Analyses of fin ray types to detect strontium markers in juvenile blunt-snout bream Megalobrama amblycephala
Abstract Background Restocking by introducing hatchery-reared fish into wild habitats aids in the restoration of fishery aquatic ecosystems and reefs to increase the abundance of fish resources, restore the ecological balance of water bodies, and enhance ecosystem functioning. Accurately, rapidly, a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024-02-01
|
| Series: | Ecological Processes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13717-024-00501-z |
| _version_ | 1797275884850249728 |
|---|---|
| author | Yahua Zhu Tao Jiang Xiubao Chen Hongbo Liu Quinton Phelps Jian Yang |
| author_facet | Yahua Zhu Tao Jiang Xiubao Chen Hongbo Liu Quinton Phelps Jian Yang |
| author_sort | Yahua Zhu |
| collection | DOAJ |
| description | Abstract Background Restocking by introducing hatchery-reared fish into wild habitats aids in the restoration of fishery aquatic ecosystems and reefs to increase the abundance of fish resources, restore the ecological balance of water bodies, and enhance ecosystem functioning. Accurately, rapidly, and effectively evaluating the success of restocking using chemical markers (e.g., strontium [Sr]) remains challenging for fisheries management. Consequently, for non-lethal fish sampling, hard tissues, such as fin rays, have received increasing attention as a target for marking method. However, data on the differences in marking different types of fin rays remain limited. Therefore, we exposed juvenile blunt snout bream individuals (Megalobrama amblycephala) to 0 (control group) or 800 mg/L of SrCl2·6H2O (marked group) for 5 days and transferred them into normal aerated water for post-immersion culture. We sampled their pectoral, dorsal, ventral, anal, and caudal fin rays. The Sr marks among the fin types were sampled at 0 and 20 days post-immersion and evaluated using an electron probe micro-analyzer (EPMA) for the five-day Sr/Ca ratios, along with line transect and Sr mapping analyses. Results Sr marking signatures were observed in all fin types in the marked group, with a success rate of up to 100%. Although marking efficiency varied among the different fin ray types, the highest Sr/Ca ratios were most often detected in the dorsal fin. Cross-sectional Sr concentration maps of all fin rays sampled showed high-Sr domains in the marked group; in contrast, the entire cross-sections of the control group displayed low Sr contents, indicating successful marking efficiency. Conclusions Fin ray Sr marking is a successful method for juvenile M. amblycephala, with the advantages of non-lethality and negligible sampling injuries, facilitating the rapid and effective evaluation of Sr marking in restocking M. amblycephala. |
| first_indexed | 2024-03-07T15:20:25Z |
| format | Article |
| id | doaj.art-e25453f66c08432f9d0b543ce2d914e0 |
| institution | Directory Open Access Journal |
| issn | 2192-1709 |
| language | English |
| last_indexed | 2024-03-07T15:20:25Z |
| publishDate | 2024-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Ecological Processes |
| spelling | doaj.art-e25453f66c08432f9d0b543ce2d914e02024-03-05T17:42:13ZengSpringerOpenEcological Processes2192-17092024-02-0113111110.1186/s13717-024-00501-zAnalyses of fin ray types to detect strontium markers in juvenile blunt-snout bream Megalobrama amblycephalaYahua Zhu0Tao Jiang1Xiubao Chen2Hongbo Liu3Quinton Phelps4Jian Yang5Wuxi Fisheries College, Nanjing Agricultural University (WFCNAU)Laboratory of Fishery Microchemistry, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences (FFRCCAFS)Laboratory of Fishery Microchemistry, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences (FFRCCAFS)Laboratory of Fishery Microchemistry, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences (FFRCCAFS)Department of Biology, Missouri State University (DBMSU)Wuxi Fisheries College, Nanjing Agricultural University (WFCNAU)Abstract Background Restocking by introducing hatchery-reared fish into wild habitats aids in the restoration of fishery aquatic ecosystems and reefs to increase the abundance of fish resources, restore the ecological balance of water bodies, and enhance ecosystem functioning. Accurately, rapidly, and effectively evaluating the success of restocking using chemical markers (e.g., strontium [Sr]) remains challenging for fisheries management. Consequently, for non-lethal fish sampling, hard tissues, such as fin rays, have received increasing attention as a target for marking method. However, data on the differences in marking different types of fin rays remain limited. Therefore, we exposed juvenile blunt snout bream individuals (Megalobrama amblycephala) to 0 (control group) or 800 mg/L of SrCl2·6H2O (marked group) for 5 days and transferred them into normal aerated water for post-immersion culture. We sampled their pectoral, dorsal, ventral, anal, and caudal fin rays. The Sr marks among the fin types were sampled at 0 and 20 days post-immersion and evaluated using an electron probe micro-analyzer (EPMA) for the five-day Sr/Ca ratios, along with line transect and Sr mapping analyses. Results Sr marking signatures were observed in all fin types in the marked group, with a success rate of up to 100%. Although marking efficiency varied among the different fin ray types, the highest Sr/Ca ratios were most often detected in the dorsal fin. Cross-sectional Sr concentration maps of all fin rays sampled showed high-Sr domains in the marked group; in contrast, the entire cross-sections of the control group displayed low Sr contents, indicating successful marking efficiency. Conclusions Fin ray Sr marking is a successful method for juvenile M. amblycephala, with the advantages of non-lethality and negligible sampling injuries, facilitating the rapid and effective evaluation of Sr marking in restocking M. amblycephala.https://doi.org/10.1186/s13717-024-00501-zMegalobrama amblycephalaJuvenileFin rayStrontium markingElectron probe micro-analyzer |
| spellingShingle | Yahua Zhu Tao Jiang Xiubao Chen Hongbo Liu Quinton Phelps Jian Yang Analyses of fin ray types to detect strontium markers in juvenile blunt-snout bream Megalobrama amblycephala Ecological Processes Megalobrama amblycephala Juvenile Fin ray Strontium marking Electron probe micro-analyzer |
| title | Analyses of fin ray types to detect strontium markers in juvenile blunt-snout bream Megalobrama amblycephala |
| title_full | Analyses of fin ray types to detect strontium markers in juvenile blunt-snout bream Megalobrama amblycephala |
| title_fullStr | Analyses of fin ray types to detect strontium markers in juvenile blunt-snout bream Megalobrama amblycephala |
| title_full_unstemmed | Analyses of fin ray types to detect strontium markers in juvenile blunt-snout bream Megalobrama amblycephala |
| title_short | Analyses of fin ray types to detect strontium markers in juvenile blunt-snout bream Megalobrama amblycephala |
| title_sort | analyses of fin ray types to detect strontium markers in juvenile blunt snout bream megalobrama amblycephala |
| topic | Megalobrama amblycephala Juvenile Fin ray Strontium marking Electron probe micro-analyzer |
| url | https://doi.org/10.1186/s13717-024-00501-z |
| work_keys_str_mv | AT yahuazhu analysesoffinraytypestodetectstrontiummarkersinjuvenilebluntsnoutbreammegalobramaamblycephala AT taojiang analysesoffinraytypestodetectstrontiummarkersinjuvenilebluntsnoutbreammegalobramaamblycephala AT xiubaochen analysesoffinraytypestodetectstrontiummarkersinjuvenilebluntsnoutbreammegalobramaamblycephala AT hongboliu analysesoffinraytypestodetectstrontiummarkersinjuvenilebluntsnoutbreammegalobramaamblycephala AT quintonphelps analysesoffinraytypestodetectstrontiummarkersinjuvenilebluntsnoutbreammegalobramaamblycephala AT jianyang analysesoffinraytypestodetectstrontiummarkersinjuvenilebluntsnoutbreammegalobramaamblycephala |