Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.
Epstein Barr virus (EBV) infection expands CD8+ T cells specific for lytic antigens to high frequencies during symptomatic primary infection, and maintains these at significant numbers during persistence. Despite this, the protective function of these lytic EBV antigen-specific cytotoxic CD8+ T cell...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-08-01
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| Series: | PLoS Pathogens |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25165855/?tool=EBI |
| _version_ | 1819320602724401152 |
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| author | Olga Antsiferova Anne Müller Patrick C Rämer Obinna Chijioke Bithi Chatterjee Ana Raykova Raquel Planas Mireia Sospedra Anatoliy Shumilov Ming-Han Tsai Henri-Jacques Delecluse Christian Münz |
| author_facet | Olga Antsiferova Anne Müller Patrick C Rämer Obinna Chijioke Bithi Chatterjee Ana Raykova Raquel Planas Mireia Sospedra Anatoliy Shumilov Ming-Han Tsai Henri-Jacques Delecluse Christian Münz |
| author_sort | Olga Antsiferova |
| collection | DOAJ |
| description | Epstein Barr virus (EBV) infection expands CD8+ T cells specific for lytic antigens to high frequencies during symptomatic primary infection, and maintains these at significant numbers during persistence. Despite this, the protective function of these lytic EBV antigen-specific cytotoxic CD8+ T cells remains unclear. Here we demonstrate that lytic EBV replication does not significantly contribute to virus-induced B cell proliferation in vitro and in vivo in a mouse model with reconstituted human immune system components (huNSG mice). However, we report a trend to reduction of EBV-induced lymphoproliferation outside of lymphoid organs upon diminished lytic replication. Moreover, we could demonstrate that CD8+ T cells against the lytic EBV antigen BMLF1 can eliminate lytically replicating EBV-transformed B cells from lymphoblastoid cell lines (LCLs) and in vivo, thereby transiently controlling high viremia after adoptive transfer into EBV infected huNSG mice. These findings suggest a protective function for lytic EBV antigen-specific CD8+ T cells against EBV infection and against virus-associated tumors in extra-lymphoid organs. These specificities should be explored for EBV-specific vaccine development. |
| first_indexed | 2024-12-24T11:22:12Z |
| format | Article |
| id | doaj.art-e25caa9499d24c2d95619727227fe222 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-24T11:22:12Z |
| publishDate | 2014-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-e25caa9499d24c2d95619727227fe2222022-12-21T16:58:12ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742014-08-01108e100433310.1371/journal.ppat.1004333Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.Olga AntsiferovaAnne MüllerPatrick C RämerObinna ChijiokeBithi ChatterjeeAna RaykovaRaquel PlanasMireia SospedraAnatoliy ShumilovMing-Han TsaiHenri-Jacques DelecluseChristian MünzEpstein Barr virus (EBV) infection expands CD8+ T cells specific for lytic antigens to high frequencies during symptomatic primary infection, and maintains these at significant numbers during persistence. Despite this, the protective function of these lytic EBV antigen-specific cytotoxic CD8+ T cells remains unclear. Here we demonstrate that lytic EBV replication does not significantly contribute to virus-induced B cell proliferation in vitro and in vivo in a mouse model with reconstituted human immune system components (huNSG mice). However, we report a trend to reduction of EBV-induced lymphoproliferation outside of lymphoid organs upon diminished lytic replication. Moreover, we could demonstrate that CD8+ T cells against the lytic EBV antigen BMLF1 can eliminate lytically replicating EBV-transformed B cells from lymphoblastoid cell lines (LCLs) and in vivo, thereby transiently controlling high viremia after adoptive transfer into EBV infected huNSG mice. These findings suggest a protective function for lytic EBV antigen-specific CD8+ T cells against EBV infection and against virus-associated tumors in extra-lymphoid organs. These specificities should be explored for EBV-specific vaccine development.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25165855/?tool=EBI |
| spellingShingle | Olga Antsiferova Anne Müller Patrick C Rämer Obinna Chijioke Bithi Chatterjee Ana Raykova Raquel Planas Mireia Sospedra Anatoliy Shumilov Ming-Han Tsai Henri-Jacques Delecluse Christian Münz Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. PLoS Pathogens |
| title | Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. |
| title_full | Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. |
| title_fullStr | Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. |
| title_full_unstemmed | Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. |
| title_short | Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. |
| title_sort | adoptive transfer of ebv specific cd8 t cell clones can transiently control ebv infection in humanized mice |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25165855/?tool=EBI |
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