A Study of the Antioxidant and Anti-Inflammatory Effects of Dusokohwaeum
Background: The aim of this study is to determine the antioxidant and anti-inflammatory effects of Dusokohwaeum (DOE). Methods: To measure the antioxidant and anti-inflammatory effects of DOE, the total flavonoid and polyphenol contents and radical scavenging activity were measured. Furthermore,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
E-Tree Publishing
2023-11-01
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| Series: | Journal of Acupuncture Research |
| Subjects: |
| Summary: | Background: The aim of this study is to determine the antioxidant and anti-inflammatory
effects of Dusokohwaeum (DOE).
Methods: To measure the antioxidant and anti-inflammatory effects of DOE,
the total flavonoid and polyphenol contents and radical scavenging activity were
measured. Furthermore, reactive oxygen species (ROS), nitric oxide, and cytokine
production were measured by treating lipopolysaccharide-induced RAW264.7 cells
with DOE, and gene expression levels of inducible cyclooxygenase-2, nitric oxide
synthase, and cytokines were evaluated.
Results: Radical scavenging experiments revealed a significant concentration-dependent
increase in scavenging capacity. The production of ROS, nitric oxide,
and cytokines in the cells showed a significant concentration-dependent decrease
when compared with the control group. The gene expression levels of inducible
cyclooxygenase-2, nitric oxide synthase, and cytokines also showed a significant
concentration-dependent decrease when compared with the control group.
Conclusion: Interestingly, the antioxidant and anti-inflammatory effects of DOE
were 23.42 ± 0.64 mg GAE/g and 20.83 ± 0.98 mg QE/g, respectively. The administration
of DOE resulted in a concentration-dependent increase in scavenging
ability in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-
6-sulfonic acid) (ABTS) radical scavenging ability experiments. The
production of intracellular ROS and nitric oxide was significantly reduced in the
presence of DOE. The production of inflammatory cytokines (prostaglandin E2,
tumor necrosis factor-alpha [TNF-α], interleukin-1 beta [IL-1β], and IL-6) was
significantly reduced in the presence of DOE. Finally, the expression levels of inducible
nitric oxide synthase, cyclooxygenase-2, TNF-α, IL-1β, and IL-6 were significantly
decreased in the presence of DOE. |
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| ISSN: | 2586-288X 2586-2898 |