Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation
Postmenopausal osteoporosis (PMOP) is a systemic metabolic bone disease in postmenopausal women. It has been known that long non-coding RNAs (lncRNAs) play a regulatory role in the progression of osteoporosis. However, the mechanism underlying the effects of exosome-derived lncRNA on regulating the...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.831420/full |
| _version_ | 1818907568907485184 |
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| author | Yongchang Yang Yongchang Yang Li Miao Shuai Chang Qiuli Zhang Lijuan Yu Lijuan Yu Ping He Yue Zhang Yue Zhang Weixiao Fan Weixiao Fan Jie Liu Xiaoke Hao Xiaoke Hao Xiaoke Hao |
| author_facet | Yongchang Yang Yongchang Yang Li Miao Shuai Chang Qiuli Zhang Lijuan Yu Lijuan Yu Ping He Yue Zhang Yue Zhang Weixiao Fan Weixiao Fan Jie Liu Xiaoke Hao Xiaoke Hao Xiaoke Hao |
| author_sort | Yongchang Yang |
| collection | DOAJ |
| description | Postmenopausal osteoporosis (PMOP) is a systemic metabolic bone disease in postmenopausal women. It has been known that long non-coding RNAs (lncRNAs) play a regulatory role in the progression of osteoporosis. However, the mechanism underlying the effects of exosome-derived lncRNA on regulating the occurrence and development of PMOP remains unclear. Exosomes in the serum of patients PMOP were collected and identified. RNA sequencing was performed to obtain the expression profile of exosome-derived lncRNAs in the serum of PMOP patients. RNA sequencing identified 26 differentially expressed lncRNAs from the exosomes between healthy people and PMOP patients. Among them, the expression of TCONS_00072128 was dramatically down-regulated. A co-location method was employed and searched its potential target gene caspase 8. TCONS_00072128 knockdown notably decreased the expression of caspase 8, while the osteogenic differentiation of BMSCs was also reduced. Reversely, TCONS_00072128 overexpression enhanced caspase 8 expression and osteogenic differentiation of BMSCs. Moreover, the continuous expression of caspase 8 regulated by TCONS_00072128 significantly activated inflammation pathways including NLRP3 signaling and NF-κB signaling. Simultaneously, RIPK1 which has emerged as a promising therapeutic target for the treatment of a wide range of human neurodegenerative, autoimmune, and inflammatory diseases, was also phosphorylated. The results of the present study suggested that exosome-derived lncRNA TCONS_00072128 could promote the progression of PMOP by regulating caspase 8. In addition, caspase 8 expression in BMSCs was possible to be a key regulator that balanced cell differentiation and inflammation activation. |
| first_indexed | 2024-12-19T21:57:12Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-19T21:57:12Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-e284acd3da9945099e82a67c755c9c222022-12-21T20:04:15ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-03-011210.3389/fgene.2021.831420831420Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 RegulationYongchang Yang0Yongchang Yang1Li Miao2Shuai Chang3Qiuli Zhang4Lijuan Yu5Lijuan Yu6Ping He7Yue Zhang8Yue Zhang9Weixiao Fan10Weixiao Fan11Jie Liu12Xiaoke Hao13Xiaoke Hao14Xiaoke Hao15Institute of Laboratory Medicine Center of Chinese People’s Liberation Army (PLA), Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi’an, ChinaDepartment of Clinical Laboratory, Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Stomatology, Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Clinical Laboratory, Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Blood Transfusion, Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaInstitute of Laboratory Medicine Center of Chinese People’s Liberation Army (PLA), Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi’an, ChinaDepartment of Clinical Laboratory Medicine, Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi’an, ChinaBMD Testing Room, Department of Orthopedic, Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaInstitute of Laboratory Medicine Center of Chinese People’s Liberation Army (PLA), Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi’an, ChinaDepartment of Clinical Laboratory, Air Force Hospital in the Northern Theater Command, Shenyang, ChinaInstitute of Laboratory Medicine Center of Chinese People’s Liberation Army (PLA), Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi’an, ChinaDepartment of Clinical Laboratory Medicine, Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi’an, ChinaDepartment of Clinical Laboratory, Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaInstitute of Laboratory Medicine Center of Chinese People’s Liberation Army (PLA), Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi’an, ChinaDepartment of Clinical Laboratory Medicine, Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi’an, ChinaCollege of Medicine, Northwest University, Xi’an, ChinaPostmenopausal osteoporosis (PMOP) is a systemic metabolic bone disease in postmenopausal women. It has been known that long non-coding RNAs (lncRNAs) play a regulatory role in the progression of osteoporosis. However, the mechanism underlying the effects of exosome-derived lncRNA on regulating the occurrence and development of PMOP remains unclear. Exosomes in the serum of patients PMOP were collected and identified. RNA sequencing was performed to obtain the expression profile of exosome-derived lncRNAs in the serum of PMOP patients. RNA sequencing identified 26 differentially expressed lncRNAs from the exosomes between healthy people and PMOP patients. Among them, the expression of TCONS_00072128 was dramatically down-regulated. A co-location method was employed and searched its potential target gene caspase 8. TCONS_00072128 knockdown notably decreased the expression of caspase 8, while the osteogenic differentiation of BMSCs was also reduced. Reversely, TCONS_00072128 overexpression enhanced caspase 8 expression and osteogenic differentiation of BMSCs. Moreover, the continuous expression of caspase 8 regulated by TCONS_00072128 significantly activated inflammation pathways including NLRP3 signaling and NF-κB signaling. Simultaneously, RIPK1 which has emerged as a promising therapeutic target for the treatment of a wide range of human neurodegenerative, autoimmune, and inflammatory diseases, was also phosphorylated. The results of the present study suggested that exosome-derived lncRNA TCONS_00072128 could promote the progression of PMOP by regulating caspase 8. In addition, caspase 8 expression in BMSCs was possible to be a key regulator that balanced cell differentiation and inflammation activation.https://www.frontiersin.org/articles/10.3389/fgene.2021.831420/fulllncRNAexosomecaspase 8osteogenic differentiationPMOP |
| spellingShingle | Yongchang Yang Yongchang Yang Li Miao Shuai Chang Qiuli Zhang Lijuan Yu Lijuan Yu Ping He Yue Zhang Yue Zhang Weixiao Fan Weixiao Fan Jie Liu Xiaoke Hao Xiaoke Hao Xiaoke Hao Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation Frontiers in Genetics lncRNA exosome caspase 8 osteogenic differentiation PMOP |
| title | Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation |
| title_full | Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation |
| title_fullStr | Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation |
| title_full_unstemmed | Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation |
| title_short | Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation |
| title_sort | exosome derived lncrna tcons 00072128 mediated osteogenic differentiation and inflammation by caspase 8 regulation |
| topic | lncRNA exosome caspase 8 osteogenic differentiation PMOP |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.831420/full |
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