Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia
Food products may be a source of <i>Salmonella</i>, one of the main causal agents of food poisoning, especially after the emergence of strains resistant to antimicrobial preparations. The present work dealt with investigation of the occurrence of resistance to antimicrobial preparations...
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2021-12-01
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author | Andrey L. Rakitin Yulia K. Yushina Elena V. Zaiko Dagmara S. Bataeva Oksana A. Kuznetsova Anastasia A. Semenova Svetlana A. Ermolaeva Aleksey V. Beletskiy Tat’yana V. Kolganova Andrey V. Mardanov Sergei O. Shapovalov Timofey E. Tkachik |
author_facet | Andrey L. Rakitin Yulia K. Yushina Elena V. Zaiko Dagmara S. Bataeva Oksana A. Kuznetsova Anastasia A. Semenova Svetlana A. Ermolaeva Aleksey V. Beletskiy Tat’yana V. Kolganova Andrey V. Mardanov Sergei O. Shapovalov Timofey E. Tkachik |
author_sort | Andrey L. Rakitin |
collection | DOAJ |
description | Food products may be a source of <i>Salmonella</i>, one of the main causal agents of food poisoning, especially after the emergence of strains resistant to antimicrobial preparations. The present work dealt with investigation of the occurrence of resistance to antimicrobial preparations among <i>S. enterica</i> strains isolated from food. The isolates belonged to 11 serovars, among which Infantis (28%), Enteritidis (19%), and Typhimurium (13.4%) predominated. The isolates were most commonly resistant to trimethoprim/sulfamethoxazole (<i>n =</i> 19, 59.38%), cefazolin (<i>n =</i> 15, 46.86%), tetracycline (<i>n =</i> 13, 40.63%), and amikacin (<i>n =</i> 9, 28.13%). Most of the strains (68.75%) exhibited multiple resistance to commonly used antibiotics. High-throughput sequencing was used to analyse three multidrug-resistant strains (resistant to six or more antibiotics). Two of them (SZL 30 and SZL 31) belonged to <i>S.</i> Infantis, while one strain belonged to <i>S.</i> Typhimurium (SZL 38). Analysis of the genomes of the sequenced strains revealed the genes responsible for antibiotic resistance. In the genomes of strains SZL 30 and SZL 31 the genes of antibiotic resistance were shown to be localized mostly in integrons within plasmids, while most of the antibiotic resistance genes of strain SZL 38 were localized in a chromosomal island (17,949 nt). Genomes of the <i>Salmonella</i> strains SZL 30, SZL 31, and SZL 38 were shown to contain full-size pathogenicity islands: SPI-1, SPI-2, SPI-4, SPI-5, SPI-9, SPI-11, SPI-13, SPI-14, and CS54. Moreover, the genome of strain SZL 38 was also found to contain the full-size pathogenicity islands SPI-3, SPI-6, SPI-12, and SPI-16. The emergence of multidrug-resistant strains of various <i>Salmonella</i> serovars indicates that further research on the transmission pathways for these genetic determinants and monitoring of the distribution of these microorganisms are necessary. |
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spelling | doaj.art-e25dcc6b970947ff82ec3d85405aa0b12022-12-22T04:05:43ZengMDPI AGAntibiotics2079-63822021-12-01111110.3390/antibiotics11010001Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in RussiaAndrey L. Rakitin0Yulia K. Yushina1Elena V. Zaiko2Dagmara S. Bataeva3Oksana A. Kuznetsova4Anastasia A. Semenova5Svetlana A. Ermolaeva6Aleksey V. Beletskiy7Tat’yana V. Kolganova8Andrey V. Mardanov9Sergei O. Shapovalov10Timofey E. Tkachik11Research Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, 119071 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaFederal Research Center for Virology and Microbiology, Nizhny Novgorod Research Veterinary Institute Branch, 603950 Nizhny Novgorod, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, 119071 Moscow, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, 119071 Moscow, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, 119071 Moscow, RussiaResearch and Scientific Testing Center “Cherkizovo”, 108805 Moscow, RussiaResearch and Scientific Testing Center “Cherkizovo”, 108805 Moscow, RussiaFood products may be a source of <i>Salmonella</i>, one of the main causal agents of food poisoning, especially after the emergence of strains resistant to antimicrobial preparations. The present work dealt with investigation of the occurrence of resistance to antimicrobial preparations among <i>S. enterica</i> strains isolated from food. The isolates belonged to 11 serovars, among which Infantis (28%), Enteritidis (19%), and Typhimurium (13.4%) predominated. The isolates were most commonly resistant to trimethoprim/sulfamethoxazole (<i>n =</i> 19, 59.38%), cefazolin (<i>n =</i> 15, 46.86%), tetracycline (<i>n =</i> 13, 40.63%), and amikacin (<i>n =</i> 9, 28.13%). Most of the strains (68.75%) exhibited multiple resistance to commonly used antibiotics. High-throughput sequencing was used to analyse three multidrug-resistant strains (resistant to six or more antibiotics). Two of them (SZL 30 and SZL 31) belonged to <i>S.</i> Infantis, while one strain belonged to <i>S.</i> Typhimurium (SZL 38). Analysis of the genomes of the sequenced strains revealed the genes responsible for antibiotic resistance. In the genomes of strains SZL 30 and SZL 31 the genes of antibiotic resistance were shown to be localized mostly in integrons within plasmids, while most of the antibiotic resistance genes of strain SZL 38 were localized in a chromosomal island (17,949 nt). Genomes of the <i>Salmonella</i> strains SZL 30, SZL 31, and SZL 38 were shown to contain full-size pathogenicity islands: SPI-1, SPI-2, SPI-4, SPI-5, SPI-9, SPI-11, SPI-13, SPI-14, and CS54. Moreover, the genome of strain SZL 38 was also found to contain the full-size pathogenicity islands SPI-3, SPI-6, SPI-12, and SPI-16. The emergence of multidrug-resistant strains of various <i>Salmonella</i> serovars indicates that further research on the transmission pathways for these genetic determinants and monitoring of the distribution of these microorganisms are necessary.https://www.mdpi.com/2079-6382/11/1/1<i>Salmonella</i>foodborne pathogenserotypeWGSantimicrobial resistance |
spellingShingle | Andrey L. Rakitin Yulia K. Yushina Elena V. Zaiko Dagmara S. Bataeva Oksana A. Kuznetsova Anastasia A. Semenova Svetlana A. Ermolaeva Aleksey V. Beletskiy Tat’yana V. Kolganova Andrey V. Mardanov Sergei O. Shapovalov Timofey E. Tkachik Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia Antibiotics <i>Salmonella</i> foodborne pathogen serotype WGS antimicrobial resistance |
title | Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia |
title_full | Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia |
title_fullStr | Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia |
title_full_unstemmed | Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia |
title_short | Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia |
title_sort | evaluation of antibiotic resistance of i salmonella i serotypes and whole genome sequencing of multiresistant strains isolated from food products in russia |
topic | <i>Salmonella</i> foodborne pathogen serotype WGS antimicrobial resistance |
url | https://www.mdpi.com/2079-6382/11/1/1 |
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