Tissue culture-based Agrobacterium-mediated and in planta transformation methods
Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requ...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2017-12-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201704-0002_tissue-culture-based-agrobacterium-mediated-and-in-planta-transformation-methods.php |
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| author | Mohsen NIAZIAN Seyed Ahmad SADAT NOORI Petr GALUSZKA Seyed Mohammad Mahdi MORTAZAVIAN |
| author_facet | Mohsen NIAZIAN Seyed Ahmad SADAT NOORI Petr GALUSZKA Seyed Mohammad Mahdi MORTAZAVIAN |
| author_sort | Mohsen NIAZIAN |
| collection | DOAJ |
| description | Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. Development of genotype-independent gene transformation method is of great interest in many plants. Some tissue culture-independent Agrobacterium-mediated gene transformation methods are reported in individual plants and crops. Generally, these methods are called in planta gene transformation. In planta transformation methods are free from somaclonal variation and easier, quicker, and simpler than tissue culture-based transformation methods. Vacuum infiltration, injection of Agrobacterium culture to plant tissues, pollen-tube pathway, floral dip and floral spray are the main methods of in planta transformation. Each of these methods has its own advantages and disadvantages. Simplicity and reliability are the primary reasons for the popularity of the in planta methods. These methods are much quicker than regular tissue culture-based Agrobacterium-mediated gene transformation and success can be achieved by non-experts. In the present review, we highlight all methods of in planta transformation comparing them with regular tissue culture-based Agrobacterium-mediated transformation methods and then recently successful transformations using these methods are presented. |
| first_indexed | 2024-04-10T08:29:56Z |
| format | Article |
| id | doaj.art-e27d048f965e40599ebb2eb8c164eed2 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:56Z |
| publishDate | 2017-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-e27d048f965e40599ebb2eb8c164eed22023-02-23T03:30:38ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252017-12-0153413314310.17221/177/2016-CJGPBcjg-201704-0002Tissue culture-based Agrobacterium-mediated and in planta transformation methodsMohsen NIAZIAN0Seyed Ahmad SADAT NOORI1Petr GALUSZKA2Seyed Mohammad Mahdi MORTAZAVIAN3Department of Agronomy and Plant Breeding Science, College of Aburaihan, University of Tehran, Pakdasht, IranDepartment of Agronomy and Plant Breeding Science, College of Aburaihan, University of Tehran, Pakdasht, IranDepartment of Molecular Biology, Centre of the Haná Region for Biotechnological and Agriculture Research, Palacky University, Olomouc, Czech RepublicDepartment of Agronomy and Plant Breeding Science, College of Aburaihan, University of Tehran, Pakdasht, IranGene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. Development of genotype-independent gene transformation method is of great interest in many plants. Some tissue culture-independent Agrobacterium-mediated gene transformation methods are reported in individual plants and crops. Generally, these methods are called in planta gene transformation. In planta transformation methods are free from somaclonal variation and easier, quicker, and simpler than tissue culture-based transformation methods. Vacuum infiltration, injection of Agrobacterium culture to plant tissues, pollen-tube pathway, floral dip and floral spray are the main methods of in planta transformation. Each of these methods has its own advantages and disadvantages. Simplicity and reliability are the primary reasons for the popularity of the in planta methods. These methods are much quicker than regular tissue culture-based Agrobacterium-mediated gene transformation and success can be achieved by non-experts. In the present review, we highlight all methods of in planta transformation comparing them with regular tissue culture-based Agrobacterium-mediated transformation methods and then recently successful transformations using these methods are presented.https://cjgpb.agriculturejournals.cz/artkey/cjg-201704-0002_tissue-culture-based-agrobacterium-mediated-and-in-planta-transformation-methods.phpagrobacteriumfloral dipfloral spraypollen-tube pathwaysonicationvacuum infiltration |
| spellingShingle | Mohsen NIAZIAN Seyed Ahmad SADAT NOORI Petr GALUSZKA Seyed Mohammad Mahdi MORTAZAVIAN Tissue culture-based Agrobacterium-mediated and in planta transformation methods Czech Journal of Genetics and Plant Breeding agrobacterium floral dip floral spray pollen-tube pathway sonication vacuum infiltration |
| title | Tissue culture-based Agrobacterium-mediated and in planta transformation methods |
| title_full | Tissue culture-based Agrobacterium-mediated and in planta transformation methods |
| title_fullStr | Tissue culture-based Agrobacterium-mediated and in planta transformation methods |
| title_full_unstemmed | Tissue culture-based Agrobacterium-mediated and in planta transformation methods |
| title_short | Tissue culture-based Agrobacterium-mediated and in planta transformation methods |
| title_sort | tissue culture based agrobacterium mediated and in planta transformation methods |
| topic | agrobacterium floral dip floral spray pollen-tube pathway sonication vacuum infiltration |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201704-0002_tissue-culture-based-agrobacterium-mediated-and-in-planta-transformation-methods.php |
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