The effects of gestational and lactational exposure to Nonylphenol on c-jun, and c-fos expression and learning and memory in hippocampus of male F1 rat
Objective(s): To investigate the effects of Nonylphenol (NP) in pups from dams exposed during gestational and lactational periods on immediate early genes (c-jun, c-fos) in hippocampus and the learning and memory of F1 rats. Materials and Methods: Twenty eight pregnant dams, stratified by pregnancy...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2017-04-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_8578_d37a05c44b6e1fe541166ec70bfd5c4a.pdf |
| _version_ | 1811244845692354560 |
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| author | Yu Jie Wang Pan Yan Wenxia Gao Feng He Liting Li Wenmei Xu Jie |
| author_facet | Yu Jie Wang Pan Yan Wenxia Gao Feng He Liting Li Wenmei Xu Jie |
| author_sort | Yu Jie |
| collection | DOAJ |
| description | Objective(s): To investigate the effects of Nonylphenol (NP) in pups from dams exposed during gestational and lactational periods on immediate early genes (c-jun, c-fos) in hippocampus and the learning and memory of F1 rats. Materials and Methods: Twenty eight pregnant dams, stratified by pregnancy date, were randomly assigned into 4 groups, which were gavaged with NP at the doses of 50 mg/kg/day, 100 mg/kg/day, 200 mg/kg/day and groundnut oil, respectively. Step-down avoidance test, and learning and memory effects of NP were evaluated on 8-weeks-old pups. The expressions of c-jun and c-fos and the activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AchE) were evaluated in hippocampus of pups. Results: Compared to the control, reaction time (RT) that pups spent to jump to the platform was longer (P=0.02), the number of errors were higher (P=0.01), and the step-down latency was shorter in the 200 mg/kg/day NP-treated group (P=0.04). Exposure to NP induced a significant reduction in ChAT activity in hippocampus in the 100 (P=0.005) and 200 mg/kg/day NP-treated groups (P=0.002), whereas exposure to 200 mg/kg/day caused a significant increase in AchE activity (P=0.004); a dose–response relationship was revealed between ChAT and AchE activities and NP exposure in the hippocampus of pups (r=-0.821, P=0.01; r=0.757, P=0.04). Exposure to NP in the 100 and 200 mg/kg/day NP-treated groups exhibited an increase in number of c-fos and c-jun positive cells. Conclusion: Exposure to NP might negatively affect learning and memory ability in F1 rats, possibly due to the alterations in the expression of c-jun and c-fos, and ChAT, AchE activities in hippocampus of pups. |
| first_indexed | 2024-04-12T14:31:54Z |
| format | Article |
| id | doaj.art-e25df57c5f394c2895fe0db03d4b52dc |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-12T14:31:54Z |
| publishDate | 2017-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-e25df57c5f394c2895fe0db03d4b52dc2022-12-22T03:29:16ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742017-04-0120438639110.22038/ijbms.2017.85788578The effects of gestational and lactational exposure to Nonylphenol on c-jun, and c-fos expression and learning and memory in hippocampus of male F1 ratYu Jie0Wang Pan1Yan Wenxia2Gao Feng3He Liting4Li Wenmei5Xu Jie6School of Public Health, Zunyi Medical University, Zunyi Guizhou 563099, P.R. of ChinaDepartment of Nuclear Medicine, The Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou 563099, P.R. of ChinaSchool of Public Health, Zunyi Medical University, Zunyi Guizhou 563099, P.R. of ChinaSchool of Public Health, Zunyi Medical University, Zunyi Guizhou 563099, P.R. of ChinaSchool of Public Health, Zunyi Medical University, Zunyi Guizhou 563099, P.R. of ChinaSchool of Public Health, Zunyi Medical University, Zunyi Guizhou 563099, P.R. of ChinaSchool of Public Health, Zunyi Medical University, Zunyi Guizhou 563099, P.R. of ChinaObjective(s): To investigate the effects of Nonylphenol (NP) in pups from dams exposed during gestational and lactational periods on immediate early genes (c-jun, c-fos) in hippocampus and the learning and memory of F1 rats. Materials and Methods: Twenty eight pregnant dams, stratified by pregnancy date, were randomly assigned into 4 groups, which were gavaged with NP at the doses of 50 mg/kg/day, 100 mg/kg/day, 200 mg/kg/day and groundnut oil, respectively. Step-down avoidance test, and learning and memory effects of NP were evaluated on 8-weeks-old pups. The expressions of c-jun and c-fos and the activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AchE) were evaluated in hippocampus of pups. Results: Compared to the control, reaction time (RT) that pups spent to jump to the platform was longer (P=0.02), the number of errors were higher (P=0.01), and the step-down latency was shorter in the 200 mg/kg/day NP-treated group (P=0.04). Exposure to NP induced a significant reduction in ChAT activity in hippocampus in the 100 (P=0.005) and 200 mg/kg/day NP-treated groups (P=0.002), whereas exposure to 200 mg/kg/day caused a significant increase in AchE activity (P=0.004); a dose–response relationship was revealed between ChAT and AchE activities and NP exposure in the hippocampus of pups (r=-0.821, P=0.01; r=0.757, P=0.04). Exposure to NP in the 100 and 200 mg/kg/day NP-treated groups exhibited an increase in number of c-fos and c-jun positive cells. Conclusion: Exposure to NP might negatively affect learning and memory ability in F1 rats, possibly due to the alterations in the expression of c-jun and c-fos, and ChAT, AchE activities in hippocampus of pups.http://ijbms.mums.ac.ir/article_8578_d37a05c44b6e1fe541166ec70bfd5c4a.pdfLearning and memoryNeurotransmitters NonylphenolPups |
| spellingShingle | Yu Jie Wang Pan Yan Wenxia Gao Feng He Liting Li Wenmei Xu Jie The effects of gestational and lactational exposure to Nonylphenol on c-jun, and c-fos expression and learning and memory in hippocampus of male F1 rat Iranian Journal of Basic Medical Sciences Learning and memory Neurotransmitters Nonylphenol Pups |
| title | The effects of gestational and lactational exposure to Nonylphenol on c-jun, and c-fos expression and learning and memory in hippocampus of male F1 rat |
| title_full | The effects of gestational and lactational exposure to Nonylphenol on c-jun, and c-fos expression and learning and memory in hippocampus of male F1 rat |
| title_fullStr | The effects of gestational and lactational exposure to Nonylphenol on c-jun, and c-fos expression and learning and memory in hippocampus of male F1 rat |
| title_full_unstemmed | The effects of gestational and lactational exposure to Nonylphenol on c-jun, and c-fos expression and learning and memory in hippocampus of male F1 rat |
| title_short | The effects of gestational and lactational exposure to Nonylphenol on c-jun, and c-fos expression and learning and memory in hippocampus of male F1 rat |
| title_sort | effects of gestational and lactational exposure to nonylphenol on c jun and c fos expression and learning and memory in hippocampus of male f1 rat |
| topic | Learning and memory Neurotransmitters Nonylphenol Pups |
| url | http://ijbms.mums.ac.ir/article_8578_d37a05c44b6e1fe541166ec70bfd5c4a.pdf |
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