In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension
Summary: Artificial cells can shed new light on the molecular basis for life and hold potential for new chemical technologies. Inspired by how nature dynamically regulates its membrane compartments, we aim to repurpose the endosomal sorting complex required for transport (ESCRT) to generate complex...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-05-01
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| Series: | iScience |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900421930118X |
| _version_ | 1811282112532185088 |
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| author | Andrew Booth Christopher J. Marklew Barbara Ciani Paul A. Beales |
| author_facet | Andrew Booth Christopher J. Marklew Barbara Ciani Paul A. Beales |
| author_sort | Andrew Booth |
| collection | DOAJ |
| description | Summary: Artificial cells can shed new light on the molecular basis for life and hold potential for new chemical technologies. Inspired by how nature dynamically regulates its membrane compartments, we aim to repurpose the endosomal sorting complex required for transport (ESCRT) to generate complex membrane architectures as suitable scaffolds for artificial cells. Purified ESCRT-III components perform topological transformations on giant unilamellar vesicles to create complex “vesicles-within-a-vesicle” architectures resembling the compartmentalization in eukaryotic cells. Thus far, the proposed mechanisms for this activity are based on how assembly and disassembly of ESCRT-III on the membrane drives deformation. Here we demonstrate the existence of a negative feedback mechanism from membrane mechanics that regulates ESCRT-III remodeling activity. Intraluminal vesicle (ILV) formation removes excess membrane area, increasing tension, which in turn suppresses downstream ILV formation. This mechanism for in vitro regulation of ESCRT-III activity may also have important implications for its in vivo functions. : Biochemistry; Bioengineering; Cell Biology; Biophysics Subject Areas: Biochemistry, Bioengineering, Cell Biology, Biophysics |
| first_indexed | 2024-04-13T01:45:46Z |
| format | Article |
| id | doaj.art-e264cc031f0d49cb8cde9be9055b535b |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-13T01:45:46Z |
| publishDate | 2019-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-e264cc031f0d49cb8cde9be9055b535b2022-12-22T03:08:02ZengElsevieriScience2589-00422019-05-0115173184In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane TensionAndrew Booth0Christopher J. Marklew1Barbara Ciani2Paul A. Beales3School of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UKDepartment of Chemistry and Centre for Membrane Interactions and Dynamics, University of Sheffield, Sheffield S3 7HF, UKDepartment of Chemistry and Centre for Membrane Interactions and Dynamics, University of Sheffield, Sheffield S3 7HF, UK; Corresponding authorSchool of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK; Corresponding authorSummary: Artificial cells can shed new light on the molecular basis for life and hold potential for new chemical technologies. Inspired by how nature dynamically regulates its membrane compartments, we aim to repurpose the endosomal sorting complex required for transport (ESCRT) to generate complex membrane architectures as suitable scaffolds for artificial cells. Purified ESCRT-III components perform topological transformations on giant unilamellar vesicles to create complex “vesicles-within-a-vesicle” architectures resembling the compartmentalization in eukaryotic cells. Thus far, the proposed mechanisms for this activity are based on how assembly and disassembly of ESCRT-III on the membrane drives deformation. Here we demonstrate the existence of a negative feedback mechanism from membrane mechanics that regulates ESCRT-III remodeling activity. Intraluminal vesicle (ILV) formation removes excess membrane area, increasing tension, which in turn suppresses downstream ILV formation. This mechanism for in vitro regulation of ESCRT-III activity may also have important implications for its in vivo functions. : Biochemistry; Bioengineering; Cell Biology; Biophysics Subject Areas: Biochemistry, Bioengineering, Cell Biology, Biophysicshttp://www.sciencedirect.com/science/article/pii/S258900421930118X |
| spellingShingle | Andrew Booth Christopher J. Marklew Barbara Ciani Paul A. Beales In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension iScience |
| title | In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension |
| title_full | In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension |
| title_fullStr | In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension |
| title_full_unstemmed | In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension |
| title_short | In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension |
| title_sort | in vitro membrane remodeling by escrt is regulated by negative feedback from membrane tension |
| url | http://www.sciencedirect.com/science/article/pii/S258900421930118X |
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