Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia

Food products may be a source of <i>Salmonella</i>, one of the main causal agents of food poisoning, especially after the emergence of strains resistant to antimicrobial preparations. The present work dealt with investigation of the occurrence of resistance to antimicrobial preparations...

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Main Authors: Andrey L. Rakitin, Yulia K. Yushina, Elena V. Zaiko, Dagmara S. Bataeva, Oksana A. Kuznetsova, Anastasia A. Semenova, Svetlana A. Ermolaeva, Aleksey V. Beletskiy, Tat’yana V. Kolganova, Andrey V. Mardanov, Sergei O. Shapovalov, Timofey E. Tkachik
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Antibiotics
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Online Access:https://www.mdpi.com/2079-6382/11/1/1
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author Andrey L. Rakitin
Yulia K. Yushina
Elena V. Zaiko
Dagmara S. Bataeva
Oksana A. Kuznetsova
Anastasia A. Semenova
Svetlana A. Ermolaeva
Aleksey V. Beletskiy
Tat’yana V. Kolganova
Andrey V. Mardanov
Sergei O. Shapovalov
Timofey E. Tkachik
author_facet Andrey L. Rakitin
Yulia K. Yushina
Elena V. Zaiko
Dagmara S. Bataeva
Oksana A. Kuznetsova
Anastasia A. Semenova
Svetlana A. Ermolaeva
Aleksey V. Beletskiy
Tat’yana V. Kolganova
Andrey V. Mardanov
Sergei O. Shapovalov
Timofey E. Tkachik
author_sort Andrey L. Rakitin
collection DOAJ
description Food products may be a source of <i>Salmonella</i>, one of the main causal agents of food poisoning, especially after the emergence of strains resistant to antimicrobial preparations. The present work dealt with investigation of the occurrence of resistance to antimicrobial preparations among <i>S. enterica</i> strains isolated from food. The isolates belonged to 11 serovars, among which Infantis (28%), Enteritidis (19%), and Typhimurium (13.4%) predominated. The isolates were most commonly resistant to trimethoprim/sulfamethoxazole (<i>n =</i> 19, 59.38%), cefazolin (<i>n =</i> 15, 46.86%), tetracycline (<i>n =</i> 13, 40.63%), and amikacin (<i>n =</i> 9, 28.13%). Most of the strains (68.75%) exhibited multiple resistance to commonly used antibiotics. High-throughput sequencing was used to analyse three multidrug-resistant strains (resistant to six or more antibiotics). Two of them (SZL 30 and SZL 31) belonged to <i>S.</i> Infantis, while one strain belonged to <i>S.</i> Typhimurium (SZL 38). Analysis of the genomes of the sequenced strains revealed the genes responsible for antibiotic resistance. In the genomes of strains SZL 30 and SZL 31 the genes of antibiotic resistance were shown to be localized mostly in integrons within plasmids, while most of the antibiotic resistance genes of strain SZL 38 were localized in a chromosomal island (17,949 nt). Genomes of the <i>Salmonella</i> strains SZL 30, SZL 31, and SZL 38 were shown to contain full-size pathogenicity islands: SPI-1, SPI-2, SPI-4, SPI-5, SPI-9, SPI-11, SPI-13, SPI-14, and CS54. Moreover, the genome of strain SZL 38 was also found to contain the full-size pathogenicity islands SPI-3, SPI-6, SPI-12, and SPI-16. The emergence of multidrug-resistant strains of various <i>Salmonella</i> serovars indicates that further research on the transmission pathways for these genetic determinants and monitoring of the distribution of these microorganisms are necessary.
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spelling doaj.art-e25dcc6b970947ff82ec3d85405aa0b12022-12-22T04:05:43ZengMDPI AGAntibiotics2079-63822021-12-01111110.3390/antibiotics11010001Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in RussiaAndrey L. Rakitin0Yulia K. Yushina1Elena V. Zaiko2Dagmara S. Bataeva3Oksana A. Kuznetsova4Anastasia A. Semenova5Svetlana A. Ermolaeva6Aleksey V. Beletskiy7Tat’yana V. Kolganova8Andrey V. Mardanov9Sergei O. Shapovalov10Timofey E. Tkachik11Research Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, 119071 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, RussiaFederal Research Center for Virology and Microbiology, Nizhny Novgorod Research Veterinary Institute Branch, 603950 Nizhny Novgorod, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, 119071 Moscow, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, 119071 Moscow, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, 119071 Moscow, RussiaResearch and Scientific Testing Center “Cherkizovo”, 108805 Moscow, RussiaResearch and Scientific Testing Center “Cherkizovo”, 108805 Moscow, RussiaFood products may be a source of <i>Salmonella</i>, one of the main causal agents of food poisoning, especially after the emergence of strains resistant to antimicrobial preparations. The present work dealt with investigation of the occurrence of resistance to antimicrobial preparations among <i>S. enterica</i> strains isolated from food. The isolates belonged to 11 serovars, among which Infantis (28%), Enteritidis (19%), and Typhimurium (13.4%) predominated. The isolates were most commonly resistant to trimethoprim/sulfamethoxazole (<i>n =</i> 19, 59.38%), cefazolin (<i>n =</i> 15, 46.86%), tetracycline (<i>n =</i> 13, 40.63%), and amikacin (<i>n =</i> 9, 28.13%). Most of the strains (68.75%) exhibited multiple resistance to commonly used antibiotics. High-throughput sequencing was used to analyse three multidrug-resistant strains (resistant to six or more antibiotics). Two of them (SZL 30 and SZL 31) belonged to <i>S.</i> Infantis, while one strain belonged to <i>S.</i> Typhimurium (SZL 38). Analysis of the genomes of the sequenced strains revealed the genes responsible for antibiotic resistance. In the genomes of strains SZL 30 and SZL 31 the genes of antibiotic resistance were shown to be localized mostly in integrons within plasmids, while most of the antibiotic resistance genes of strain SZL 38 were localized in a chromosomal island (17,949 nt). Genomes of the <i>Salmonella</i> strains SZL 30, SZL 31, and SZL 38 were shown to contain full-size pathogenicity islands: SPI-1, SPI-2, SPI-4, SPI-5, SPI-9, SPI-11, SPI-13, SPI-14, and CS54. Moreover, the genome of strain SZL 38 was also found to contain the full-size pathogenicity islands SPI-3, SPI-6, SPI-12, and SPI-16. The emergence of multidrug-resistant strains of various <i>Salmonella</i> serovars indicates that further research on the transmission pathways for these genetic determinants and monitoring of the distribution of these microorganisms are necessary.https://www.mdpi.com/2079-6382/11/1/1<i>Salmonella</i>foodborne pathogenserotypeWGSantimicrobial resistance
spellingShingle Andrey L. Rakitin
Yulia K. Yushina
Elena V. Zaiko
Dagmara S. Bataeva
Oksana A. Kuznetsova
Anastasia A. Semenova
Svetlana A. Ermolaeva
Aleksey V. Beletskiy
Tat’yana V. Kolganova
Andrey V. Mardanov
Sergei O. Shapovalov
Timofey E. Tkachik
Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia
Antibiotics
<i>Salmonella</i>
foodborne pathogen
serotype
WGS
antimicrobial resistance
title Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia
title_full Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia
title_fullStr Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia
title_full_unstemmed Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia
title_short Evaluation of Antibiotic Resistance of <i>Salmonella</i> Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia
title_sort evaluation of antibiotic resistance of i salmonella i serotypes and whole genome sequencing of multiresistant strains isolated from food products in russia
topic <i>Salmonella</i>
foodborne pathogen
serotype
WGS
antimicrobial resistance
url https://www.mdpi.com/2079-6382/11/1/1
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