DNA Barcoding Identifies Unknown Females and Larvae of <i>Fannia</i> R.-D. (Diptera: Fanniidae) from Carrion Succession Experiment and Case Report
Application of available keys to European Fanniidae did not facilitate unequivocal species identification for third instar larvae and females of <i>Fannia</i> Robineau-Desvoidy, 1830 collected during a study of arthropod succession on pig carrion. To link these samples to known species,...
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MDPI AG
2021-04-01
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| author | Andrzej Grzywacz Mateusz Jarmusz Kinga Walczak Rafał Skowronek Nikolas P. Johnston Krzysztof Szpila |
| author_facet | Andrzej Grzywacz Mateusz Jarmusz Kinga Walczak Rafał Skowronek Nikolas P. Johnston Krzysztof Szpila |
| author_sort | Andrzej Grzywacz |
| collection | DOAJ |
| description | Application of available keys to European Fanniidae did not facilitate unequivocal species identification for third instar larvae and females of <i>Fannia</i> Robineau-Desvoidy, 1830 collected during a study of arthropod succession on pig carrion. To link these samples to known species, we took the advantage of molecular identification methods and compared newly obtained cytochrome oxidase subunit I (COI) barcode sequences against sequences deposited in reference databases. As an outcome of the results obtained, we describe for the first time a third instar larva of <i>Fannia nigra</i> Malloch, 1910 and <i>Fannia pallitibia</i> (Rondani, 1866) and a female of <i>Fannia collini</i> d’Assis-Fonseca, 1966. We provide combinations of characters allowing for discrimination of described insects from other Fanniidae. We provide an update for the key by Rozkošný et al. 1997, which allows differentiation between females of <i>F. collini</i> and other species of Fanniidae. Additionally, we provide a case of a human cadaver discovered in Southern Poland and insect fauna associated with it as the first report of <i>F. nigra</i> larvae developing on a human body. |
| first_indexed | 2024-03-10T12:03:16Z |
| format | Article |
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| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T12:03:16Z |
| publishDate | 2021-04-01 |
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| record_format | Article |
| series | Insects |
| spelling | doaj.art-e2603bcaaf46490a88eac4469d2a02542023-11-21T16:48:27ZengMDPI AGInsects2075-44502021-04-0112538110.3390/insects12050381DNA Barcoding Identifies Unknown Females and Larvae of <i>Fannia</i> R.-D. (Diptera: Fanniidae) from Carrion Succession Experiment and Case ReportAndrzej Grzywacz0Mateusz Jarmusz1Kinga Walczak2Rafał Skowronek3Nikolas P. Johnston4Krzysztof Szpila5Department of Ecology and Biogeography, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, 87-100 Toruń, PolandDepartment of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University in Poznań, 61-712 Poznań, PolandDepartment of Ecology and Biogeography, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, 87-100 Toruń, PolandDepartment of Forensic Medicine and Forensic Toxicology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, 40-055 Katowice, PolandDepartment of Ecology and Biogeography, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, 87-100 Toruń, PolandDepartment of Ecology and Biogeography, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, 87-100 Toruń, PolandApplication of available keys to European Fanniidae did not facilitate unequivocal species identification for third instar larvae and females of <i>Fannia</i> Robineau-Desvoidy, 1830 collected during a study of arthropod succession on pig carrion. To link these samples to known species, we took the advantage of molecular identification methods and compared newly obtained cytochrome oxidase subunit I (COI) barcode sequences against sequences deposited in reference databases. As an outcome of the results obtained, we describe for the first time a third instar larva of <i>Fannia nigra</i> Malloch, 1910 and <i>Fannia pallitibia</i> (Rondani, 1866) and a female of <i>Fannia collini</i> d’Assis-Fonseca, 1966. We provide combinations of characters allowing for discrimination of described insects from other Fanniidae. We provide an update for the key by Rozkošný et al. 1997, which allows differentiation between females of <i>F. collini</i> and other species of Fanniidae. Additionally, we provide a case of a human cadaver discovered in Southern Poland and insect fauna associated with it as the first report of <i>F. nigra</i> larvae developing on a human body.https://www.mdpi.com/2075-4450/12/5/381Fanniidaelarval morphologyforensic entomologyhuman cadaver |
| spellingShingle | Andrzej Grzywacz Mateusz Jarmusz Kinga Walczak Rafał Skowronek Nikolas P. Johnston Krzysztof Szpila DNA Barcoding Identifies Unknown Females and Larvae of <i>Fannia</i> R.-D. (Diptera: Fanniidae) from Carrion Succession Experiment and Case Report Insects Fanniidae larval morphology forensic entomology human cadaver |
| title | DNA Barcoding Identifies Unknown Females and Larvae of <i>Fannia</i> R.-D. (Diptera: Fanniidae) from Carrion Succession Experiment and Case Report |
| title_full | DNA Barcoding Identifies Unknown Females and Larvae of <i>Fannia</i> R.-D. (Diptera: Fanniidae) from Carrion Succession Experiment and Case Report |
| title_fullStr | DNA Barcoding Identifies Unknown Females and Larvae of <i>Fannia</i> R.-D. (Diptera: Fanniidae) from Carrion Succession Experiment and Case Report |
| title_full_unstemmed | DNA Barcoding Identifies Unknown Females and Larvae of <i>Fannia</i> R.-D. (Diptera: Fanniidae) from Carrion Succession Experiment and Case Report |
| title_short | DNA Barcoding Identifies Unknown Females and Larvae of <i>Fannia</i> R.-D. (Diptera: Fanniidae) from Carrion Succession Experiment and Case Report |
| title_sort | dna barcoding identifies unknown females and larvae of i fannia i r d diptera fanniidae from carrion succession experiment and case report |
| topic | Fanniidae larval morphology forensic entomology human cadaver |
| url | https://www.mdpi.com/2075-4450/12/5/381 |
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