Experimental and computational workflow for the analysis of tRNA pools from eukaryotic cells by mim-tRNAseq

Experimental and computational workflow for the analysis of tRNA pools from eukaryotic cells by mim-tRNAseq

Summary: Quantifying tRNAs is crucial for understanding how they regulate mRNA translation but is hampered by their extensive sequence similarity and premature termination of reverse transcription at multiple modified nucleotides. Here, we describe the use of modification-induced misincorporation tR...

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Bibliographic Details
Main Authors:	Andrew Behrens, Danny D. Nedialkova
Format:	Article
Language:	English
Published:	Elsevier 2022-09-01
Series:	STAR Protocols
Subjects:	Bioinformatics Sequencing RNAseq Molecular Biology Gene Expression Systems biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722004592

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