Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i>
Acute hepatopancreas necrosis disease (AHPND) has caused massive deaths of shrimp and has led to huge economic losses in aquaculture. <i>Vibrio parahaemolyticus</i> (VP<sub>AHPND</sub>) carrying a plasmid encoding binary toxins homologous to the <i>photorhabdus</i>...
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MDPI AG
2023-03-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/3/417 |
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| author | Miao Miao Shihao Li Yuan Liu Yang Yu Fuhua Li |
| author_facet | Miao Miao Shihao Li Yuan Liu Yang Yu Fuhua Li |
| author_sort | Miao Miao |
| collection | DOAJ |
| description | Acute hepatopancreas necrosis disease (AHPND) has caused massive deaths of shrimp and has led to huge economic losses in aquaculture. <i>Vibrio parahaemolyticus</i> (VP<sub>AHPND</sub>) carrying a plasmid encoding binary toxins homologous to the <i>photorhabdus</i> insect-related (Pir) toxins is one of the main pathogens causing this disease. Previous studies have reported many immune-related genes of shrimp in response to this pathogenic bacteria. However, few studies have so far focused on the metabolic changes in <i>Litopenaeus vannamei</i> upon VP<sub>AHPND</sub> infection. In the present study, comparative transcriptomic analysis was performed on the hepatopancreas of shrimp at different times during VP<sub>AHPND</sub> infection. Functional analyses on the differentially expressed genes (DEGs) during infection showed that pathways related to glucose, energy and amino acid metabolism, as well as nucleic acid synthesis, were obviously changed in the hepatopancreas after VP<sub>AHPND</sub> infection. Additionally, three signaling pathways, which could regulate metabolic processes, including HIF-1 signaling pathway, PI3K-Akt signaling pathway and NF-KappaB signaling pathway, also changed significantly. Collectively, these data reveal a close relationship between host metabolism processes and <i>Vibrio</i> infection. The information will enrich our understanding of the interaction mechanism between the shrimp and <i>Vibrio</i>. |
| first_indexed | 2024-03-11T06:54:58Z |
| format | Article |
| id | doaj.art-e28197868355496eb90a093ca50cf48d |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T06:54:58Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
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| spelling | doaj.art-e28197868355496eb90a093ca50cf48d2023-11-17T09:41:55ZengMDPI AGBiology2079-77372023-03-0112341710.3390/biology12030417Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i>Miao Miao0Shihao Li1Yuan Liu2Yang Yu3Fuhua Li4CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaAcute hepatopancreas necrosis disease (AHPND) has caused massive deaths of shrimp and has led to huge economic losses in aquaculture. <i>Vibrio parahaemolyticus</i> (VP<sub>AHPND</sub>) carrying a plasmid encoding binary toxins homologous to the <i>photorhabdus</i> insect-related (Pir) toxins is one of the main pathogens causing this disease. Previous studies have reported many immune-related genes of shrimp in response to this pathogenic bacteria. However, few studies have so far focused on the metabolic changes in <i>Litopenaeus vannamei</i> upon VP<sub>AHPND</sub> infection. In the present study, comparative transcriptomic analysis was performed on the hepatopancreas of shrimp at different times during VP<sub>AHPND</sub> infection. Functional analyses on the differentially expressed genes (DEGs) during infection showed that pathways related to glucose, energy and amino acid metabolism, as well as nucleic acid synthesis, were obviously changed in the hepatopancreas after VP<sub>AHPND</sub> infection. Additionally, three signaling pathways, which could regulate metabolic processes, including HIF-1 signaling pathway, PI3K-Akt signaling pathway and NF-KappaB signaling pathway, also changed significantly. Collectively, these data reveal a close relationship between host metabolism processes and <i>Vibrio</i> infection. The information will enrich our understanding of the interaction mechanism between the shrimp and <i>Vibrio</i>.https://www.mdpi.com/2079-7737/12/3/417transcriptomicshepatopancreasmetabolism<i>Vibrio</i> infectionshrimp |
| spellingShingle | Miao Miao Shihao Li Yuan Liu Yang Yu Fuhua Li Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i> Biology transcriptomics hepatopancreas metabolism <i>Vibrio</i> infection shrimp |
| title | Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i> |
| title_full | Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i> |
| title_fullStr | Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i> |
| title_full_unstemmed | Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i> |
| title_short | Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by <i>Vibrio parahaemolyticus</i> Infection in <i>Litopenaeus vannamei</i> |
| title_sort | transcriptome analysis on hepatopancreas reveals the metabolic dysregulation caused by i vibrio parahaemolyticus i infection in i litopenaeus vannamei i |
| topic | transcriptomics hepatopancreas metabolism <i>Vibrio</i> infection shrimp |
| url | https://www.mdpi.com/2079-7737/12/3/417 |
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