The NLRP3 Inflammasome Is Dispensable in Methicillin-Resistant <i>Staphylococcus aureus</i> Urinary Tract Infection
The NLRP3 inflammasome is a cytoplasmic complex that senses molecular patterns from pathogens or damaged cells to trigger an innate immune defense response marked by the production of proinflammatory cytokines IL-1β and IL-18 and an inflammatory death called pyroptosis. The NLRP3 inflammasome is act...
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MDPI AG
2024-01-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/13/2/106 |
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| author | Santosh Paudel Rahul Kumar Kenneth A. Rogers Yogesh Saini Sonika Patial Ritwij Kulkarni |
| author_facet | Santosh Paudel Rahul Kumar Kenneth A. Rogers Yogesh Saini Sonika Patial Ritwij Kulkarni |
| author_sort | Santosh Paudel |
| collection | DOAJ |
| description | The NLRP3 inflammasome is a cytoplasmic complex that senses molecular patterns from pathogens or damaged cells to trigger an innate immune defense response marked by the production of proinflammatory cytokines IL-1β and IL-18 and an inflammatory death called pyroptosis. The NLRP3 inflammasome is activated in the urinary tract by a variety of infectious and non-infectious insults. In this study, we investigated the role of the NLRP3 inflammasome by comparing the pathophysiology of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) ascending UTI in wild-type (WT) and <i>Nlrp3</i><sup>−/−</sup> mice. The difference in the bacterial burden detected in the urinary tracts of MRSA-infected WT and <i>Nlrp3</i><sup>−/−</sup> was not statistically significant at 6, 24, and 72 h post-infection (hpi). The levels of pro-inflammatory cytokines and chemokines as well as the numbers of granulocytes recruited to bladder and kidney tissues at 24 hpi were also similar between <i>Nlrp3</i><sup>−/−</sup> and WT mice. The histopathological analysis of MRSA-infected bladder and kidney sections from <i>Nlrp3<sup>−/−</sup></i> and WT mice showed similar inflammation. Overall, these results suggest that MRSA-induced urinary NLRP3 activity does not play a role in the pathophysiology of the ascending UTI. |
| first_indexed | 2024-03-07T22:18:53Z |
| format | Article |
| id | doaj.art-e27d5680b07944b8a911e644e290e0bc |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-07T22:18:53Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-e27d5680b07944b8a911e644e290e0bc2024-02-23T15:30:13ZengMDPI AGPathogens2076-08172024-01-0113210610.3390/pathogens13020106The NLRP3 Inflammasome Is Dispensable in Methicillin-Resistant <i>Staphylococcus aureus</i> Urinary Tract InfectionSantosh Paudel0Rahul Kumar1Kenneth A. Rogers2Yogesh Saini3Sonika Patial4Ritwij Kulkarni5Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504, USADepartment of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USANew Iberia Research Center, University of Louisiana at Lafayette, Lafayette, LA 70560, USADepartment of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USANational Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, Durham, NC 27709, USADepartment of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504, USAThe NLRP3 inflammasome is a cytoplasmic complex that senses molecular patterns from pathogens or damaged cells to trigger an innate immune defense response marked by the production of proinflammatory cytokines IL-1β and IL-18 and an inflammatory death called pyroptosis. The NLRP3 inflammasome is activated in the urinary tract by a variety of infectious and non-infectious insults. In this study, we investigated the role of the NLRP3 inflammasome by comparing the pathophysiology of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) ascending UTI in wild-type (WT) and <i>Nlrp3</i><sup>−/−</sup> mice. The difference in the bacterial burden detected in the urinary tracts of MRSA-infected WT and <i>Nlrp3</i><sup>−/−</sup> was not statistically significant at 6, 24, and 72 h post-infection (hpi). The levels of pro-inflammatory cytokines and chemokines as well as the numbers of granulocytes recruited to bladder and kidney tissues at 24 hpi were also similar between <i>Nlrp3</i><sup>−/−</sup> and WT mice. The histopathological analysis of MRSA-infected bladder and kidney sections from <i>Nlrp3<sup>−/−</sup></i> and WT mice showed similar inflammation. Overall, these results suggest that MRSA-induced urinary NLRP3 activity does not play a role in the pathophysiology of the ascending UTI.https://www.mdpi.com/2076-0817/13/2/106MRSAUTINLRP3inflammasome |
| spellingShingle | Santosh Paudel Rahul Kumar Kenneth A. Rogers Yogesh Saini Sonika Patial Ritwij Kulkarni The NLRP3 Inflammasome Is Dispensable in Methicillin-Resistant <i>Staphylococcus aureus</i> Urinary Tract Infection Pathogens MRSA UTI NLRP3 inflammasome |
| title | The NLRP3 Inflammasome Is Dispensable in Methicillin-Resistant <i>Staphylococcus aureus</i> Urinary Tract Infection |
| title_full | The NLRP3 Inflammasome Is Dispensable in Methicillin-Resistant <i>Staphylococcus aureus</i> Urinary Tract Infection |
| title_fullStr | The NLRP3 Inflammasome Is Dispensable in Methicillin-Resistant <i>Staphylococcus aureus</i> Urinary Tract Infection |
| title_full_unstemmed | The NLRP3 Inflammasome Is Dispensable in Methicillin-Resistant <i>Staphylococcus aureus</i> Urinary Tract Infection |
| title_short | The NLRP3 Inflammasome Is Dispensable in Methicillin-Resistant <i>Staphylococcus aureus</i> Urinary Tract Infection |
| title_sort | nlrp3 inflammasome is dispensable in methicillin resistant i staphylococcus aureus i urinary tract infection |
| topic | MRSA UTI NLRP3 inflammasome |
| url | https://www.mdpi.com/2076-0817/13/2/106 |
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