Pigment Epithelium-Derived Factor Promotes the Growth and Migration of Human Esophageal Squamous Cell Carcinoma
Pigment epithelium-derived factor (PEDF) is an oncogene found in various types of cancers. However, how PEDF affects the development of human esophageal squamous cell carcinoma (ESCC) is unknown. This study investigates the role of PEDF in ESCC cell proliferation, migration, and cell cycle both in v...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2019.01520/full |
| _version_ | 1818310908777070592 |
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| author | De-Rong Tang Cheng-Lin Li Ke-Ping Xu Qing-Quan Wu Qi-You Chen Jun-Jie Lv Jian Ji Bao Zang Chen Chen Biao Gu Jian-Qiang Zhao |
| author_facet | De-Rong Tang Cheng-Lin Li Ke-Ping Xu Qing-Quan Wu Qi-You Chen Jun-Jie Lv Jian Ji Bao Zang Chen Chen Biao Gu Jian-Qiang Zhao |
| author_sort | De-Rong Tang |
| collection | DOAJ |
| description | Pigment epithelium-derived factor (PEDF) is an oncogene found in various types of cancers. However, how PEDF affects the development of human esophageal squamous cell carcinoma (ESCC) is unknown. This study investigates the role of PEDF in ESCC cell proliferation, migration, and cell cycle both in vitro and in vivo. The PEDF expression was examined in patient tumor samples and ESCC cell lines. Short hairpin RNA technology was used to inhibit the PEDF expression in ESCC EC9706 and KYSE150 cells. In vitro cell proliferation and migration assays were performed. The effects of PEDF on tumor growth and progression were examined in vivo in murine subcutaneous xenograft tumor models. It was found that PEDF was overexpressed in esophageal cancer cells and patient tumor tissues compared to normal control samples. PEDF enhanced cell cycle progression and inhibited cell apoptosis. Knock down of PEDF inhibited esophageal cell proliferation and migration in vitro. Moreover, Inhibition of PEDF significantly reduced tumor growth and tumor size in vivo. These results indicate that PEDF induce tumorigenesis in ESCC and can be a potential therapeutic target for cancer treatment. |
| first_indexed | 2024-12-13T07:53:32Z |
| format | Article |
| id | doaj.art-e2783d2566044d158dbfe94a428f086c |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-13T07:53:32Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-e2783d2566044d158dbfe94a428f086c2022-12-21T23:54:35ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-01-01910.3389/fonc.2019.01520492490Pigment Epithelium-Derived Factor Promotes the Growth and Migration of Human Esophageal Squamous Cell CarcinomaDe-Rong TangCheng-Lin LiKe-Ping XuQing-Quan WuQi-You ChenJun-Jie LvJian JiBao ZangChen ChenBiao GuJian-Qiang ZhaoPigment epithelium-derived factor (PEDF) is an oncogene found in various types of cancers. However, how PEDF affects the development of human esophageal squamous cell carcinoma (ESCC) is unknown. This study investigates the role of PEDF in ESCC cell proliferation, migration, and cell cycle both in vitro and in vivo. The PEDF expression was examined in patient tumor samples and ESCC cell lines. Short hairpin RNA technology was used to inhibit the PEDF expression in ESCC EC9706 and KYSE150 cells. In vitro cell proliferation and migration assays were performed. The effects of PEDF on tumor growth and progression were examined in vivo in murine subcutaneous xenograft tumor models. It was found that PEDF was overexpressed in esophageal cancer cells and patient tumor tissues compared to normal control samples. PEDF enhanced cell cycle progression and inhibited cell apoptosis. Knock down of PEDF inhibited esophageal cell proliferation and migration in vitro. Moreover, Inhibition of PEDF significantly reduced tumor growth and tumor size in vivo. These results indicate that PEDF induce tumorigenesis in ESCC and can be a potential therapeutic target for cancer treatment.https://www.frontiersin.org/article/10.3389/fonc.2019.01520/fullpigment epithelium-derived factor (PEDF)esophageal carcinomatumorigenesisproliferationmigration |
| spellingShingle | De-Rong Tang Cheng-Lin Li Ke-Ping Xu Qing-Quan Wu Qi-You Chen Jun-Jie Lv Jian Ji Bao Zang Chen Chen Biao Gu Jian-Qiang Zhao Pigment Epithelium-Derived Factor Promotes the Growth and Migration of Human Esophageal Squamous Cell Carcinoma Frontiers in Oncology pigment epithelium-derived factor (PEDF) esophageal carcinoma tumorigenesis proliferation migration |
| title | Pigment Epithelium-Derived Factor Promotes the Growth and Migration of Human Esophageal Squamous Cell Carcinoma |
| title_full | Pigment Epithelium-Derived Factor Promotes the Growth and Migration of Human Esophageal Squamous Cell Carcinoma |
| title_fullStr | Pigment Epithelium-Derived Factor Promotes the Growth and Migration of Human Esophageal Squamous Cell Carcinoma |
| title_full_unstemmed | Pigment Epithelium-Derived Factor Promotes the Growth and Migration of Human Esophageal Squamous Cell Carcinoma |
| title_short | Pigment Epithelium-Derived Factor Promotes the Growth and Migration of Human Esophageal Squamous Cell Carcinoma |
| title_sort | pigment epithelium derived factor promotes the growth and migration of human esophageal squamous cell carcinoma |
| topic | pigment epithelium-derived factor (PEDF) esophageal carcinoma tumorigenesis proliferation migration |
| url | https://www.frontiersin.org/article/10.3389/fonc.2019.01520/full |
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