Effect of Dietary Supplementation of <i>Lactobacillus Casei</i> YYL3 and <i>L. Plantarum</i> YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish
The purpose of this study is to investigate the effect of probiotics <i>L. casei</i> YYL3 (Lc) and <i>L. plantarum</i> YYL5 (Lp) on growth performance, innate immunity, disease resistance and intestinal microbiota of channel catfish. A total of 252 catfish (67.20 ± 1...
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| Format: | Article |
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MDPI AG
2019-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/12/1005 |
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| author | Hongyu Zhang Haibo Wang Kun Hu Liting Jiao Mingjun Zhao Xianle Yang Lei Xia |
| author_facet | Hongyu Zhang Haibo Wang Kun Hu Liting Jiao Mingjun Zhao Xianle Yang Lei Xia |
| author_sort | Hongyu Zhang |
| collection | DOAJ |
| description | The purpose of this study is to investigate the effect of probiotics <i>L. casei</i> YYL3 (Lc) and <i>L. plantarum</i> YYL5 (Lp) on growth performance, innate immunity, disease resistance and intestinal microbiota of channel catfish. A total of 252 catfish (67.20 ± 1.46 g) were randomly divided into 3 groups which were fed with basal diet, Lc-added (3.0 × 10<sup>8</sup> cfu/g) or Lp-added (3.0 × 10<sup>8</sup> cfu/g) diets, respectively. After 4 weeks of feeding, Lc significantly enhanced the growth and feed utilization of channel catfish compared with the control group (CG). Following that, the catfish were challenged with an intraperitoneal injection of 200 μL of the pathogenic <i>E.ictaluri</i> (2.0 × 10<sup>6</sup> cfu/mL), the relative percent survival of Lc and Lp were 38.28% and 12.76%, respectively. High-throughput sequencing indicated Lc and Lp reduced the alpha diversity of the intestinal microbiota in channel catfish. <i>Lactobacillus</i> were overwhelming in the guts during probiotics treatment, but almost vanished away after 2 weeks post-cessation of probiotics administration. Compared to CG, Lc and Lp resulted in an increased abundance of <i>Pseudomonas</i> and decreased amount of <i>Aeromonas</i>. Functional analysis revealed that Lc treatment upregulated the relative abundance of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including lipid metabolism, metabolism of other amino acids, metabolism of terpenoids and polyketides, xenobiotics biodegradation and metabolism, and nucleotide metabolism. Combined, our data revealed that Lc, as a feed additive at 3.0 × 10<sup>8</sup> cfu/g, could promote the growth performance, disease resistance and dramatically change the composition of intestinal microbiota of channel catfish. |
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| language | English |
| last_indexed | 2024-04-12T06:18:46Z |
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| spelling | doaj.art-e280ee5f82474ca1b099ac89beb9af9f2022-12-22T03:44:23ZengMDPI AGAnimals2076-26152019-11-01912100510.3390/ani9121005ani9121005Effect of Dietary Supplementation of <i>Lactobacillus Casei</i> YYL3 and <i>L. Plantarum</i> YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel CatfishHongyu Zhang0Haibo Wang1Kun Hu2Liting Jiao3Mingjun Zhao4Xianle Yang5Lei Xia6National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 210306, ChinaChinese Academy of Fishery Sciences, Beijing 100141, ChinaNational Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 210306, ChinaChinese Academy of Fishery Sciences, Beijing 100141, ChinaChinese Academy of Fishery Sciences, Beijing 100141, ChinaNational Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 210306, ChinaChinese Academy of Fishery Sciences, Beijing 100141, ChinaThe purpose of this study is to investigate the effect of probiotics <i>L. casei</i> YYL3 (Lc) and <i>L. plantarum</i> YYL5 (Lp) on growth performance, innate immunity, disease resistance and intestinal microbiota of channel catfish. A total of 252 catfish (67.20 ± 1.46 g) were randomly divided into 3 groups which were fed with basal diet, Lc-added (3.0 × 10<sup>8</sup> cfu/g) or Lp-added (3.0 × 10<sup>8</sup> cfu/g) diets, respectively. After 4 weeks of feeding, Lc significantly enhanced the growth and feed utilization of channel catfish compared with the control group (CG). Following that, the catfish were challenged with an intraperitoneal injection of 200 μL of the pathogenic <i>E.ictaluri</i> (2.0 × 10<sup>6</sup> cfu/mL), the relative percent survival of Lc and Lp were 38.28% and 12.76%, respectively. High-throughput sequencing indicated Lc and Lp reduced the alpha diversity of the intestinal microbiota in channel catfish. <i>Lactobacillus</i> were overwhelming in the guts during probiotics treatment, but almost vanished away after 2 weeks post-cessation of probiotics administration. Compared to CG, Lc and Lp resulted in an increased abundance of <i>Pseudomonas</i> and decreased amount of <i>Aeromonas</i>. Functional analysis revealed that Lc treatment upregulated the relative abundance of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including lipid metabolism, metabolism of other amino acids, metabolism of terpenoids and polyketides, xenobiotics biodegradation and metabolism, and nucleotide metabolism. Combined, our data revealed that Lc, as a feed additive at 3.0 × 10<sup>8</sup> cfu/g, could promote the growth performance, disease resistance and dramatically change the composition of intestinal microbiota of channel catfish.https://www.mdpi.com/2076-2615/9/12/1005channel catfish<i>l. casei</i><i>l. plantarum</i>growthdisease resistanceintestinal microbiota |
| spellingShingle | Hongyu Zhang Haibo Wang Kun Hu Liting Jiao Mingjun Zhao Xianle Yang Lei Xia Effect of Dietary Supplementation of <i>Lactobacillus Casei</i> YYL3 and <i>L. Plantarum</i> YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish Animals channel catfish <i>l. casei</i> <i>l. plantarum</i> growth disease resistance intestinal microbiota |
| title | Effect of Dietary Supplementation of <i>Lactobacillus Casei</i> YYL3 and <i>L. Plantarum</i> YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish |
| title_full | Effect of Dietary Supplementation of <i>Lactobacillus Casei</i> YYL3 and <i>L. Plantarum</i> YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish |
| title_fullStr | Effect of Dietary Supplementation of <i>Lactobacillus Casei</i> YYL3 and <i>L. Plantarum</i> YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish |
| title_full_unstemmed | Effect of Dietary Supplementation of <i>Lactobacillus Casei</i> YYL3 and <i>L. Plantarum</i> YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish |
| title_short | Effect of Dietary Supplementation of <i>Lactobacillus Casei</i> YYL3 and <i>L. Plantarum</i> YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish |
| title_sort | effect of dietary supplementation of i lactobacillus casei i yyl3 and i l plantarum i yyl5 on growth immune response and intestinal microbiota in channel catfish |
| topic | channel catfish <i>l. casei</i> <i>l. plantarum</i> growth disease resistance intestinal microbiota |
| url | https://www.mdpi.com/2076-2615/9/12/1005 |
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