Evaluation of <i>Pyrenophora tritici-repentis</i> Infection of Wheat Heads
The incidence of wheat head infection by <i>Pyrenophora tritici-repentis</i> (Ptr), the etiological agent of tan spot disease, was evaluated during grain development in a glasshouse experiment. Heads artificially inoculated with a Ptr spore suspension developed widespread brown spots acr...
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MDPI AG
2020-09-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/10/9/417 |
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| author | Pao Theen See Nikki Schultz Caroline S. Moffat |
| author_facet | Pao Theen See Nikki Schultz Caroline S. Moffat |
| author_sort | Pao Theen See |
| collection | DOAJ |
| description | The incidence of wheat head infection by <i>Pyrenophora tritici-repentis</i> (Ptr), the etiological agent of tan spot disease, was evaluated during grain development in a glasshouse experiment. Heads artificially inoculated with a Ptr spore suspension developed widespread brown spots across the spikelets, and mycelia and conidophores were observed on glumes and awns. Seeds of heavily infected heads were darkened and shrivelled, but no red smudge symptoms were apparent. The recovery rate of Ptr isolates from the inoculated wheat heads was low, and colonies that were re-isolated displayed an irregular morphology with reddish mycelia when grown on agar plates. The presence of Ptr on inoculated wheat heads was assessed directly via PCR detection, and a limitation of Ptr hyphae to proliferate beyond the point of contact of spore inoculum on floret tissues was observed. The systemic transmission of Ptr from infected seeds was minimal; however, saprophytic growth of the pathogen occurred on the senescing leaves of wheat plants grown from inoculated seeds. Thus, even though Ptr seed infection is not as common as foliar infection, infected seeds are still a source of disease inoculum and screening for pathogen contamination is advisable. |
| first_indexed | 2024-03-10T16:11:08Z |
| format | Article |
| id | doaj.art-e25b09adc77946b288bcbec318f851fc |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T16:11:08Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj.art-e25b09adc77946b288bcbec318f851fc2023-11-20T14:30:49ZengMDPI AGAgriculture2077-04722020-09-0110941710.3390/agriculture10090417Evaluation of <i>Pyrenophora tritici-repentis</i> Infection of Wheat HeadsPao Theen See0Nikki Schultz1Caroline S. Moffat2Centre for Crop and Disease Management, School of Molecular Life Sciences, Curtin University, Bentley, Western Australia 6102, AustraliaCentre for Crop and Disease Management, School of Molecular Life Sciences, Curtin University, Bentley, Western Australia 6102, AustraliaCentre for Crop and Disease Management, School of Molecular Life Sciences, Curtin University, Bentley, Western Australia 6102, AustraliaThe incidence of wheat head infection by <i>Pyrenophora tritici-repentis</i> (Ptr), the etiological agent of tan spot disease, was evaluated during grain development in a glasshouse experiment. Heads artificially inoculated with a Ptr spore suspension developed widespread brown spots across the spikelets, and mycelia and conidophores were observed on glumes and awns. Seeds of heavily infected heads were darkened and shrivelled, but no red smudge symptoms were apparent. The recovery rate of Ptr isolates from the inoculated wheat heads was low, and colonies that were re-isolated displayed an irregular morphology with reddish mycelia when grown on agar plates. The presence of Ptr on inoculated wheat heads was assessed directly via PCR detection, and a limitation of Ptr hyphae to proliferate beyond the point of contact of spore inoculum on floret tissues was observed. The systemic transmission of Ptr from infected seeds was minimal; however, saprophytic growth of the pathogen occurred on the senescing leaves of wheat plants grown from inoculated seeds. Thus, even though Ptr seed infection is not as common as foliar infection, infected seeds are still a source of disease inoculum and screening for pathogen contamination is advisable.https://www.mdpi.com/2077-0472/10/9/417tan spotyellow spotnecrotrophwheatplant pathogen |
| spellingShingle | Pao Theen See Nikki Schultz Caroline S. Moffat Evaluation of <i>Pyrenophora tritici-repentis</i> Infection of Wheat Heads Agriculture tan spot yellow spot necrotroph wheat plant pathogen |
| title | Evaluation of <i>Pyrenophora tritici-repentis</i> Infection of Wheat Heads |
| title_full | Evaluation of <i>Pyrenophora tritici-repentis</i> Infection of Wheat Heads |
| title_fullStr | Evaluation of <i>Pyrenophora tritici-repentis</i> Infection of Wheat Heads |
| title_full_unstemmed | Evaluation of <i>Pyrenophora tritici-repentis</i> Infection of Wheat Heads |
| title_short | Evaluation of <i>Pyrenophora tritici-repentis</i> Infection of Wheat Heads |
| title_sort | evaluation of i pyrenophora tritici repentis i infection of wheat heads |
| topic | tan spot yellow spot necrotroph wheat plant pathogen |
| url | https://www.mdpi.com/2077-0472/10/9/417 |
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