First Case of Actinomycetoma in France Due to a Novel <italic toggle="yes">Nocardia</italic> Species, <italic toggle="yes">Nocardia boironii</italic> sp. nov
ABSTRACT Bacterial mycetoma is a neglected disease mainly observed in tropical area countries and typically associated with rural conditions, making its presence in developed countries of temperate climate areas rare. However, we report the first case of an autochthonous mycetoma case in continental...
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American Society for Microbiology
2016-12-01
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| Series: | mSphere |
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| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00309-16 |
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| author | Jacques M. Gilquin Brigitte Riviere Valme Jurado Bernard Audouy Jean-Baptiste Kouatche Emmanuelle Bergeron Delphine Mouniée Thierry Molina Philippe Faure Cesáreo Saiz-Jimenez Verónica Rodríguez-Nava |
| author_facet | Jacques M. Gilquin Brigitte Riviere Valme Jurado Bernard Audouy Jean-Baptiste Kouatche Emmanuelle Bergeron Delphine Mouniée Thierry Molina Philippe Faure Cesáreo Saiz-Jimenez Verónica Rodríguez-Nava |
| author_sort | Jacques M. Gilquin |
| collection | DOAJ |
| description | ABSTRACT Bacterial mycetoma is a neglected disease mainly observed in tropical area countries and typically associated with rural conditions, making its presence in developed countries of temperate climate areas rare. However, we report the first case of an autochthonous mycetoma case in continental France that originated from a new Nocardia species. A Gram-positive filamentous bacterium (OFN 14.177T) was isolated from a pus sample from the mycetoma of a male French patient 92 years old suffering from chronic lymphocytic leukemia. The isolate was analyzed by a polyphasic taxonomic approach by coupling morphological, biochemical, physiological, and chemotaxonomic aspects to genomic and phylogenetic analyses. Multilocus sequence analysis (MLSA) using four housekeeping genes (16S rRNA gene, secA1, hsp65, and sod) combined with phylogenetic analysis revealed that the strain OFN 14.177T is phylogenetically closer not only to Nocardia altamirensis but also to all other species comprising the Nocardia brasiliensis clade (i.e., N. brasiliensis, N. altamirensis, N. vulneris, N. iowensis, and N. tenerifensis), some of which present cutaneous tropism. The G+C content of isolate OFN 14.177T was 68.2 mol%. DNA-DNA hybridization analyses demonstrated 38.25% relative reassociation with N. altamirensis. The strain OFN 14.177T is different from the closest species at genetic and phenotypical levels, and the data obtained indicate that it should be recognized as a new species, for which the name of Nocardia boironii sp. nov. is proposed. The type strain is OFN 14.177T (= EML 1451 = DSM 101696). IMPORTANCE Bacterial mycetoma is an endemic infection in areas with tropical and subtropical climates. Thus, its presence in temperate climate areas remains rare. We report here the first case of autochthonous actinomycetoma in continental France originating from a Nocardia species other than N. brasiliensis, namely, Nocardia boironii. Considering the history of the patient, the infection source of strain OFN 14.177T may be from frequent contact with the soil over many years because of his gardening activities. The discovery of a French autochthonous Nocardia species responsible for actinomycetoma reveals the importance of considering the possibility of having autochthonous infections of this type in nontropical countries, not only imported cases from tropical countries. However, further studies are needed to elucidate the real incidence of this new species. |
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| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-14T06:25:46Z |
| publishDate | 2016-12-01 |
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| spelling | doaj.art-e265b59b604a46b789a20b6dbd04f0a12022-12-21T23:13:41ZengAmerican Society for MicrobiologymSphere2379-50422016-12-011610.1128/mSphere.00309-16First Case of Actinomycetoma in France Due to a Novel <italic toggle="yes">Nocardia</italic> Species, <italic toggle="yes">Nocardia boironii</italic> sp. novJacques M. Gilquin0Brigitte Riviere1Valme Jurado2Bernard Audouy3Jean-Baptiste Kouatche4Emmanuelle Bergeron5Delphine Mouniée6Thierry Molina7Philippe Faure8Cesáreo Saiz-Jimenez9Verónica Rodríguez-Nava10Service de Médecine Interne, Centre Hospitalier de Castres-Mazamet, Castres, FranceLaboratoire de Microbiologie, Centre Hospitalier de Castres-Mazamet, Castres, FranceInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Seville, SpainService d’Imagerie Médicale, Centre Hospitalier de Castres-Mazamet, Castres, FranceResearch Group on Bacterial Opportunistic Pathogens and Environment, UMR 5557, Ecologie Microbienne, French Observatory of Nocardiosis, Université de Lyon, Université de Lyon, CNRS, VetAgro Sup, Lyon, FranceResearch Group on Bacterial Opportunistic Pathogens and Environment, UMR 5557, Ecologie Microbienne, French Observatory of Nocardiosis, Université de Lyon, Université de Lyon, CNRS, VetAgro Sup, Lyon, FranceResearch Group on Bacterial Opportunistic Pathogens and Environment, UMR 5557, Ecologie Microbienne, French Observatory of Nocardiosis, Université de Lyon, Université de Lyon, CNRS, VetAgro Sup, Lyon, FranceService d’Anatomie et de Cytologie Pathologiques, Hôpital Necker Enfants Malades, Paris, FranceLaboratoire d'Anatomie et de Cytologie Pathologiques, Castres, FranceInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Seville, SpainResearch Group on Bacterial Opportunistic Pathogens and Environment, UMR 5557, Ecologie Microbienne, French Observatory of Nocardiosis, Université de Lyon, Université de Lyon, CNRS, VetAgro Sup, Lyon, FranceABSTRACT Bacterial mycetoma is a neglected disease mainly observed in tropical area countries and typically associated with rural conditions, making its presence in developed countries of temperate climate areas rare. However, we report the first case of an autochthonous mycetoma case in continental France that originated from a new Nocardia species. A Gram-positive filamentous bacterium (OFN 14.177T) was isolated from a pus sample from the mycetoma of a male French patient 92 years old suffering from chronic lymphocytic leukemia. The isolate was analyzed by a polyphasic taxonomic approach by coupling morphological, biochemical, physiological, and chemotaxonomic aspects to genomic and phylogenetic analyses. Multilocus sequence analysis (MLSA) using four housekeeping genes (16S rRNA gene, secA1, hsp65, and sod) combined with phylogenetic analysis revealed that the strain OFN 14.177T is phylogenetically closer not only to Nocardia altamirensis but also to all other species comprising the Nocardia brasiliensis clade (i.e., N. brasiliensis, N. altamirensis, N. vulneris, N. iowensis, and N. tenerifensis), some of which present cutaneous tropism. The G+C content of isolate OFN 14.177T was 68.2 mol%. DNA-DNA hybridization analyses demonstrated 38.25% relative reassociation with N. altamirensis. The strain OFN 14.177T is different from the closest species at genetic and phenotypical levels, and the data obtained indicate that it should be recognized as a new species, for which the name of Nocardia boironii sp. nov. is proposed. The type strain is OFN 14.177T (= EML 1451 = DSM 101696). IMPORTANCE Bacterial mycetoma is an endemic infection in areas with tropical and subtropical climates. Thus, its presence in temperate climate areas remains rare. We report here the first case of autochthonous actinomycetoma in continental France originating from a Nocardia species other than N. brasiliensis, namely, Nocardia boironii. Considering the history of the patient, the infection source of strain OFN 14.177T may be from frequent contact with the soil over many years because of his gardening activities. The discovery of a French autochthonous Nocardia species responsible for actinomycetoma reveals the importance of considering the possibility of having autochthonous infections of this type in nontropical countries, not only imported cases from tropical countries. However, further studies are needed to elucidate the real incidence of this new species.https://journals.asm.org/doi/10.1128/mSphere.00309-16MLSANocardiaantibiotic resistancegenotypic identificationmycetomaphenotypic identification |
| spellingShingle | Jacques M. Gilquin Brigitte Riviere Valme Jurado Bernard Audouy Jean-Baptiste Kouatche Emmanuelle Bergeron Delphine Mouniée Thierry Molina Philippe Faure Cesáreo Saiz-Jimenez Verónica Rodríguez-Nava First Case of Actinomycetoma in France Due to a Novel <italic toggle="yes">Nocardia</italic> Species, <italic toggle="yes">Nocardia boironii</italic> sp. nov mSphere MLSA Nocardia antibiotic resistance genotypic identification mycetoma phenotypic identification |
| title | First Case of Actinomycetoma in France Due to a Novel <italic toggle="yes">Nocardia</italic> Species, <italic toggle="yes">Nocardia boironii</italic> sp. nov |
| title_full | First Case of Actinomycetoma in France Due to a Novel <italic toggle="yes">Nocardia</italic> Species, <italic toggle="yes">Nocardia boironii</italic> sp. nov |
| title_fullStr | First Case of Actinomycetoma in France Due to a Novel <italic toggle="yes">Nocardia</italic> Species, <italic toggle="yes">Nocardia boironii</italic> sp. nov |
| title_full_unstemmed | First Case of Actinomycetoma in France Due to a Novel <italic toggle="yes">Nocardia</italic> Species, <italic toggle="yes">Nocardia boironii</italic> sp. nov |
| title_short | First Case of Actinomycetoma in France Due to a Novel <italic toggle="yes">Nocardia</italic> Species, <italic toggle="yes">Nocardia boironii</italic> sp. nov |
| title_sort | first case of actinomycetoma in france due to a novel italic toggle yes nocardia italic species italic toggle yes nocardia boironii italic sp nov |
| topic | MLSA Nocardia antibiotic resistance genotypic identification mycetoma phenotypic identification |
| url | https://journals.asm.org/doi/10.1128/mSphere.00309-16 |
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