SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β
Abstract Superparamagnetic iron oxide nanoparticles (SPIO) have been synthesized and explored for use as carriers of various nanoadjuvants via loading into dendritic cells (DCs). In our study, homogeneous and superparamagnetic nanoparticles are susceptible to internalization by DCs and SPIO-pulsed D...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2018-12-01
|
| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11671-018-2802-0 |
| _version_ | 1797708555617304576 |
|---|---|
| author | Hui Liu Heng Dong Na Zhou Shiling Dong Lin Chen Yanxiang Zhu Hong-ming Hu Yongbin Mou |
| author_facet | Hui Liu Heng Dong Na Zhou Shiling Dong Lin Chen Yanxiang Zhu Hong-ming Hu Yongbin Mou |
| author_sort | Hui Liu |
| collection | DOAJ |
| description | Abstract Superparamagnetic iron oxide nanoparticles (SPIO) have been synthesized and explored for use as carriers of various nanoadjuvants via loading into dendritic cells (DCs). In our study, homogeneous and superparamagnetic nanoparticles are susceptible to internalization by DCs and SPIO-pulsed DCs showed excellent biocompatibility and capacity for ovalbumin (OVA) cross-presentation. Herein, we found that SPIO-loaded DCs can promote the maturation and migration of DCs in vitro. SPIO coated with 3-aminopropyltrimethoxysilane (APTS) and meso-2,3-dimercaptosuccinic acid (DMSA), which present positive and negative charges, respectively, were prepared. We aimed to investigate whether the surface charge of SPIO can affect the antigen cross-presentation of the DCs. Additionally, the formation of interleukin-1β (IL-1β) was examined after treatment with oppositely charged SPIO to identify the nanoadjuvants mechanism. In conclusion, our results suggest that SPIO are biocompatible and can induce the migration of DCs into secondary lymph nodes. SPIO coated with APTS (SPIO/A+) exhibited excellent adjuvant potentials for the promotion of antigen cross-presentation and T cell activation and surpassed that of DMSA-coated nanoparticles (SPIO/D−). This process may be related to the secretion of IL-1β. Our study provides insights into the predictive modification of nanoadjuvants, which will be valuable in DC vaccine design and could lead to the creation of new adjuvants for applications in vaccines for humans. |
| first_indexed | 2024-03-12T06:24:24Z |
| format | Article |
| id | doaj.art-e262b8ae02234d6daff3886506102669 |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T06:24:24Z |
| publishDate | 2018-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-e262b8ae02234d6daff38865061026692023-09-03T02:04:11ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2018-12-0113111110.1186/s11671-018-2802-0SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1βHui Liu0Heng Dong1Na Zhou2Shiling Dong3Lin Chen4Yanxiang Zhu5Hong-ming Hu6Yongbin Mou7Central Laboratory, Nanjing Stomatological Hospital, Medical School of Nanjing UniversityCentral Laboratory, Nanjing Stomatological Hospital, Medical School of Nanjing UniversityCentral Laboratory, Nanjing Stomatological Hospital, Medical School of Nanjing UniversityCentral Laboratory, Nanjing Stomatological Hospital, Medical School of Nanjing UniversityCentral Laboratory, Nanjing Stomatological Hospital, Medical School of Nanjing UniversityCentral Laboratory, Nanjing Stomatological Hospital, Medical School of Nanjing UniversityLaboratory of Cancer Immunobiology, Robert W. Franz Cancer Research Center, Earle A. Chiles Research Institute, Providence Cancer CenterCentral Laboratory, Nanjing Stomatological Hospital, Medical School of Nanjing UniversityAbstract Superparamagnetic iron oxide nanoparticles (SPIO) have been synthesized and explored for use as carriers of various nanoadjuvants via loading into dendritic cells (DCs). In our study, homogeneous and superparamagnetic nanoparticles are susceptible to internalization by DCs and SPIO-pulsed DCs showed excellent biocompatibility and capacity for ovalbumin (OVA) cross-presentation. Herein, we found that SPIO-loaded DCs can promote the maturation and migration of DCs in vitro. SPIO coated with 3-aminopropyltrimethoxysilane (APTS) and meso-2,3-dimercaptosuccinic acid (DMSA), which present positive and negative charges, respectively, were prepared. We aimed to investigate whether the surface charge of SPIO can affect the antigen cross-presentation of the DCs. Additionally, the formation of interleukin-1β (IL-1β) was examined after treatment with oppositely charged SPIO to identify the nanoadjuvants mechanism. In conclusion, our results suggest that SPIO are biocompatible and can induce the migration of DCs into secondary lymph nodes. SPIO coated with APTS (SPIO/A+) exhibited excellent adjuvant potentials for the promotion of antigen cross-presentation and T cell activation and surpassed that of DMSA-coated nanoparticles (SPIO/D−). This process may be related to the secretion of IL-1β. Our study provides insights into the predictive modification of nanoadjuvants, which will be valuable in DC vaccine design and could lead to the creation of new adjuvants for applications in vaccines for humans.http://link.springer.com/article/10.1186/s11671-018-2802-0Superparamagnetic iron oxide nanoparticlesDendritic cellsCross-presentationSurface chargeInterleukin-1βNanoadjuvant |
| spellingShingle | Hui Liu Heng Dong Na Zhou Shiling Dong Lin Chen Yanxiang Zhu Hong-ming Hu Yongbin Mou SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β Nanoscale Research Letters Superparamagnetic iron oxide nanoparticles Dendritic cells Cross-presentation Surface charge Interleukin-1β Nanoadjuvant |
| title | SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β |
| title_full | SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β |
| title_fullStr | SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β |
| title_full_unstemmed | SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β |
| title_short | SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β |
| title_sort | spio enhance the cross presentation and migration of dcs and anionic spio influence the nanoadjuvant effects related to interleukin 1β |
| topic | Superparamagnetic iron oxide nanoparticles Dendritic cells Cross-presentation Surface charge Interleukin-1β Nanoadjuvant |
| url | http://link.springer.com/article/10.1186/s11671-018-2802-0 |
| work_keys_str_mv | AT huiliu spioenhancethecrosspresentationandmigrationofdcsandanionicspioinfluencethenanoadjuvanteffectsrelatedtointerleukin1b AT hengdong spioenhancethecrosspresentationandmigrationofdcsandanionicspioinfluencethenanoadjuvanteffectsrelatedtointerleukin1b AT nazhou spioenhancethecrosspresentationandmigrationofdcsandanionicspioinfluencethenanoadjuvanteffectsrelatedtointerleukin1b AT shilingdong spioenhancethecrosspresentationandmigrationofdcsandanionicspioinfluencethenanoadjuvanteffectsrelatedtointerleukin1b AT linchen spioenhancethecrosspresentationandmigrationofdcsandanionicspioinfluencethenanoadjuvanteffectsrelatedtointerleukin1b AT yanxiangzhu spioenhancethecrosspresentationandmigrationofdcsandanionicspioinfluencethenanoadjuvanteffectsrelatedtointerleukin1b AT hongminghu spioenhancethecrosspresentationandmigrationofdcsandanionicspioinfluencethenanoadjuvanteffectsrelatedtointerleukin1b AT yongbinmou spioenhancethecrosspresentationandmigrationofdcsandanionicspioinfluencethenanoadjuvanteffectsrelatedtointerleukin1b |