A preliminary study of the immunogenic response of plant-derived multi-epitopic peptide vaccine candidate of Mycoplasma gallisepticum in chickens
Mycoplasma gallisepticum (MG) is responsible for chronic respiratory disease in avian species, characterized by symptoms like respiratory rales and coughing. Existing vaccines for MG have limited efficacy and require multiple doses. Certain MG cytoadherence proteins (GapA, CrmA, PlpA, and Hlp3) play...
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1298880/full |
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| author | Susithra Priyadarhni Mugunthan Divyadharshini Venkatesan Chandramohan Govindasamy Dhivya Selvaraj Harish Mani Chandra |
| author_facet | Susithra Priyadarhni Mugunthan Divyadharshini Venkatesan Chandramohan Govindasamy Dhivya Selvaraj Harish Mani Chandra |
| author_sort | Susithra Priyadarhni Mugunthan |
| collection | DOAJ |
| description | Mycoplasma gallisepticum (MG) is responsible for chronic respiratory disease in avian species, characterized by symptoms like respiratory rales and coughing. Existing vaccines for MG have limited efficacy and require multiple doses. Certain MG cytoadherence proteins (GapA, CrmA, PlpA, and Hlp3) play a crucial role in the pathogen’s respiratory tract colonization and infection. Plant-based proteins and therapeutics have gained attention due to their safety and efficiency. In this study, we designed a 21.4-kDa multi-epitope peptide vaccine (MEPV) using immunogenic segments from cytoadherence proteins. The MEPV’s effectiveness was verified through computational simulations. We then cloned the MEPV, introduced it into the plant expression vector pSiM24-eGFP, and expressed it in Nicotiana benthamiana leaves. The plant-produced MEPV proved to be immunogenic when administered intramuscularly to chickens. It significantly boosted the production of immunoglobulin Y (IgY)-neutralizing antibodies against cytoadherence protein epitopes in immunized chickens compared to that in the control group. This preliminary investigation demonstrates that the plant-derived MEPV is effective in triggering an immune response in chickens. To establish an efficient poultry health management system and ensure the sustainability of the poultry industry, further research is needed to develop avian vaccines using plant biotechnology. |
| first_indexed | 2024-03-08T12:03:07Z |
| format | Article |
| id | doaj.art-e276053a3880446d933ec3668bfb7abc |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T12:03:07Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e276053a3880446d933ec3668bfb7abc2024-01-23T18:17:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-01-011410.3389/fpls.2023.12988801298880A preliminary study of the immunogenic response of plant-derived multi-epitopic peptide vaccine candidate of Mycoplasma gallisepticum in chickensSusithra Priyadarhni Mugunthan0Divyadharshini Venkatesan1Chandramohan Govindasamy2Dhivya Selvaraj3Harish Mani Chandra4Department of Biotechnology, Thiruvalluvar University, Vellore, Tamil Nadu, IndiaDepartment of Biotechnology, Thiruvalluvar University, Vellore, Tamil Nadu, IndiaDepartment of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi ArabiaArtificial Intelligence Laboratory, School of Computer Information and Communication Engineering, Kunsan National University, Gunsan, Republic of KoreaDepartment of Biotechnology, Thiruvalluvar University, Vellore, Tamil Nadu, IndiaMycoplasma gallisepticum (MG) is responsible for chronic respiratory disease in avian species, characterized by symptoms like respiratory rales and coughing. Existing vaccines for MG have limited efficacy and require multiple doses. Certain MG cytoadherence proteins (GapA, CrmA, PlpA, and Hlp3) play a crucial role in the pathogen’s respiratory tract colonization and infection. Plant-based proteins and therapeutics have gained attention due to their safety and efficiency. In this study, we designed a 21.4-kDa multi-epitope peptide vaccine (MEPV) using immunogenic segments from cytoadherence proteins. The MEPV’s effectiveness was verified through computational simulations. We then cloned the MEPV, introduced it into the plant expression vector pSiM24-eGFP, and expressed it in Nicotiana benthamiana leaves. The plant-produced MEPV proved to be immunogenic when administered intramuscularly to chickens. It significantly boosted the production of immunoglobulin Y (IgY)-neutralizing antibodies against cytoadherence protein epitopes in immunized chickens compared to that in the control group. This preliminary investigation demonstrates that the plant-derived MEPV is effective in triggering an immune response in chickens. To establish an efficient poultry health management system and ensure the sustainability of the poultry industry, further research is needed to develop avian vaccines using plant biotechnology.https://www.frontiersin.org/articles/10.3389/fpls.2023.1298880/fullplant made vaccinesMycoplasma gallisepticummulti-epitopeAvian mycoplasmaplant expression system |
| spellingShingle | Susithra Priyadarhni Mugunthan Divyadharshini Venkatesan Chandramohan Govindasamy Dhivya Selvaraj Harish Mani Chandra A preliminary study of the immunogenic response of plant-derived multi-epitopic peptide vaccine candidate of Mycoplasma gallisepticum in chickens Frontiers in Plant Science plant made vaccines Mycoplasma gallisepticum multi-epitope Avian mycoplasma plant expression system |
| title | A preliminary study of the immunogenic response of plant-derived multi-epitopic peptide vaccine candidate of Mycoplasma gallisepticum in chickens |
| title_full | A preliminary study of the immunogenic response of plant-derived multi-epitopic peptide vaccine candidate of Mycoplasma gallisepticum in chickens |
| title_fullStr | A preliminary study of the immunogenic response of plant-derived multi-epitopic peptide vaccine candidate of Mycoplasma gallisepticum in chickens |
| title_full_unstemmed | A preliminary study of the immunogenic response of plant-derived multi-epitopic peptide vaccine candidate of Mycoplasma gallisepticum in chickens |
| title_short | A preliminary study of the immunogenic response of plant-derived multi-epitopic peptide vaccine candidate of Mycoplasma gallisepticum in chickens |
| title_sort | preliminary study of the immunogenic response of plant derived multi epitopic peptide vaccine candidate of mycoplasma gallisepticum in chickens |
| topic | plant made vaccines Mycoplasma gallisepticum multi-epitope Avian mycoplasma plant expression system |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1298880/full |
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