A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope
The volumetric imaging of two-photon microscopy expands the focal depth and improves the throughput, which has unparalleled superiority for three-dimension samples, especially in neuroscience. However, emerging in volumetric imaging is still largely customized, which limits the integration with comm...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2023-07-01
|
| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545823500177 |
| _version_ | 1797777307214020608 |
|---|---|
| author | Ting Mo Yiran Liu Fatao Bie Zimin Dai Jin Chang Hui Gong Wei Zhou |
| author_facet | Ting Mo Yiran Liu Fatao Bie Zimin Dai Jin Chang Hui Gong Wei Zhou |
| author_sort | Ting Mo |
| collection | DOAJ |
| description | The volumetric imaging of two-photon microscopy expands the focal depth and improves the throughput, which has unparalleled superiority for three-dimension samples, especially in neuroscience. However, emerging in volumetric imaging is still largely customized, which limits the integration with commercial two-photon systems. Here, we analyzed the key parameters that modulate the focal depth and lateral resolution of polarized annular imaging and proposed a volumetric imaging module that can be directly integrated into commercial two-photon systems using conventional optical elements. This design incorporates the beam diameter adjustment settings of commercial two-photon systems, allowing flexibility to adjust the depth of focus while maintaining the same lateral resolution. Further, the depth range and lateral resolution of the design were verified, and the imaging throughput was demonstrated by an increase in the number of imaging neurons in the awake mouse cerebral cortex. |
| first_indexed | 2024-03-12T23:02:13Z |
| format | Article |
| id | doaj.art-e27ee1a6cae64a4f8e1ae9aadb1d4b9e |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-03-12T23:02:13Z |
| publishDate | 2023-07-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-e27ee1a6cae64a4f8e1ae9aadb1d4b9e2023-07-19T08:37:30ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052023-07-01160410.1142/S1793545823500177A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscopeTing Mo0Yiran Liu1Fatao Bie2Zimin Dai3Jin Chang4Hui Gong5Wei Zhou6Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, P. R. ChinaMoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, P. R. ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, P. R. ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, P. R. ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, P. R. ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, P. R. ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, P. R. ChinaThe volumetric imaging of two-photon microscopy expands the focal depth and improves the throughput, which has unparalleled superiority for three-dimension samples, especially in neuroscience. However, emerging in volumetric imaging is still largely customized, which limits the integration with commercial two-photon systems. Here, we analyzed the key parameters that modulate the focal depth and lateral resolution of polarized annular imaging and proposed a volumetric imaging module that can be directly integrated into commercial two-photon systems using conventional optical elements. This design incorporates the beam diameter adjustment settings of commercial two-photon systems, allowing flexibility to adjust the depth of focus while maintaining the same lateral resolution. Further, the depth range and lateral resolution of the design were verified, and the imaging throughput was demonstrated by an increase in the number of imaging neurons in the awake mouse cerebral cortex.https://www.worldscientific.com/doi/10.1142/S1793545823500177Volumetric imagingBessel beamtwo-photon microscopy |
| spellingShingle | Ting Mo Yiran Liu Fatao Bie Zimin Dai Jin Chang Hui Gong Wei Zhou A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope Journal of Innovative Optical Health Sciences Volumetric imaging Bessel beam two-photon microscopy |
| title | A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope |
| title_full | A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope |
| title_fullStr | A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope |
| title_full_unstemmed | A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope |
| title_short | A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope |
| title_sort | simple bessel module with tunable focal depth and constant resolution for commercial two photon microscope |
| topic | Volumetric imaging Bessel beam two-photon microscopy |
| url | https://www.worldscientific.com/doi/10.1142/S1793545823500177 |
| work_keys_str_mv | AT tingmo asimplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT yiranliu asimplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT fataobie asimplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT zimindai asimplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT jinchang asimplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT huigong asimplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT weizhou asimplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT tingmo simplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT yiranliu simplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT fataobie simplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT zimindai simplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT jinchang simplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT huigong simplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope AT weizhou simplebesselmodulewithtunablefocaldepthandconstantresolutionforcommercialtwophotonmicroscope |