbate peroxidase was also observed after 8 h of imbibition of primed seeds compared to non-primed seeds. Regarding superoxide dismutase, there was no significant differences between treatments after 8 h of imbibition, whereas the highest activity was after 24 h of imbibition in non-primed seeds. Therefore, it can be concluded priming with ascorbic acid or sodium nitroprusside exhibited better performance in germination of stored sunflower seeds, probably resulting from lower accumulation of reactive oxygen species and consequent reduced oxidative damage due to an efficient antioxidant enzyme system. |
| first_indexed | 2024-12-12T22:21:41Z |
| format | Article |
| id | doaj.art-e2519e18bb1b410f8bd9c67ad4de848c |
| institution | Directory Open Access Journal |
| issn | 2317-1545 |
| language | English |
| last_indexed | 2024-12-12T22:21:41Z |
| publishDate | 2022-04-01 |
| publisher | Associação Brasileira de Tecnologia de Sementes |
| record_format | Article |
| series | Journal of Seed Science |
| spelling | doaj.art-e2519e18bb1b410f8bd9c67ad4de848c2022-12-22T00:09:54ZengAssociação Brasileira de Tecnologia de SementesJournal of Seed Science2317-15452022-04-014410.1590/2317-1545v44256600Can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds?Aline Aparecida Silva Pereirahttps://orcid.org/0000-0001-8052-8603Fernanda Carlota Neryhttps://orcid.org/0000-0003-0021-3973Rafael Agostinho Ferreirahttps://orcid.org/0000-0002-1591-9082Victor Navarro da Silvahttps://orcid.org/0000-0003-3636-2970Mateus Moreira Bernardeshttps://orcid.org/0000-0002-3487-0966Heloisa Oliveira dos Santoshttps://orcid.org/0000-0003-1384-4969Elisa Monteze Bicalhohttps://orcid.org/0000-0003-1746-0663Abstract: Sunflower (Helianthus annuus L.) is a relevant oilseed species used as feed in human and animal nutrition and in multiple industrial applications. However, oilseeds need to deal with the loss of vigor when used as a propagule, due to loss of seed physiological quality (viability and vigor) caused by deterioration events. We have evaluated in the present study the effects of seeds priming techniques (water, ascorbic acid, and sodium nitroprusside) on vigor, germination, antioxidant enzymes and membrane integrity of stored sunflower hybrid HELIO 251 seeds. Germination parameters showed that non-primed seeds (control) delayed germination in almost 72 h while all primed seeds germinated 100% in 24 h. Electrolyte leakage and malondialdehyde levels were higher in non-primed seeds indicating higher degree of membrane damage. An increase in the activity of catalase and ascorbate peroxidase was also observed after 8 h of imbibition of primed seeds compared to non-primed seeds. Regarding superoxide dismutase, there was no significant differences between treatments after 8 h of imbibition, whereas the highest activity was after 24 h of imbibition in non-primed seeds. Therefore, it can be concluded priming with ascorbic acid or sodium nitroprusside exhibited better performance in germination of stored sunflower seeds, probably resulting from lower accumulation of reactive oxygen species and consequent reduced oxidative damage due to an efficient antioxidant enzyme system.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372022000100111&tlng=enagingoilseedoxidative damageprimingseed vigor |
| spellingShingle | Aline Aparecida Silva Pereira Fernanda Carlota Nery Rafael Agostinho Ferreira Victor Navarro da Silva Mateus Moreira Bernardes Heloisa Oliveira dos Santos Elisa Monteze Bicalho Can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds? Journal of Seed Science aging oilseed oxidative damage priming seed vigor |
| title | Can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds? |
| title_full | Can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds? |
| title_fullStr | Can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds? |
| title_full_unstemmed | Can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds? |
| title_short | Can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds? |
| title_sort | can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds |
| topic | aging oilseed oxidative damage priming seed vigor |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372022000100111&tlng=en |
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