Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis
Abstract In the present study, the complete characterization of cDNA and genomic sequences of IL-1β and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1β cDNA was composed of 1208 nucleotides that wou...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2020-11-01
|
| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600204&tlng=en |
| _version_ | 1798022016912064512 |
|---|---|
| author | Mateus Maldonado Carriero Flavio Henrique-Silva Caroline Munhoz Meira Igor Mateus Queiroz Gato Alexandre Rodrigues Caetano Francisco Pereira Lobo Anderson Luis Alves Eduardo Sousa Varela Antonio Augusto Mendes Maia |
| author_facet | Mateus Maldonado Carriero Flavio Henrique-Silva Caroline Munhoz Meira Igor Mateus Queiroz Gato Alexandre Rodrigues Caetano Francisco Pereira Lobo Anderson Luis Alves Eduardo Sousa Varela Antonio Augusto Mendes Maia |
| author_sort | Mateus Maldonado Carriero |
| collection | DOAJ |
| description | Abstract In the present study, the complete characterization of cDNA and genomic sequences of IL-1β and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1β cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1β, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1β and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups. |
| first_indexed | 2024-04-11T17:22:56Z |
| format | Article |
| id | doaj.art-e270801a8ba64a5d82af68861ffb95aa |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-04-11T17:22:56Z |
| publishDate | 2020-11-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-e270801a8ba64a5d82af68861ffb95aa2022-12-22T04:12:27ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852020-11-0143410.1590/1678-4685-gmb-2020-0006Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensisMateus Maldonado Carrierohttps://orcid.org/0000-0002-8949-4400Flavio Henrique-SilvaCaroline Munhoz MeiraIgor Mateus Queiroz GatoAlexandre Rodrigues Caetanohttps://orcid.org/0000-0002-3419-7337Francisco Pereira Lobohttps://orcid.org/0000-0002-9671-9313Anderson Luis AlvesEduardo Sousa VarelaAntonio Augusto Mendes MaiaAbstract In the present study, the complete characterization of cDNA and genomic sequences of IL-1β and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1β cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1β, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1β and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600204&tlng=enBacterial infectionfish immunologygene expressioninterleukinSouth American fish |
| spellingShingle | Mateus Maldonado Carriero Flavio Henrique-Silva Caroline Munhoz Meira Igor Mateus Queiroz Gato Alexandre Rodrigues Caetano Francisco Pereira Lobo Anderson Luis Alves Eduardo Sousa Varela Antonio Augusto Mendes Maia Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis Genetics and Molecular Biology Bacterial infection fish immunology gene expression interleukin South American fish |
| title | Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis |
| title_full | Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis |
| title_fullStr | Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis |
| title_full_unstemmed | Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis |
| title_short | Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis |
| title_sort | molecular characterization and gene expression analysis of the pro inflammatory cytokines il 1β and il 8 in the south american fish piaractus mesopotamicus challenged with aeromonas dhakensis |
| topic | Bacterial infection fish immunology gene expression interleukin South American fish |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000600204&tlng=en |
| work_keys_str_mv | AT mateusmaldonadocarriero molecularcharacterizationandgeneexpressionanalysisoftheproinflammatorycytokinesil1bandil8inthesouthamericanfishpiaractusmesopotamicuschallengedwithaeromonasdhakensis AT flaviohenriquesilva molecularcharacterizationandgeneexpressionanalysisoftheproinflammatorycytokinesil1bandil8inthesouthamericanfishpiaractusmesopotamicuschallengedwithaeromonasdhakensis AT carolinemunhozmeira molecularcharacterizationandgeneexpressionanalysisoftheproinflammatorycytokinesil1bandil8inthesouthamericanfishpiaractusmesopotamicuschallengedwithaeromonasdhakensis AT igormateusqueirozgato molecularcharacterizationandgeneexpressionanalysisoftheproinflammatorycytokinesil1bandil8inthesouthamericanfishpiaractusmesopotamicuschallengedwithaeromonasdhakensis AT alexandrerodriguescaetano molecularcharacterizationandgeneexpressionanalysisoftheproinflammatorycytokinesil1bandil8inthesouthamericanfishpiaractusmesopotamicuschallengedwithaeromonasdhakensis AT franciscopereiralobo molecularcharacterizationandgeneexpressionanalysisoftheproinflammatorycytokinesil1bandil8inthesouthamericanfishpiaractusmesopotamicuschallengedwithaeromonasdhakensis AT andersonluisalves molecularcharacterizationandgeneexpressionanalysisoftheproinflammatorycytokinesil1bandil8inthesouthamericanfishpiaractusmesopotamicuschallengedwithaeromonasdhakensis AT eduardosousavarela molecularcharacterizationandgeneexpressionanalysisoftheproinflammatorycytokinesil1bandil8inthesouthamericanfishpiaractusmesopotamicuschallengedwithaeromonasdhakensis AT antonioaugustomendesmaia molecularcharacterizationandgeneexpressionanalysisoftheproinflammatorycytokinesil1bandil8inthesouthamericanfishpiaractusmesopotamicuschallengedwithaeromonasdhakensis |