Mycobacterium marinum MgtC plays a role in phagocytosis but is dispensable for intracellular multiplication.
MgtC is a virulence factor involved in intramacrophage growth that has been reported in several intracellular pathogens, including Mycobacterium tuberculosis and Salmonella enterica serovar Typhimurium. MgtC participates also in adaptation to Mg2+ deprivation. Herein, we have constructed a mgtC muta...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0116052 |
| _version_ | 1818359654651002880 |
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| author | Claudine Belon Laïla Gannoun-Zaki Georges Lutfalla Laurent Kremer Anne-Béatrice Blanc-Potard |
| author_facet | Claudine Belon Laïla Gannoun-Zaki Georges Lutfalla Laurent Kremer Anne-Béatrice Blanc-Potard |
| author_sort | Claudine Belon |
| collection | DOAJ |
| description | MgtC is a virulence factor involved in intramacrophage growth that has been reported in several intracellular pathogens, including Mycobacterium tuberculosis and Salmonella enterica serovar Typhimurium. MgtC participates also in adaptation to Mg2+ deprivation. Herein, we have constructed a mgtC mutant in Mycobacterium marinum to further investigate the role of MgtC in mycobacteria. We show that the M. marinum mgtC gene (Mma mgtC) is strongly induced upon Mg2+ deprivation and is required for optimal growth in Mg2+-deprived medium. The behaviour of the Mma mgtC mutant has been investigated in the Danio rerio infection model using a transgenic reporter zebrafish line that specifically labels neutrophils. Although the mgtC mutant is not attenuated in the zebrafish embryo model based on survival curves, our results indicate that phagocytosis by neutrophils is enhanced with the mgtC mutant compared to the wild-type strain following subcutaneous injection. Increased phagocytosis of the mutant strain is also observed ex vivo with the murine J774 macrophage cell line. On the other hand, no difference was found between the mgtC mutant and the wild-type strain in bacterial adhesion to macrophages and in the internalization into epithelial cells. Unlike the role reported for MgtC in other intracellular pathogens, Mma MgtC does not contribute significantly to intramacrophage replication. Taken together, these results indicate an unanticipated function of Mma MgtC at early step of infection within phagocytic cells. Hence, our results indicate that although the MgtC function is conserved among pathogens regarding adaptation to Mg2+ deprivation, its role towards phagocytic cells can differ, possibly in relation with the specific pathogen's lifestyles. |
| first_indexed | 2024-12-13T20:48:20Z |
| format | Article |
| id | doaj.art-e25ac279a8ba4d508f14fdc10f80911a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T20:48:20Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e25ac279a8ba4d508f14fdc10f80911a2022-12-21T23:31:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11605210.1371/journal.pone.0116052Mycobacterium marinum MgtC plays a role in phagocytosis but is dispensable for intracellular multiplication.Claudine BelonLaïla Gannoun-ZakiGeorges LutfallaLaurent KremerAnne-Béatrice Blanc-PotardMgtC is a virulence factor involved in intramacrophage growth that has been reported in several intracellular pathogens, including Mycobacterium tuberculosis and Salmonella enterica serovar Typhimurium. MgtC participates also in adaptation to Mg2+ deprivation. Herein, we have constructed a mgtC mutant in Mycobacterium marinum to further investigate the role of MgtC in mycobacteria. We show that the M. marinum mgtC gene (Mma mgtC) is strongly induced upon Mg2+ deprivation and is required for optimal growth in Mg2+-deprived medium. The behaviour of the Mma mgtC mutant has been investigated in the Danio rerio infection model using a transgenic reporter zebrafish line that specifically labels neutrophils. Although the mgtC mutant is not attenuated in the zebrafish embryo model based on survival curves, our results indicate that phagocytosis by neutrophils is enhanced with the mgtC mutant compared to the wild-type strain following subcutaneous injection. Increased phagocytosis of the mutant strain is also observed ex vivo with the murine J774 macrophage cell line. On the other hand, no difference was found between the mgtC mutant and the wild-type strain in bacterial adhesion to macrophages and in the internalization into epithelial cells. Unlike the role reported for MgtC in other intracellular pathogens, Mma MgtC does not contribute significantly to intramacrophage replication. Taken together, these results indicate an unanticipated function of Mma MgtC at early step of infection within phagocytic cells. Hence, our results indicate that although the MgtC function is conserved among pathogens regarding adaptation to Mg2+ deprivation, its role towards phagocytic cells can differ, possibly in relation with the specific pathogen's lifestyles.https://doi.org/10.1371/journal.pone.0116052 |
| spellingShingle | Claudine Belon Laïla Gannoun-Zaki Georges Lutfalla Laurent Kremer Anne-Béatrice Blanc-Potard Mycobacterium marinum MgtC plays a role in phagocytosis but is dispensable for intracellular multiplication. PLoS ONE |
| title | Mycobacterium marinum MgtC plays a role in phagocytosis but is dispensable for intracellular multiplication. |
| title_full | Mycobacterium marinum MgtC plays a role in phagocytosis but is dispensable for intracellular multiplication. |
| title_fullStr | Mycobacterium marinum MgtC plays a role in phagocytosis but is dispensable for intracellular multiplication. |
| title_full_unstemmed | Mycobacterium marinum MgtC plays a role in phagocytosis but is dispensable for intracellular multiplication. |
| title_short | Mycobacterium marinum MgtC plays a role in phagocytosis but is dispensable for intracellular multiplication. |
| title_sort | mycobacterium marinum mgtc plays a role in phagocytosis but is dispensable for intracellular multiplication |
| url | https://doi.org/10.1371/journal.pone.0116052 |
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