Characterization and statistical optimization of biosurfactant production using Bacillus subtilis isolated from automotive oil-contaminated soil in Yola, Nigeria
The need for cost effective and ecofriendly means of producing compounds of environmental, industrial, and other biotechnological applications cannot be overemphasized. Biosurfactant from microbial sources have been identified to have the potential to substitute chemical types. This has led to the c...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227622002642 |
| _version_ | 1811344272360734720 |
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| author | Mohammed Inuwa Ja'afaru Tema Abbas Obinna Markraphael Ajunwa Kayode Olaifa |
| author_facet | Mohammed Inuwa Ja'afaru Tema Abbas Obinna Markraphael Ajunwa Kayode Olaifa |
| author_sort | Mohammed Inuwa Ja'afaru |
| collection | DOAJ |
| description | The need for cost effective and ecofriendly means of producing compounds of environmental, industrial, and other biotechnological applications cannot be overemphasized. Biosurfactant from microbial sources have been identified to have the potential to substitute chemical types. This has led to the continuous bioprospecting for biosurfactant-producing microorganisms and optimization of processes for high yield biosurfactant production. Despite the widespread, high levels of oil-induced soil pollution in Yola, Nigeria and its environs, there is dearth of information on the potential application of indigenous bacteria for biosurfactant production. In this study, a Bacillus subtilis B3 strain was isolated from an automotive oil-contaminated soil in Yola, and was further used for production, characterization, and optimization of biosurfactant activity. The partially purified biosurfactant had a structural elucidation of a lipopeptide and exhibited surface tension reduction of distilled water from 72 to 29.30 mN/m. It also forms stable emulsions between pH 6-8 and temperature 60-80 °C, and a critical micelle concentration of 0.65 %. The dry weight of the purified biosurfactant was 0.25 g/L representing a 1.6-fold increase in comparison with one obtained from conventional medium with 0.16 g/L biosurfactant yield. This finding suggests that the bacterial strain isolated in this study has the potential to meet the demand for bioremediation and other industrial exploitations. |
| first_indexed | 2024-04-13T19:44:50Z |
| format | Article |
| id | doaj.art-e282ec32e59148ffa747dabe28344e6a |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-04-13T19:44:50Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-e282ec32e59148ffa747dabe28344e6a2022-12-22T02:32:46ZengElsevierScientific African2468-22762022-09-0117e01357Characterization and statistical optimization of biosurfactant production using Bacillus subtilis isolated from automotive oil-contaminated soil in Yola, NigeriaMohammed Inuwa Ja'afaru0Tema Abbas1Obinna Markraphael Ajunwa2Kayode Olaifa3Department of Microbiology, Modibbo Adama University, P.M.B 2076 Yola, NigeriaDepartment of Microbiology, Modibbo Adama University, P.M.B 2076 Yola, NigeriaDepartment of Microbiology, Modibbo Adama University, P.M.B 2076 Yola, Nigeria; Biofilm Laboratory, School of Engineering and Digital Sciences, Nazarbayev University, 53 Kabanbay Batyr Avenue, Nur-Sultan 01000, Kazakhstan; Corresponding authorBiofilm Laboratory, School of Engineering and Digital Sciences, Nazarbayev University, 53 Kabanbay Batyr Avenue, Nur-Sultan 01000, KazakhstanThe need for cost effective and ecofriendly means of producing compounds of environmental, industrial, and other biotechnological applications cannot be overemphasized. Biosurfactant from microbial sources have been identified to have the potential to substitute chemical types. This has led to the continuous bioprospecting for biosurfactant-producing microorganisms and optimization of processes for high yield biosurfactant production. Despite the widespread, high levels of oil-induced soil pollution in Yola, Nigeria and its environs, there is dearth of information on the potential application of indigenous bacteria for biosurfactant production. In this study, a Bacillus subtilis B3 strain was isolated from an automotive oil-contaminated soil in Yola, and was further used for production, characterization, and optimization of biosurfactant activity. The partially purified biosurfactant had a structural elucidation of a lipopeptide and exhibited surface tension reduction of distilled water from 72 to 29.30 mN/m. It also forms stable emulsions between pH 6-8 and temperature 60-80 °C, and a critical micelle concentration of 0.65 %. The dry weight of the purified biosurfactant was 0.25 g/L representing a 1.6-fold increase in comparison with one obtained from conventional medium with 0.16 g/L biosurfactant yield. This finding suggests that the bacterial strain isolated in this study has the potential to meet the demand for bioremediation and other industrial exploitations.http://www.sciencedirect.com/science/article/pii/S2468227622002642Bacillus sppBiosurfactantOptimizationPollutionBiotechnological applications |
| spellingShingle | Mohammed Inuwa Ja'afaru Tema Abbas Obinna Markraphael Ajunwa Kayode Olaifa Characterization and statistical optimization of biosurfactant production using Bacillus subtilis isolated from automotive oil-contaminated soil in Yola, Nigeria Scientific African Bacillus spp Biosurfactant Optimization Pollution Biotechnological applications |
| title | Characterization and statistical optimization of biosurfactant production using Bacillus subtilis isolated from automotive oil-contaminated soil in Yola, Nigeria |
| title_full | Characterization and statistical optimization of biosurfactant production using Bacillus subtilis isolated from automotive oil-contaminated soil in Yola, Nigeria |
| title_fullStr | Characterization and statistical optimization of biosurfactant production using Bacillus subtilis isolated from automotive oil-contaminated soil in Yola, Nigeria |
| title_full_unstemmed | Characterization and statistical optimization of biosurfactant production using Bacillus subtilis isolated from automotive oil-contaminated soil in Yola, Nigeria |
| title_short | Characterization and statistical optimization of biosurfactant production using Bacillus subtilis isolated from automotive oil-contaminated soil in Yola, Nigeria |
| title_sort | characterization and statistical optimization of biosurfactant production using bacillus subtilis isolated from automotive oil contaminated soil in yola nigeria |
| topic | Bacillus spp Biosurfactant Optimization Pollution Biotechnological applications |
| url | http://www.sciencedirect.com/science/article/pii/S2468227622002642 |
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