Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation
Polycaprolactone nanofibers are used as scaffolds in the field of tissue engineering for tissue regeneration or drug delivery. Polycaprolactone (PCL) is a biodegradable hydrophobic polyester used to obtain implantable nanostructures, which are clinically applicable due to their biological safety. Po...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/6/727 |
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| author | Stefania Lama Amalia Luce Giuseppe Bitti Pilar Chacon-Millan Annalisa Itro Pasquale Ferranti Giovanni D’Auria Marcella Cammarota Giovanni Francesco Nicoletti Giuseppe Andrea Ferraro Chiara Schiraldi Michele Caraglia Evzen Amler Paola Stiuso |
| author_facet | Stefania Lama Amalia Luce Giuseppe Bitti Pilar Chacon-Millan Annalisa Itro Pasquale Ferranti Giovanni D’Auria Marcella Cammarota Giovanni Francesco Nicoletti Giuseppe Andrea Ferraro Chiara Schiraldi Michele Caraglia Evzen Amler Paola Stiuso |
| author_sort | Stefania Lama |
| collection | DOAJ |
| description | Polycaprolactone nanofibers are used as scaffolds in the field of tissue engineering for tissue regeneration or drug delivery. Polycaprolactone (PCL) is a biodegradable hydrophobic polyester used to obtain implantable nanostructures, which are clinically applicable due to their biological safety. Polydatin (PD), a glycosidic precursor of resveratrol, is known for its antioxidant, antitumor, antiosteoporotic, and bone regeneration activities. We aimed to use the osteogenic capacity of polydatin to create a biomimetic innovative and patented scaffold consisting of PCL-PD for bone tissue engineering. Both osteosarcoma cells (Saos-2) and mesenchymal stem cells (MSCs) were used to test the in vitro cytocompatibility of the PD-PCL scaffold. Reverse-phase (RP) HPLC was used to evaluate the timing release of PD from the PCL-PD nanofibers and the MTT assay, scanning electron microscopy, and alkaline phosphatase (ALP) activity were used to evaluate the proliferation, adhesion, and cellular differentiation in both osteosarcoma and human mesenchymal stem cells (MSCs) seeded on PD-PCL nanofibers. The proliferation of osteosarcoma cells (Saos-2) on the PD-PCL scaffold decreased when compared to cells grown on PLC nanofibers, whereas the proliferation of MSCs was comparable in both PCL and PD-PCL nanofibers. Noteworthy, after 14 days, the ALP activity was higher in both Saos-2 cells and MSCs cultivated on PD-PCL than on empty scaffolds. Moreover, the same cells showed a spindle-shaped morphology after 14 days when grown on PD-PCL as shown by SEM. In conclusion, we provide evidence that nanofibers appropriately coated with PD support the adhesion and promote the osteogenic differentiation of both human osteosarcoma cells and MSCs. |
| first_indexed | 2024-03-09T22:46:55Z |
| format | Article |
| id | doaj.art-e26bc70f706d43bcbf878ef2a6eb111b |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T22:46:55Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-e26bc70f706d43bcbf878ef2a6eb111b2023-11-23T18:27:42ZengMDPI AGPharmaceuticals1424-82472022-06-0115672710.3390/ph15060727Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic DifferentiationStefania Lama0Amalia Luce1Giuseppe Bitti2Pilar Chacon-Millan3Annalisa Itro4Pasquale Ferranti5Giovanni D’Auria6Marcella Cammarota7Giovanni Francesco Nicoletti8Giuseppe Andrea Ferraro9Chiara Schiraldi10Michele Caraglia11Evzen Amler12Paola Stiuso13Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyInstitute of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 15006 Prague, Czech RepublicDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyPlastic Surgery Unit, Department of Multidisciplinary Medical and Dental Specialties, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, 80138 Portici, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, 80138 Portici, ItalyDepartment of Experimental Medicine, Section of Biotechnology, Molecular Medicine and Medical Histology, University of Campania “L. Vanvitelli”, 80138 Naples, ItalyPlastic Surgery Unit, Department of Multidisciplinary Medical and Dental Specialties, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyPlastic Surgery Unit, Department of Multidisciplinary Medical and Dental Specialties, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Biotechnology, Molecular Medicine and Medical Histology, University of Campania “L. Vanvitelli”, 80138 Naples, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyInstitute of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 15006 Prague, Czech RepublicDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyPolycaprolactone nanofibers are used as scaffolds in the field of tissue engineering for tissue regeneration or drug delivery. Polycaprolactone (PCL) is a biodegradable hydrophobic polyester used to obtain implantable nanostructures, which are clinically applicable due to their biological safety. Polydatin (PD), a glycosidic precursor of resveratrol, is known for its antioxidant, antitumor, antiosteoporotic, and bone regeneration activities. We aimed to use the osteogenic capacity of polydatin to create a biomimetic innovative and patented scaffold consisting of PCL-PD for bone tissue engineering. Both osteosarcoma cells (Saos-2) and mesenchymal stem cells (MSCs) were used to test the in vitro cytocompatibility of the PD-PCL scaffold. Reverse-phase (RP) HPLC was used to evaluate the timing release of PD from the PCL-PD nanofibers and the MTT assay, scanning electron microscopy, and alkaline phosphatase (ALP) activity were used to evaluate the proliferation, adhesion, and cellular differentiation in both osteosarcoma and human mesenchymal stem cells (MSCs) seeded on PD-PCL nanofibers. The proliferation of osteosarcoma cells (Saos-2) on the PD-PCL scaffold decreased when compared to cells grown on PLC nanofibers, whereas the proliferation of MSCs was comparable in both PCL and PD-PCL nanofibers. Noteworthy, after 14 days, the ALP activity was higher in both Saos-2 cells and MSCs cultivated on PD-PCL than on empty scaffolds. Moreover, the same cells showed a spindle-shaped morphology after 14 days when grown on PD-PCL as shown by SEM. In conclusion, we provide evidence that nanofibers appropriately coated with PD support the adhesion and promote the osteogenic differentiation of both human osteosarcoma cells and MSCs.https://www.mdpi.com/1424-8247/15/6/727osteosarcomapolydatinosteogenic differentiationmesenchymal stem cellspolycaprolactone nanofibers |
| spellingShingle | Stefania Lama Amalia Luce Giuseppe Bitti Pilar Chacon-Millan Annalisa Itro Pasquale Ferranti Giovanni D’Auria Marcella Cammarota Giovanni Francesco Nicoletti Giuseppe Andrea Ferraro Chiara Schiraldi Michele Caraglia Evzen Amler Paola Stiuso Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation Pharmaceuticals osteosarcoma polydatin osteogenic differentiation mesenchymal stem cells polycaprolactone nanofibers |
| title | Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation |
| title_full | Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation |
| title_fullStr | Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation |
| title_full_unstemmed | Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation |
| title_short | Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation |
| title_sort | polydatin incorporated in polycaprolactone nanofibers improves osteogenic differentiation |
| topic | osteosarcoma polydatin osteogenic differentiation mesenchymal stem cells polycaprolactone nanofibers |
| url | https://www.mdpi.com/1424-8247/15/6/727 |
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