Innovative Purification Method of Ovatodiolide from <i>Anisomeles indica</i> to Induce Apoptosis in Human Gastric Cancer Cells
Ovatodiolide (Ova), found in the plant <i>Anisomeles indica</i> (AI), has been reported to have an anti-proliferation effect in various cancer cells. However, little information is available regarding the anti-cancer effect of Ova in human gastric cancer cells. In this study, we investig...
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/3/587 |
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| author | Hsiu-Man Lien Shiau-Huei Huang Chi-Huang Chang Chao-Lu Huang Chia-Chang Chen Charng-Cherng Chyau |
| author_facet | Hsiu-Man Lien Shiau-Huei Huang Chi-Huang Chang Chao-Lu Huang Chia-Chang Chen Charng-Cherng Chyau |
| author_sort | Hsiu-Man Lien |
| collection | DOAJ |
| description | Ovatodiolide (Ova), found in the plant <i>Anisomeles indica</i> (AI), has been reported to have an anti-proliferation effect in various cancer cells. However, little information is available regarding the anti-cancer effect of Ova in human gastric cancer cells. In this study, we investigated the inhibitory effects and the mechanisms of action responsible for these effects on human AGS cell lines from a newly developed purification technique for Ova from AI extract. Extract obtained at the optimum condition of 95% ethanol extraction of AI was sequentially partitioned by using different polarity solvents. Enriched content of Ova (35.9% purity) from the <i>n</i>-hexane fraction was then applied to the purification by using centrifugal partition chromatography (CPC) in a two-phase solvent system consisting of <i>n</i>-hexane:ethyl acetate:methanol:water (1.0:1.0:1.0:1.0, <i>v</i>/<i>v</i>/<i>v</i>/<i>v</i>) to reach purity over >95.0%. In evaluation of the anti-proliferation effect on AGS cells, Ova induced cell apoptosis with IC<sub>50</sub> values of 13.02 and 6.18 μM at 24 and 48 h, respectively, and arrested the cells at the G2/M phase. Quantification of Bax/Bcl2 mRNA expressions using qPCR showed a 2.5-fold increase in the Ova (5 μM)-treated cells at 48 h than in the control group. Specific protein expression data warrant further research to further confirm the proposed Ova-induced apoptotic pathway in AGS cells. |
| first_indexed | 2024-03-09T23:31:38Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:31:38Z |
| publishDate | 2022-01-01 |
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| series | Molecules |
| spelling | doaj.art-e2848d28c0e54f3a9944ee425719b40f2023-11-23T17:09:10ZengMDPI AGMolecules1420-30492022-01-0127358710.3390/molecules27030587Innovative Purification Method of Ovatodiolide from <i>Anisomeles indica</i> to Induce Apoptosis in Human Gastric Cancer CellsHsiu-Man Lien0Shiau-Huei Huang1Chi-Huang Chang2Chao-Lu Huang3Chia-Chang Chen4Charng-Cherng Chyau5Research Institute of Biotechnology, Hungkuang University, Shalu District, Taichung 43302, TaiwanResearch Institute of Biotechnology, Hungkuang University, Shalu District, Taichung 43302, TaiwanResearch Institute of Biotechnology, Hungkuang University, Shalu District, Taichung 43302, TaiwanYusheng Biotechnology Center, No. 26, Keyuan Rd., Xitun District, Taichung 40763, TaiwanYusheng Biotechnology Center, No. 26, Keyuan Rd., Xitun District, Taichung 40763, TaiwanResearch Institute of Biotechnology, Hungkuang University, Shalu District, Taichung 43302, TaiwanOvatodiolide (Ova), found in the plant <i>Anisomeles indica</i> (AI), has been reported to have an anti-proliferation effect in various cancer cells. However, little information is available regarding the anti-cancer effect of Ova in human gastric cancer cells. In this study, we investigated the inhibitory effects and the mechanisms of action responsible for these effects on human AGS cell lines from a newly developed purification technique for Ova from AI extract. Extract obtained at the optimum condition of 95% ethanol extraction of AI was sequentially partitioned by using different polarity solvents. Enriched content of Ova (35.9% purity) from the <i>n</i>-hexane fraction was then applied to the purification by using centrifugal partition chromatography (CPC) in a two-phase solvent system consisting of <i>n</i>-hexane:ethyl acetate:methanol:water (1.0:1.0:1.0:1.0, <i>v</i>/<i>v</i>/<i>v</i>/<i>v</i>) to reach purity over >95.0%. In evaluation of the anti-proliferation effect on AGS cells, Ova induced cell apoptosis with IC<sub>50</sub> values of 13.02 and 6.18 μM at 24 and 48 h, respectively, and arrested the cells at the G2/M phase. Quantification of Bax/Bcl2 mRNA expressions using qPCR showed a 2.5-fold increase in the Ova (5 μM)-treated cells at 48 h than in the control group. Specific protein expression data warrant further research to further confirm the proposed Ova-induced apoptotic pathway in AGS cells.https://www.mdpi.com/1420-3049/27/3/587<i>Anisomeles indica</i>ovatodiolidecentrifugal partition chromatographyhuman gastric cancer cell line AGSapoptosis |
| spellingShingle | Hsiu-Man Lien Shiau-Huei Huang Chi-Huang Chang Chao-Lu Huang Chia-Chang Chen Charng-Cherng Chyau Innovative Purification Method of Ovatodiolide from <i>Anisomeles indica</i> to Induce Apoptosis in Human Gastric Cancer Cells Molecules <i>Anisomeles indica</i> ovatodiolide centrifugal partition chromatography human gastric cancer cell line AGS apoptosis |
| title | Innovative Purification Method of Ovatodiolide from <i>Anisomeles indica</i> to Induce Apoptosis in Human Gastric Cancer Cells |
| title_full | Innovative Purification Method of Ovatodiolide from <i>Anisomeles indica</i> to Induce Apoptosis in Human Gastric Cancer Cells |
| title_fullStr | Innovative Purification Method of Ovatodiolide from <i>Anisomeles indica</i> to Induce Apoptosis in Human Gastric Cancer Cells |
| title_full_unstemmed | Innovative Purification Method of Ovatodiolide from <i>Anisomeles indica</i> to Induce Apoptosis in Human Gastric Cancer Cells |
| title_short | Innovative Purification Method of Ovatodiolide from <i>Anisomeles indica</i> to Induce Apoptosis in Human Gastric Cancer Cells |
| title_sort | innovative purification method of ovatodiolide from i anisomeles indica i to induce apoptosis in human gastric cancer cells |
| topic | <i>Anisomeles indica</i> ovatodiolide centrifugal partition chromatography human gastric cancer cell line AGS apoptosis |
| url | https://www.mdpi.com/1420-3049/27/3/587 |
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