Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate
The deteriorating function of the kidneys in chronic kidney disease (CKD) is associated, among other things, with the retention of many unnecessary metabolic products in the body. Indoxyl sulfate (IS) belongs to the group of uremic toxins with a high protein binding affinity. Moreover, this compound...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/12/3848 |
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| author | Anna Pieniazek Michal Kopera Lukasz Gwozdzinski Krzysztof Gwozdzinski |
| author_facet | Anna Pieniazek Michal Kopera Lukasz Gwozdzinski Krzysztof Gwozdzinski |
| author_sort | Anna Pieniazek |
| collection | DOAJ |
| description | The deteriorating function of the kidneys in chronic kidney disease (CKD) is associated, among other things, with the retention of many unnecessary metabolic products in the body. Indoxyl sulfate (IS) belongs to the group of uremic toxins with a high protein binding affinity. Moreover, this compound can generate oxidative stress. We hypothesized that a high concentration of IS might induce oxidative changes in erythrocytes and plasma components, and could therefore contribute to CKD progression. In this study, we evaluated the influence of IS on the oxidative stress parameters in plasma and hemolysate. Moreover, as a result of the action of IS, we observed a decrease in the total antioxidant capacity and a change in the activity of catalase and superoxide dismutase in hemolysate and plasma. The obtained results indicate that IS induces oxidative damage to hemolysate and plasma components. Greater changes in the parameters of oxidative stress were observed in hemolysate than in plasma treated with indoxyl sulfate. The obtained results suggest that the increased concentration of IS in patients with chronic kidney disease may lead to a decrease in the lifespan of erythrocytes in their bloodstream. |
| first_indexed | 2024-03-09T22:55:37Z |
| format | Article |
| id | doaj.art-e267b7d537a844b8917698e8a02dc848 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T22:55:37Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e267b7d537a844b8917698e8a02dc8482023-11-23T18:12:21ZengMDPI AGMolecules1420-30492022-06-012712384810.3390/molecules27123848Indoxyl Sulfate Induces Oxidative Changes in Plasma and HemolysateAnna Pieniazek0Michal Kopera1Lukasz Gwozdzinski2Krzysztof Gwozdzinski3Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Pharmacology and Toxicology, Medical University of Lodz, 90-753 Lodz, PolandDepartment of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandThe deteriorating function of the kidneys in chronic kidney disease (CKD) is associated, among other things, with the retention of many unnecessary metabolic products in the body. Indoxyl sulfate (IS) belongs to the group of uremic toxins with a high protein binding affinity. Moreover, this compound can generate oxidative stress. We hypothesized that a high concentration of IS might induce oxidative changes in erythrocytes and plasma components, and could therefore contribute to CKD progression. In this study, we evaluated the influence of IS on the oxidative stress parameters in plasma and hemolysate. Moreover, as a result of the action of IS, we observed a decrease in the total antioxidant capacity and a change in the activity of catalase and superoxide dismutase in hemolysate and plasma. The obtained results indicate that IS induces oxidative damage to hemolysate and plasma components. Greater changes in the parameters of oxidative stress were observed in hemolysate than in plasma treated with indoxyl sulfate. The obtained results suggest that the increased concentration of IS in patients with chronic kidney disease may lead to a decrease in the lifespan of erythrocytes in their bloodstream.https://www.mdpi.com/1420-3049/27/12/3848indoxyl sulfatehemolysateplasmaoxidative stress |
| spellingShingle | Anna Pieniazek Michal Kopera Lukasz Gwozdzinski Krzysztof Gwozdzinski Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate Molecules indoxyl sulfate hemolysate plasma oxidative stress |
| title | Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate |
| title_full | Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate |
| title_fullStr | Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate |
| title_full_unstemmed | Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate |
| title_short | Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate |
| title_sort | indoxyl sulfate induces oxidative changes in plasma and hemolysate |
| topic | indoxyl sulfate hemolysate plasma oxidative stress |
| url | https://www.mdpi.com/1420-3049/27/12/3848 |
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