Phytochemical constituents and antimicrobial evaluation of ethanolic extracts from Pleurotus spp. cultivated on substrate fortified with selenium
Edible mushrooms have the ability to bio-accumulate minerals from the growth medium into the fruiting body. Selenium (Se) is an example of minerals that can be easily absorbed by mushroom. This study investigated the phytochemical constituents and antimicrobial properties of <em>Pleurotus <...
| Format: | Article |
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| Language: | English |
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Arab Society for Fungal Conservation
2018-12-01
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| Series: | Microbial Biosystems Journal |
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| Online Access: | https://mb.journals.ekb.eg/article_26274_368eafea18edd45fbf2a2f4f32c133d9.pdf |
| _version_ | 1818209975125671936 |
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| collection | DOAJ |
| description | Edible mushrooms have the ability to bio-accumulate minerals from the growth medium into the fruiting body. Selenium (Se) is an example of minerals that can be easily absorbed by mushroom. This study investigated the phytochemical constituents and antimicrobial properties of <em>Pleurotus </em>spp. fortified with selenium. <em>Pleurotus</em> spp. (fortified with selenium and non-fortified with selenium) were cultivated on corn cobs using standard procedures. The yield, biological efficiency, selenium absorption level, phytochemical constituents and antimicrobial properties were determined using standard methods. Non-fortified <em>Pleurotus pulmonarius</em> recorded the highest yield and biological efficiency, while fortified <em>Pleurotus ostreatus </em>had the lowest yields and biological efficiency. There was no significant difference (P<0.05) in the selenium concentration absorbed in <em>Pleurotus ostreatus</em> (51.79µg/g) and <em>Pleurotus pulmonarius</em> (59.29 µg/g) but no selenium was detected in the non-fortified samples. The presence of 5 phytochemicals viz; saponins, alkaloids, phenols, tannins and flavonoids were detected in cultivated mushrooms. Saponin was highest (7.08 mg/g) in non-fortified <em>Pleurotus pulmonarius</em> while tannin was the least with the value of 0.02 mg/g in fortified <em>Pleurotus ostreatus</em>. The ethanolic extracts of <em>Pleurotus</em> spp. inhibited the growth of Gram positive and negative bacteria with <em>Pseudomonas aeruginosa</em>been the most susceptible (25.00 mm). The ethanolic extracts of fortified <em>Pleurotus </em>spp. inhibited <em>Aspergillus flavus, Candida albicans </em>and <em>Candida parapsilosis</em>. The non-fortified extracts however, had no inhibitory effects on the tested fungi. Based on the findings of this study, it can be concluded that Se-fortification increased the antibacterial activities of <em>Pleurotus</em> spp. These results therefore, suggest that Se-enriched mushrooms can be used as potential source of dietary supplements and nutraceuticals. Fortification of <em>Pleurotus </em>spp. with essential metals should be encouraged as this can improve its health promoting property. |
| first_indexed | 2024-12-12T05:09:15Z |
| format | Article |
| id | doaj.art-e28062e607bb4ba6bba7b15d6473a7d8 |
| institution | Directory Open Access Journal |
| issn | 2357-0326 2357-0334 |
| language | English |
| last_indexed | 2024-12-12T05:09:15Z |
| publishDate | 2018-12-01 |
| publisher | Arab Society for Fungal Conservation |
| record_format | Article |
| series | Microbial Biosystems Journal |
| spelling | doaj.art-e28062e607bb4ba6bba7b15d6473a7d82022-12-22T00:36:59ZengArab Society for Fungal ConservationMicrobial Biosystems Journal2357-03262357-03342018-12-0132293910.21608/mb.2018.2627426274Phytochemical constituents and antimicrobial evaluation of ethanolic extracts from Pleurotus spp. cultivated on substrate fortified with seleniumEdible mushrooms have the ability to bio-accumulate minerals from the growth medium into the fruiting body. Selenium (Se) is an example of minerals that can be easily absorbed by mushroom. This study investigated the phytochemical constituents and antimicrobial properties of <em>Pleurotus </em>spp. fortified with selenium. <em>Pleurotus</em> spp. (fortified with selenium and non-fortified with selenium) were cultivated on corn cobs using standard procedures. The yield, biological efficiency, selenium absorption level, phytochemical constituents and antimicrobial properties were determined using standard methods. Non-fortified <em>Pleurotus pulmonarius</em> recorded the highest yield and biological efficiency, while fortified <em>Pleurotus ostreatus </em>had the lowest yields and biological efficiency. There was no significant difference (P<0.05) in the selenium concentration absorbed in <em>Pleurotus ostreatus</em> (51.79µg/g) and <em>Pleurotus pulmonarius</em> (59.29 µg/g) but no selenium was detected in the non-fortified samples. The presence of 5 phytochemicals viz; saponins, alkaloids, phenols, tannins and flavonoids were detected in cultivated mushrooms. Saponin was highest (7.08 mg/g) in non-fortified <em>Pleurotus pulmonarius</em> while tannin was the least with the value of 0.02 mg/g in fortified <em>Pleurotus ostreatus</em>. The ethanolic extracts of <em>Pleurotus</em> spp. inhibited the growth of Gram positive and negative bacteria with <em>Pseudomonas aeruginosa</em>been the most susceptible (25.00 mm). The ethanolic extracts of fortified <em>Pleurotus </em>spp. inhibited <em>Aspergillus flavus, Candida albicans </em>and <em>Candida parapsilosis</em>. The non-fortified extracts however, had no inhibitory effects on the tested fungi. Based on the findings of this study, it can be concluded that Se-fortification increased the antibacterial activities of <em>Pleurotus</em> spp. These results therefore, suggest that Se-enriched mushrooms can be used as potential source of dietary supplements and nutraceuticals. Fortification of <em>Pleurotus </em>spp. with essential metals should be encouraged as this can improve its health promoting property.https://mb.journals.ekb.eg/article_26274_368eafea18edd45fbf2a2f4f32c133d9.pdfantimicrobialphytochemicalpleurotus speciesselenium |
| spellingShingle | Phytochemical constituents and antimicrobial evaluation of ethanolic extracts from Pleurotus spp. cultivated on substrate fortified with selenium Microbial Biosystems Journal antimicrobial phytochemical pleurotus species selenium |
| title | Phytochemical constituents and antimicrobial evaluation of ethanolic extracts from Pleurotus spp. cultivated on substrate fortified with selenium |
| title_full | Phytochemical constituents and antimicrobial evaluation of ethanolic extracts from Pleurotus spp. cultivated on substrate fortified with selenium |
| title_fullStr | Phytochemical constituents and antimicrobial evaluation of ethanolic extracts from Pleurotus spp. cultivated on substrate fortified with selenium |
| title_full_unstemmed | Phytochemical constituents and antimicrobial evaluation of ethanolic extracts from Pleurotus spp. cultivated on substrate fortified with selenium |
| title_short | Phytochemical constituents and antimicrobial evaluation of ethanolic extracts from Pleurotus spp. cultivated on substrate fortified with selenium |
| title_sort | phytochemical constituents and antimicrobial evaluation of ethanolic extracts from pleurotus spp cultivated on substrate fortified with selenium |
| topic | antimicrobial phytochemical pleurotus species selenium |
| url | https://mb.journals.ekb.eg/article_26274_368eafea18edd45fbf2a2f4f32c133d9.pdf |