Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas
Assessment of enzymatic characterization of Fusarium spp. isolated from human, animal, and plant wounds. Objective. Todetermine amylolytic, cellulolytic, lipolytic, pectinolytic and proteolytic activities in 32 Fusarium spp. isolates from humans, animals andplants. Materials and methods. Qualitative...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pontificia Universidad Javeriana
2011-08-01
|
| Series: | Universitas Scientiarum |
| Subjects: | |
| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1646/1073 |
| _version_ | 1819016564276461568 |
|---|---|
| author | María Ximena Rodríguez-Bocanegra Hugo Díez-Ortega Claudia Marcela Parra-Giraldo Marcela Franco-Correa Balkys Quevedo-Hidalgo María Fernanda Valencia-Guerrero |
| author_facet | María Ximena Rodríguez-Bocanegra Hugo Díez-Ortega Claudia Marcela Parra-Giraldo Marcela Franco-Correa Balkys Quevedo-Hidalgo María Fernanda Valencia-Guerrero |
| author_sort | María Ximena Rodríguez-Bocanegra |
| collection | DOAJ |
| description | Assessment of enzymatic characterization of Fusarium spp. isolated from human, animal, and plant wounds. Objective. Todetermine amylolytic, cellulolytic, lipolytic, pectinolytic and proteolytic activities in 32 Fusarium spp. isolates from humans, animals andplants. Materials and methods. Qualitative determination of enzymatic activities was done by measuring hydrolysis halos in agar plateswith their corresponding substrate. Quantitative determination was done by colorimetric techniques, using liquid culture supernatantsto determine the respective substrate degradation. Results. All isolates showed enzymatic activities from a qualitative point of view,except amylolytic and lipolytic. Quantitative determination was possible for all the evaluated enzymes except lipases. Conclusion.The determination of amylolytic, cellulolytic, pectinolytic and proteolytic enzymatic profiles of each of the Fusarium isolates assessed suggests their capacity to degrade these substrates, irrespectively of their origin. |
| first_indexed | 2024-12-21T02:49:38Z |
| format | Article |
| id | doaj.art-e2830b58d07f46d090356b86d7f2abfe |
| institution | Directory Open Access Journal |
| issn | 0122-7483 2027-1352 |
| language | English |
| last_indexed | 2024-12-21T02:49:38Z |
| publishDate | 2011-08-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj.art-e2830b58d07f46d090356b86d7f2abfe2022-12-21T19:18:28ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522011-08-01162147159Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantasMaría Ximena Rodríguez-BocanegraHugo Díez-OrtegaClaudia Marcela Parra-GiraldoMarcela Franco-CorreaBalkys Quevedo-HidalgoMaría Fernanda Valencia-GuerreroAssessment of enzymatic characterization of Fusarium spp. isolated from human, animal, and plant wounds. Objective. Todetermine amylolytic, cellulolytic, lipolytic, pectinolytic and proteolytic activities in 32 Fusarium spp. isolates from humans, animals andplants. Materials and methods. Qualitative determination of enzymatic activities was done by measuring hydrolysis halos in agar plateswith their corresponding substrate. Quantitative determination was done by colorimetric techniques, using liquid culture supernatantsto determine the respective substrate degradation. Results. All isolates showed enzymatic activities from a qualitative point of view,except amylolytic and lipolytic. Quantitative determination was possible for all the evaluated enzymes except lipases. Conclusion.The determination of amylolytic, cellulolytic, pectinolytic and proteolytic enzymatic profiles of each of the Fusarium isolates assessed suggests their capacity to degrade these substrates, irrespectively of their origin.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1646/1073Fusariummultihost pathogenamylasescellulosespectinasesproteaseslipases |
| spellingShingle | María Ximena Rodríguez-Bocanegra Hugo Díez-Ortega Claudia Marcela Parra-Giraldo Marcela Franco-Correa Balkys Quevedo-Hidalgo María Fernanda Valencia-Guerrero Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas Universitas Scientiarum Fusarium multihost pathogen amylases celluloses pectinases proteases lipases |
| title | Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas |
| title_full | Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas |
| title_fullStr | Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas |
| title_full_unstemmed | Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas |
| title_short | Evaluación de actividades enzimáticas de Fusarium spp., aislados de lesiones en humanos, animales y plantas |
| title_sort | evaluacion de actividades enzimaticas de fusarium spp aislados de lesiones en humanos animales y plantas |
| topic | Fusarium multihost pathogen amylases celluloses pectinases proteases lipases |
| url | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1646/1073 |
| work_keys_str_mv | AT mariaximenarodriguezbocanegra evaluaciondeactividadesenzimaticasdefusariumsppaisladosdelesionesenhumanosanimalesyplantas AT hugodiezortega evaluaciondeactividadesenzimaticasdefusariumsppaisladosdelesionesenhumanosanimalesyplantas AT claudiamarcelaparragiraldo evaluaciondeactividadesenzimaticasdefusariumsppaisladosdelesionesenhumanosanimalesyplantas AT marcelafrancocorrea evaluaciondeactividadesenzimaticasdefusariumsppaisladosdelesionesenhumanosanimalesyplantas AT balkysquevedohidalgo evaluaciondeactividadesenzimaticasdefusariumsppaisladosdelesionesenhumanosanimalesyplantas AT mariafernandavalenciaguerrero evaluaciondeactividadesenzimaticasdefusariumsppaisladosdelesionesenhumanosanimalesyplantas |