In vitro fermentation of κ-carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on HT29 cells
In vitro fermental behaviors and colonic inflammation of κ-carrageenan oligosaccharides (KO3 and KO6) obtained from simulated gastric digestion were investigated. With the proceeding of fermentation, κ-carrageenan oligosaccharides were readily degraded, exerting a notable impact on production of SCF...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-08-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464619302932 |
| _version_ | 1818723688982249472 |
|---|---|
| author | Yujiao Sun Xiangyi Cui Mengmeng Duan Chunqing Ai Shuang Song Xuefeng Chen |
| author_facet | Yujiao Sun Xiangyi Cui Mengmeng Duan Chunqing Ai Shuang Song Xuefeng Chen |
| author_sort | Yujiao Sun |
| collection | DOAJ |
| description | In vitro fermental behaviors and colonic inflammation of κ-carrageenan oligosaccharides (KO3 and KO6) obtained from simulated gastric digestion were investigated. With the proceeding of fermentation, κ-carrageenan oligosaccharides were readily degraded, exerting a notable impact on production of SCFAs and composition of gut microbiota. Both KO3 and KO6 significantly promoted pro-inflammatory bacteria Prevotella, while inhibited anti-inflammatory bacteria Bacteroides and Parabacteroides. Notably, fermental behavior of KO3 with larger degrees of polymerization (DPs) and KO6 with smaller DPs showed different responsible for fermentation, of which KO3 improved SCFAs production and highly increased the growth of Bifidobacterium and Lactobacillius, while KO6 reduced SCFAs production and enormously enhanced the abundance of mucin-producing bacteria Prevotellaceae. Associated with inflammatory effect on HT29 cells, KO6 fermental supernatants more promoted the secretions of IL-1β, TNF-α, SIgA and mucin2, hinting that smaller κ-carrageenan oligosaccharides might pose stronger inflammation. The results can be helpful for understanding the safety of κ-carrageenan oligosaccharides. |
| first_indexed | 2024-12-17T21:14:30Z |
| format | Article |
| id | doaj.art-e262efe7edd945dbb0a647e81ec7e7e9 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T21:14:30Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e262efe7edd945dbb0a647e81ec7e7e92022-12-21T21:32:23ZengElsevierJournal of Functional Foods1756-46462019-08-01598091In vitro fermentation of κ-carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on HT29 cellsYujiao Sun0Xiangyi Cui1Mengmeng Duan2Chunqing Ai3Shuang Song4Xuefeng Chen5Natural Food Macromolecule Research Center, School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, PR China; School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China; Corresponding author.Natural Food Macromolecule Research Center, School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, PR ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR ChinaNatural Food Macromolecule Research Center, School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, PR ChinaIn vitro fermental behaviors and colonic inflammation of κ-carrageenan oligosaccharides (KO3 and KO6) obtained from simulated gastric digestion were investigated. With the proceeding of fermentation, κ-carrageenan oligosaccharides were readily degraded, exerting a notable impact on production of SCFAs and composition of gut microbiota. Both KO3 and KO6 significantly promoted pro-inflammatory bacteria Prevotella, while inhibited anti-inflammatory bacteria Bacteroides and Parabacteroides. Notably, fermental behavior of KO3 with larger degrees of polymerization (DPs) and KO6 with smaller DPs showed different responsible for fermentation, of which KO3 improved SCFAs production and highly increased the growth of Bifidobacterium and Lactobacillius, while KO6 reduced SCFAs production and enormously enhanced the abundance of mucin-producing bacteria Prevotellaceae. Associated with inflammatory effect on HT29 cells, KO6 fermental supernatants more promoted the secretions of IL-1β, TNF-α, SIgA and mucin2, hinting that smaller κ-carrageenan oligosaccharides might pose stronger inflammation. The results can be helpful for understanding the safety of κ-carrageenan oligosaccharides.http://www.sciencedirect.com/science/article/pii/S1756464619302932κ-Carrageenan oligosaccharidesFermentationGut microbiotaInflammation |
| spellingShingle | Yujiao Sun Xiangyi Cui Mengmeng Duan Chunqing Ai Shuang Song Xuefeng Chen In vitro fermentation of κ-carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on HT29 cells Journal of Functional Foods κ-Carrageenan oligosaccharides Fermentation Gut microbiota Inflammation |
| title | In vitro fermentation of κ-carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on HT29 cells |
| title_full | In vitro fermentation of κ-carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on HT29 cells |
| title_fullStr | In vitro fermentation of κ-carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on HT29 cells |
| title_full_unstemmed | In vitro fermentation of κ-carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on HT29 cells |
| title_short | In vitro fermentation of κ-carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on HT29 cells |
| title_sort | in vitro fermentation of κ carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on ht29 cells |
| topic | κ-Carrageenan oligosaccharides Fermentation Gut microbiota Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S1756464619302932 |
| work_keys_str_mv | AT yujiaosun invitrofermentationofkcarrageenanoligosaccharidesbyhumangutmicrobiotaanditsinflammatoryeffectonht29cells AT xiangyicui invitrofermentationofkcarrageenanoligosaccharidesbyhumangutmicrobiotaanditsinflammatoryeffectonht29cells AT mengmengduan invitrofermentationofkcarrageenanoligosaccharidesbyhumangutmicrobiotaanditsinflammatoryeffectonht29cells AT chunqingai invitrofermentationofkcarrageenanoligosaccharidesbyhumangutmicrobiotaanditsinflammatoryeffectonht29cells AT shuangsong invitrofermentationofkcarrageenanoligosaccharidesbyhumangutmicrobiotaanditsinflammatoryeffectonht29cells AT xuefengchen invitrofermentationofkcarrageenanoligosaccharidesbyhumangutmicrobiotaanditsinflammatoryeffectonht29cells |