Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity
Summary Toxic polycyclic aromatic hydrocarbons (PAHs) are frequently released into the environment from anthropogenic sources. PAH remediation strategies focus on biological processes mediated by bacteria. The availability of oxygen in polluted environments is often limited or absent, and only bacte...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-11-01
|
| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.12842 |
| _version_ | 1828895201654996992 |
|---|---|
| author | Sophie‐Marie Martirani‐Von Abercron Patricia Marín Marta Solsona‐Ferraz Mayra‐Alejandra Castañeda‐Cataña Silvia Marqués |
| author_facet | Sophie‐Marie Martirani‐Von Abercron Patricia Marín Marta Solsona‐Ferraz Mayra‐Alejandra Castañeda‐Cataña Silvia Marqués |
| author_sort | Sophie‐Marie Martirani‐Von Abercron |
| collection | DOAJ |
| description | Summary Toxic polycyclic aromatic hydrocarbons (PAHs) are frequently released into the environment from anthropogenic sources. PAH remediation strategies focus on biological processes mediated by bacteria. The availability of oxygen in polluted environments is often limited or absent, and only bacteria able to thrive in these conditions can be considered for bioremediation strategies. To identify bacterial strains able to degrade PAHs under oxygen‐limiting conditions, we set up enrichment cultures from samples of an oil‐polluted aquifer, using either anoxic or microaerophilic condition and with PAHs as the sole carbon source. Despite the presence of a significant community of nitrate‐reducing bacteria, the initial community, which was dominated by Betaproteobacteria, was incapable of PAH degradation under strict anoxic conditions, although a clear shift in the structure of the community towards an increase in the Alphaproteobacteria (Sphingomonadaceae), Actinobacteria and an uncultured group of Acidobacteria was observed in the enrichments. In contrast, growth under microaerophilic conditions with naphthalene as the carbon source evidenced the development of a biofilm structure around the naphthalene crystal. The enrichment process selected two co‐dominant groups which finally reached 97% of the bacterial communities: Variovorax spp. (54%, Betaproteobacteria) and Starkeya spp. (43%, Xanthobacteraceae). The two dominant populations were able to grow with naphthalene, although only Starkeya was able to reproduce the biofilm structure around the naphthalene crystal. The pathway for naphthalene degradation was identified, which included as essential steps dioxygenases with high affinity for oxygen, showing 99% identity with Xanthobacter polyaromaticivorans dbd cluster for PAH degradation. Our results suggest that the biofilm formation capacity of Starkeya provided a structure to allocate its cells at an appropriate distance from the toxic carbon source. |
| first_indexed | 2024-12-13T14:24:21Z |
| format | Article |
| id | doaj.art-e256a648f8564b8c876452c380823b4e |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-13T14:24:21Z |
| publishDate | 2017-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-e256a648f8564b8c876452c380823b4e2022-12-21T23:42:00ZengWileyMicrobial Biotechnology1751-79152017-11-011061781179610.1111/1751-7915.12842Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacitySophie‐Marie Martirani‐Von Abercron0Patricia Marín1Marta Solsona‐Ferraz2Mayra‐Alejandra Castañeda‐Cataña3Silvia Marqués4Estación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Granada SpainEstación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Granada SpainEstación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Granada SpainEstación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Granada SpainEstación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Granada SpainSummary Toxic polycyclic aromatic hydrocarbons (PAHs) are frequently released into the environment from anthropogenic sources. PAH remediation strategies focus on biological processes mediated by bacteria. The availability of oxygen in polluted environments is often limited or absent, and only bacteria able to thrive in these conditions can be considered for bioremediation strategies. To identify bacterial strains able to degrade PAHs under oxygen‐limiting conditions, we set up enrichment cultures from samples of an oil‐polluted aquifer, using either anoxic or microaerophilic condition and with PAHs as the sole carbon source. Despite the presence of a significant community of nitrate‐reducing bacteria, the initial community, which was dominated by Betaproteobacteria, was incapable of PAH degradation under strict anoxic conditions, although a clear shift in the structure of the community towards an increase in the Alphaproteobacteria (Sphingomonadaceae), Actinobacteria and an uncultured group of Acidobacteria was observed in the enrichments. In contrast, growth under microaerophilic conditions with naphthalene as the carbon source evidenced the development of a biofilm structure around the naphthalene crystal. The enrichment process selected two co‐dominant groups which finally reached 97% of the bacterial communities: Variovorax spp. (54%, Betaproteobacteria) and Starkeya spp. (43%, Xanthobacteraceae). The two dominant populations were able to grow with naphthalene, although only Starkeya was able to reproduce the biofilm structure around the naphthalene crystal. The pathway for naphthalene degradation was identified, which included as essential steps dioxygenases with high affinity for oxygen, showing 99% identity with Xanthobacter polyaromaticivorans dbd cluster for PAH degradation. Our results suggest that the biofilm formation capacity of Starkeya provided a structure to allocate its cells at an appropriate distance from the toxic carbon source.https://doi.org/10.1111/1751-7915.12842 |
| spellingShingle | Sophie‐Marie Martirani‐Von Abercron Patricia Marín Marta Solsona‐Ferraz Mayra‐Alejandra Castañeda‐Cataña Silvia Marqués Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity Microbial Biotechnology |
| title | Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity |
| title_full | Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity |
| title_fullStr | Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity |
| title_full_unstemmed | Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity |
| title_short | Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity |
| title_sort | naphthalene biodegradation under oxygen limiting conditions community dynamics and the relevance of biofilm forming capacity |
| url | https://doi.org/10.1111/1751-7915.12842 |
| work_keys_str_mv | AT sophiemariemartiranivonabercron naphthalenebiodegradationunderoxygenlimitingconditionscommunitydynamicsandtherelevanceofbiofilmformingcapacity AT patriciamarin naphthalenebiodegradationunderoxygenlimitingconditionscommunitydynamicsandtherelevanceofbiofilmformingcapacity AT martasolsonaferraz naphthalenebiodegradationunderoxygenlimitingconditionscommunitydynamicsandtherelevanceofbiofilmformingcapacity AT mayraalejandracastanedacatana naphthalenebiodegradationunderoxygenlimitingconditionscommunitydynamicsandtherelevanceofbiofilmformingcapacity AT silviamarques naphthalenebiodegradationunderoxygenlimitingconditionscommunitydynamicsandtherelevanceofbiofilmformingcapacity |