<b><i>Iota</i>-carrageenans from <i>Solieria filiformis</i> (Rhodophyta) and their effects in the inflammation and coagulation</b> - doi: 10.4025/actascitechnol.v34i2.11201
<p class="aresumo">Biochemical analyses are important tools for discovering new bioactive compounds for medical clinic. This study aimed at isolating <em>iota</em>-carrageenans (ι-CARs) from <em>Solieria filiformis</em> (Rhodophyta) by enzymatic extraction (EE...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2012-03-01
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| Series: | Acta Scientiarum: Technology |
| Subjects: | |
| Online Access: | http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201 |
| _version_ | 1818569345059520512 |
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| author | Ianna Wivianne Fernandes de Araújo José Ariévilo Gurgel Rodrigues Edfranck de Sousa Oliveira Vanderlei Gabriela Almeida de Paula Ticiana de Brito Lima Norma Maria Barros Benevides |
| author_facet | Ianna Wivianne Fernandes de Araújo José Ariévilo Gurgel Rodrigues Edfranck de Sousa Oliveira Vanderlei Gabriela Almeida de Paula Ticiana de Brito Lima Norma Maria Barros Benevides |
| author_sort | Ianna Wivianne Fernandes de Araújo |
| collection | DOAJ |
| description | <p class="aresumo">Biochemical analyses are important tools for discovering new bioactive compounds for medical clinic. This study aimed at isolating <em>iota</em>-carrageenans (ι-CARs) from <em>Solieria filiformis</em> (Rhodophyta) by enzymatic extraction (EE), refined hot-water extraction (RHWE) and hot-water extraction (HWE), and test (EE, s.c.) their anti-inflammatory effects in the peritonitis model using the Lambda-carrageenan (700 µg cavity<sup>-1</sup>, i.p.) as an inflammatory stimuli in rats. The activated partial thromboplastin time (APTT) was also evaluated in ι-CARs fractions, obtained by ion-exchange chromatography (DEAE-cellulose), using rabbit plasma and compared to heparin (193 IU mg<sup>-1</sup>). The results showed that the ι-CARs (EE) (3 or 9 mg kg<sup>-1</sup>) containing 89.92% total sugars, 29.02% sulfate and absence of contaminant proteins inhibited (p < 0.05) the cellular infiltrate in the peritoneal cavity of the animals, but with 27 mg kg<sup>-1</sup> had no anti-inflammatory effect (p > 0.05). Similar chromatography profiles were obtained among the methods; however, with fractions revealing different pattern on charge density by electrophoresis. Fractions had no virtually effects on APTT (1.16, 1.73 and 1.59 IU mg<sup>-1</sup> for EE, RHWE and HWE, respectively). Further investigations to better understanding the actions of <em>S. filiformis</em> ι-CARs (EE) in the inflammatory response are suggested.</p> |
| first_indexed | 2024-12-14T06:46:54Z |
| format | Article |
| id | doaj.art-e25833fb08894ab3992a188c5b6918d9 |
| institution | Directory Open Access Journal |
| issn | 1806-2563 1807-8664 |
| language | English |
| last_indexed | 2024-12-14T06:46:54Z |
| publishDate | 2012-03-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Technology |
| spelling | doaj.art-e25833fb08894ab3992a188c5b6918d92022-12-21T23:13:00ZengUniversidade Estadual de MaringáActa Scientiarum: Technology1806-25631807-86642012-03-013421271357471<b><i>Iota</i>-carrageenans from <i>Solieria filiformis</i> (Rhodophyta) and their effects in the inflammation and coagulation</b> - doi: 10.4025/actascitechnol.v34i2.11201Ianna Wivianne Fernandes de Araújo0José Ariévilo Gurgel Rodrigues1Edfranck de Sousa Oliveira Vanderlei2Gabriela Almeida de Paula3Ticiana de Brito Lima4Norma Maria Barros Benevides5Universidade Federal do CearáUniversidade Federal do CearáUniversidade Federal do CearáUniversidade Federal do CearáUniversidade Federal do CearáUniversidade Federal do Ceará<p class="aresumo">Biochemical analyses are important tools for discovering new bioactive compounds for medical clinic. This study aimed at isolating <em>iota</em>-carrageenans (ι-CARs) from <em>Solieria filiformis</em> (Rhodophyta) by enzymatic extraction (EE), refined hot-water extraction (RHWE) and hot-water extraction (HWE), and test (EE, s.c.) their anti-inflammatory effects in the peritonitis model using the Lambda-carrageenan (700 µg cavity<sup>-1</sup>, i.p.) as an inflammatory stimuli in rats. The activated partial thromboplastin time (APTT) was also evaluated in ι-CARs fractions, obtained by ion-exchange chromatography (DEAE-cellulose), using rabbit plasma and compared to heparin (193 IU mg<sup>-1</sup>). The results showed that the ι-CARs (EE) (3 or 9 mg kg<sup>-1</sup>) containing 89.92% total sugars, 29.02% sulfate and absence of contaminant proteins inhibited (p < 0.05) the cellular infiltrate in the peritoneal cavity of the animals, but with 27 mg kg<sup>-1</sup> had no anti-inflammatory effect (p > 0.05). Similar chromatography profiles were obtained among the methods; however, with fractions revealing different pattern on charge density by electrophoresis. Fractions had no virtually effects on APTT (1.16, 1.73 and 1.59 IU mg<sup>-1</sup> for EE, RHWE and HWE, respectively). Further investigations to better understanding the actions of <em>S. filiformis</em> ι-CARs (EE) in the inflammatory response are suggested.</p>http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201red algasulfated galactansextraction methodsperitonitisAPTT test |
| spellingShingle | Ianna Wivianne Fernandes de Araújo José Ariévilo Gurgel Rodrigues Edfranck de Sousa Oliveira Vanderlei Gabriela Almeida de Paula Ticiana de Brito Lima Norma Maria Barros Benevides <b><i>Iota</i>-carrageenans from <i>Solieria filiformis</i> (Rhodophyta) and their effects in the inflammation and coagulation</b> - doi: 10.4025/actascitechnol.v34i2.11201 Acta Scientiarum: Technology red alga sulfated galactans extraction methods peritonitis APTT test |
| title | <b><i>Iota</i>-carrageenans from <i>Solieria filiformis</i> (Rhodophyta) and their effects in the inflammation and coagulation</b> - doi: 10.4025/actascitechnol.v34i2.11201 |
| title_full | <b><i>Iota</i>-carrageenans from <i>Solieria filiformis</i> (Rhodophyta) and their effects in the inflammation and coagulation</b> - doi: 10.4025/actascitechnol.v34i2.11201 |
| title_fullStr | <b><i>Iota</i>-carrageenans from <i>Solieria filiformis</i> (Rhodophyta) and their effects in the inflammation and coagulation</b> - doi: 10.4025/actascitechnol.v34i2.11201 |
| title_full_unstemmed | <b><i>Iota</i>-carrageenans from <i>Solieria filiformis</i> (Rhodophyta) and their effects in the inflammation and coagulation</b> - doi: 10.4025/actascitechnol.v34i2.11201 |
| title_short | <b><i>Iota</i>-carrageenans from <i>Solieria filiformis</i> (Rhodophyta) and their effects in the inflammation and coagulation</b> - doi: 10.4025/actascitechnol.v34i2.11201 |
| title_sort | b i iota i carrageenans from i solieria filiformis i rhodophyta and their effects in the inflammation and coagulation b doi 10 4025 actascitechnol v34i2 11201 |
| topic | red alga sulfated galactans extraction methods peritonitis APTT test |
| url | http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201 |
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