The BCG Moreau Vaccine Upregulates In Vitro the Expression of TLR4, B7-1, Dectin-1 and EP2 on Human Monocytes
Background: Tuberculosis (TB) is currently the second greatest killer worldwide and is caused by a single infectious agent. Since Bacillus Calmette–Guérin (BCG) is the only vaccine currently in use against TB, studies addressing the protective role of BCG in the context of inducible surface biomarke...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/1/86 |
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| author | Paulo R. Z. Antas Andreon S. M. da Silva Lawrence H. P. Albuquerque Matheus R. Almeida Evelyn N. G. S. Pereira Luiz R. R. Castello-Branco Carlos G. G. de Ponte |
| author_facet | Paulo R. Z. Antas Andreon S. M. da Silva Lawrence H. P. Albuquerque Matheus R. Almeida Evelyn N. G. S. Pereira Luiz R. R. Castello-Branco Carlos G. G. de Ponte |
| author_sort | Paulo R. Z. Antas |
| collection | DOAJ |
| description | Background: Tuberculosis (TB) is currently the second greatest killer worldwide and is caused by a single infectious agent. Since Bacillus Calmette–Guérin (BCG) is the only vaccine currently in use against TB, studies addressing the protective role of BCG in the context of inducible surface biomarkers are urgently required for TB control. Methods: In this study, groups of HIV-negative adult healthy donors (HD; <i>n</i> = 22) and neonate samples (UCB; <i>n</i> = 48) were voluntarily enrolled. The BCG Moreau strain was used for the in vitro mononuclear cell infections. Subsequently, phenotyping tools were used for surface biomarker detection. Monocytes were assayed for TLR4, B7-1, Dectin-1, EP2, and TIM-3 expression levels. Results: At 48 h, the BCG Moreau induced the highest TLR4, B7-1, and Dectin-1 levels in the HD group only (<i>p</i>-value < 0.05). TIM-3 expression failed to be modulated after BCG infection. At 72 h, BCG Moreau equally induced the highest EP2 levels in the HD group (<i>p</i>-value < 0.005), and higher levels were also found in HD when compared with the UCB group (<i>p</i>-value < 0.05). Conclusions: This study uncovers critical roles for biomarkers after the instruction of host monocyte activation patterns. Understanding the regulation of human innate immune responses is critical for vaccine development and for treating infectious diseases. |
| first_indexed | 2024-03-09T11:04:37Z |
| format | Article |
| id | doaj.art-e27e8156bfd241f4a684e55f56d7b404 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T11:04:37Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-e27e8156bfd241f4a684e55f56d7b4042023-12-01T01:01:55ZengMDPI AGVaccines2076-393X2022-12-011118610.3390/vaccines11010086The BCG Moreau Vaccine Upregulates In Vitro the Expression of TLR4, B7-1, Dectin-1 and EP2 on Human MonocytesPaulo R. Z. Antas0Andreon S. M. da Silva1Lawrence H. P. Albuquerque2Matheus R. Almeida3Evelyn N. G. S. Pereira4Luiz R. R. Castello-Branco5Carlos G. G. de Ponte6Laboratório de Imunologia Clínica, Instituto Oswaldo Cruz, Fiocruz, and Instituto Nacional de Ciência e Tecnologia em Tuberculose (INCT-TB), Rio de Janeiro 21040-900, BrazilLaboratório de Imunologia Clínica, Instituto Oswaldo Cruz, Fiocruz, and Instituto Nacional de Ciência e Tecnologia em Tuberculose (INCT-TB), Rio de Janeiro 21040-900, BrazilLaboratório de Imunologia Clínica, Instituto Oswaldo Cruz, Fiocruz, and Instituto Nacional de Ciência e Tecnologia em Tuberculose (INCT-TB), Rio de Janeiro 21040-900, BrazilLaboratório de Imunologia Clínica, Instituto Oswaldo Cruz, Fiocruz, and Instituto Nacional de Ciência e Tecnologia em Tuberculose (INCT-TB), Rio de Janeiro 21040-900, BrazilLaboratório de Investigação Cardiovascular, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratório de Imunologia Clínica, Instituto Oswaldo Cruz, Fiocruz, and Instituto Nacional de Ciência e Tecnologia em Tuberculose (INCT-TB), Rio de Janeiro 21040-900, BrazilLaboratório de Imunologia Clínica, Instituto Oswaldo Cruz, Fiocruz, and Instituto Nacional de Ciência e Tecnologia em Tuberculose (INCT-TB), Rio de Janeiro 21040-900, BrazilBackground: Tuberculosis (TB) is currently the second greatest killer worldwide and is caused by a single infectious agent. Since Bacillus Calmette–Guérin (BCG) is the only vaccine currently in use against TB, studies addressing the protective role of BCG in the context of inducible surface biomarkers are urgently required for TB control. Methods: In this study, groups of HIV-negative adult healthy donors (HD; <i>n</i> = 22) and neonate samples (UCB; <i>n</i> = 48) were voluntarily enrolled. The BCG Moreau strain was used for the in vitro mononuclear cell infections. Subsequently, phenotyping tools were used for surface biomarker detection. Monocytes were assayed for TLR4, B7-1, Dectin-1, EP2, and TIM-3 expression levels. Results: At 48 h, the BCG Moreau induced the highest TLR4, B7-1, and Dectin-1 levels in the HD group only (<i>p</i>-value < 0.05). TIM-3 expression failed to be modulated after BCG infection. At 72 h, BCG Moreau equally induced the highest EP2 levels in the HD group (<i>p</i>-value < 0.005), and higher levels were also found in HD when compared with the UCB group (<i>p</i>-value < 0.05). Conclusions: This study uncovers critical roles for biomarkers after the instruction of host monocyte activation patterns. Understanding the regulation of human innate immune responses is critical for vaccine development and for treating infectious diseases.https://www.mdpi.com/2076-393X/11/1/86BCG vaccinetuberculosismonocyteinnate immunitybiomarker |
| spellingShingle | Paulo R. Z. Antas Andreon S. M. da Silva Lawrence H. P. Albuquerque Matheus R. Almeida Evelyn N. G. S. Pereira Luiz R. R. Castello-Branco Carlos G. G. de Ponte The BCG Moreau Vaccine Upregulates In Vitro the Expression of TLR4, B7-1, Dectin-1 and EP2 on Human Monocytes Vaccines BCG vaccine tuberculosis monocyte innate immunity biomarker |
| title | The BCG Moreau Vaccine Upregulates In Vitro the Expression of TLR4, B7-1, Dectin-1 and EP2 on Human Monocytes |
| title_full | The BCG Moreau Vaccine Upregulates In Vitro the Expression of TLR4, B7-1, Dectin-1 and EP2 on Human Monocytes |
| title_fullStr | The BCG Moreau Vaccine Upregulates In Vitro the Expression of TLR4, B7-1, Dectin-1 and EP2 on Human Monocytes |
| title_full_unstemmed | The BCG Moreau Vaccine Upregulates In Vitro the Expression of TLR4, B7-1, Dectin-1 and EP2 on Human Monocytes |
| title_short | The BCG Moreau Vaccine Upregulates In Vitro the Expression of TLR4, B7-1, Dectin-1 and EP2 on Human Monocytes |
| title_sort | bcg moreau vaccine upregulates in vitro the expression of tlr4 b7 1 dectin 1 and ep2 on human monocytes |
| topic | BCG vaccine tuberculosis monocyte innate immunity biomarker |
| url | https://www.mdpi.com/2076-393X/11/1/86 |
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