Prevalence and genetic diversity of <em>Grapevine virus A</em> in Tunisia

Prevalence and genetic diversity of the complete CP gene of Grapevine virus A (GVA) were assessed in isolates from rootstocks, wine and table grape varieties and autochthonous grapevines. Wine grapes were the most infected (63%), followed by table grapes (49%) and rootstocks (44%). Autochthonous gra...

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Main Authors: Ilhem SELMI, Arezki LEHAD, Davide PACIFICO, Francesco CARIMI, Naima Mahfoudhi
Format: Article
Language:English
Published: Firenze University Press 2018-09-01
Series:Phytopathologia Mediterranea
Subjects:
Online Access:https://oajournals.fupress.net/index.php/pm/article/view/5763
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author Ilhem SELMI
Arezki LEHAD
Davide PACIFICO
Francesco CARIMI
Naima Mahfoudhi
author_facet Ilhem SELMI
Arezki LEHAD
Davide PACIFICO
Francesco CARIMI
Naima Mahfoudhi
author_sort Ilhem SELMI
collection DOAJ
description Prevalence and genetic diversity of the complete CP gene of Grapevine virus A (GVA) were assessed in isolates from rootstocks, wine and table grape varieties and autochthonous grapevines. Wine grapes were the most infected (63%), followed by table grapes (49%) and rootstocks (44%). Autochthonous grapevines were the less infected (35%). Analyses of the complete coat protein sequences of 20 GVA isolates from the main grapevine growing areas of Tunisia identified three phylogroups, accounting, respectively, for 70% (group I), 25% (group IV) and 5% (group III) of the isolates. No sequences clustered into group II. Phylogenetic analyses indicated that Tunisian GVA isolates are not grouped by the host cultivar or geographic origin.
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spelling doaj.art-e260d45f365a4726b9a4ae0151b2e3832022-12-22T03:28:13ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952018-09-0157210.14601/Phytopathol_Mediterr-22123Prevalence and genetic diversity of <em>Grapevine virus A</em> in TunisiaIlhem SELMI0Arezki LEHAD1Davide PACIFICO2Francesco CARIMI3Naima Mahfoudhi4Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 1004 ElMenzah, Tunis, TunisieLaboratoire de Phytopathologie et Biologie Moléculaire. Ecole Nationale Supérieure d’Agronomie, Rue Hacen Badi, Belfort, El Harrach, 16000 Alger, AlgérieIstituto di Bioscienze e BioRisorse (IBBR), Consiglio Nazionale delle Ricerche- CNR Corso Calatafimi 414, 90129 Palermo, ItaliaIstituto di Bioscienze e BioRisorse (IBBR), Consiglio Nazionale delle Ricerche- CNR Corso Calatafimi 414, 90129 Palermo, ItaliaLaboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 1004 ElMenzah, Tunis, TunisiePrevalence and genetic diversity of the complete CP gene of Grapevine virus A (GVA) were assessed in isolates from rootstocks, wine and table grape varieties and autochthonous grapevines. Wine grapes were the most infected (63%), followed by table grapes (49%) and rootstocks (44%). Autochthonous grapevines were the less infected (35%). Analyses of the complete coat protein sequences of 20 GVA isolates from the main grapevine growing areas of Tunisia identified three phylogroups, accounting, respectively, for 70% (group I), 25% (group IV) and 5% (group III) of the isolates. No sequences clustered into group II. Phylogenetic analyses indicated that Tunisian GVA isolates are not grouped by the host cultivar or geographic origin.https://oajournals.fupress.net/index.php/pm/article/view/5763grapevineGVAmolecular analysisdiversity
spellingShingle Ilhem SELMI
Arezki LEHAD
Davide PACIFICO
Francesco CARIMI
Naima Mahfoudhi
Prevalence and genetic diversity of <em>Grapevine virus A</em> in Tunisia
Phytopathologia Mediterranea
grapevine
GVA
molecular analysis
diversity
title Prevalence and genetic diversity of <em>Grapevine virus A</em> in Tunisia
title_full Prevalence and genetic diversity of <em>Grapevine virus A</em> in Tunisia
title_fullStr Prevalence and genetic diversity of <em>Grapevine virus A</em> in Tunisia
title_full_unstemmed Prevalence and genetic diversity of <em>Grapevine virus A</em> in Tunisia
title_short Prevalence and genetic diversity of <em>Grapevine virus A</em> in Tunisia
title_sort prevalence and genetic diversity of em grapevine virus a em in tunisia
topic grapevine
GVA
molecular analysis
diversity
url https://oajournals.fupress.net/index.php/pm/article/view/5763
work_keys_str_mv AT ilhemselmi prevalenceandgeneticdiversityofemgrapevinevirusaemintunisia
AT arezkilehad prevalenceandgeneticdiversityofemgrapevinevirusaemintunisia
AT davidepacifico prevalenceandgeneticdiversityofemgrapevinevirusaemintunisia
AT francescocarimi prevalenceandgeneticdiversityofemgrapevinevirusaemintunisia
AT naimamahfoudhi prevalenceandgeneticdiversityofemgrapevinevirusaemintunisia