Commercial gouache as a dye for termites in laboratory assays

Studies were performed to evaluate the suitability of a commercial gouache as an external marker for termite workers in lab assays. Dyed and non-dyed termites presented similar survival for the first 300 minutes since the experiment has began, and differed after this time. Mortality did not differ f...

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Main Authors: Guilherme S. Brunow, Og de Souza, Octavio Miramontes
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2005-07-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500010
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author Guilherme S. Brunow
Og de Souza
Octavio Miramontes
author_facet Guilherme S. Brunow
Og de Souza
Octavio Miramontes
author_sort Guilherme S. Brunow
collection DOAJ
description Studies were performed to evaluate the suitability of a commercial gouache as an external marker for termite workers in lab assays. Dyed and non-dyed termites presented similar survival for the first 300 minutes since the experiment has began, and differed after this time. Mortality did not differ for termites dyed in two different colours from the same brand of gouache. It was concluded that commercial gouache could be a suitable marker for Cornitermes cumulans (Isoptera: Termitidae) termites, provided following restrictions were applied (i) tested hypotheses did not rely on times to death greater than 300 min., or (ii) experiments included "sham-dyed" termites (i. e., a "control" group with "dyed" individuals, rather than the tradicional control group composed by non-dyed individuals).<br>Estudos foram conduzidos para avaliar a adequabilidade de um corante barato, prontamente disponível e aplicável topicamente, como marcador para operários de Cornitermes cumulans (Insecta: Isoptera) em ensaios de laboratório. Os padrões de sobrevivência dos cupins marcados não se distinguem daqueles apresentados pelos cupins sem marcação, cerca de 300 minutos desde o início do experimento. Após esse limiar, a mortalidade dos cupins marcados é acelerada quando comparada com cupins não marcados. Tal mortalidade, entretanto, não difere entre cupins marcados com duas cores diferentes da mesma marca comercial de guache. Concluiu-se que o guache comercial é adequado como marcador de operários de C. cumulans, desde que (i) experimentos não investiguem hipóteses explicitamente dependentes de tempos de sobrevivência maiores do que 300 min., ou então que (ii) experimentos incluam um grupo "controle" composto de cupins corados com um pigmento diferente da cor usada no grupo "tratamento".
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spelling doaj.art-e2880357de244866ad18925823b7af252022-12-22T01:08:08ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242005-07-0148457557910.1590/S1516-89132005000500010Commercial gouache as a dye for termites in laboratory assaysGuilherme S. BrunowOg de SouzaOctavio MiramontesStudies were performed to evaluate the suitability of a commercial gouache as an external marker for termite workers in lab assays. Dyed and non-dyed termites presented similar survival for the first 300 minutes since the experiment has began, and differed after this time. Mortality did not differ for termites dyed in two different colours from the same brand of gouache. It was concluded that commercial gouache could be a suitable marker for Cornitermes cumulans (Isoptera: Termitidae) termites, provided following restrictions were applied (i) tested hypotheses did not rely on times to death greater than 300 min., or (ii) experiments included "sham-dyed" termites (i. e., a "control" group with "dyed" individuals, rather than the tradicional control group composed by non-dyed individuals).<br>Estudos foram conduzidos para avaliar a adequabilidade de um corante barato, prontamente disponível e aplicável topicamente, como marcador para operários de Cornitermes cumulans (Insecta: Isoptera) em ensaios de laboratório. Os padrões de sobrevivência dos cupins marcados não se distinguem daqueles apresentados pelos cupins sem marcação, cerca de 300 minutos desde o início do experimento. Após esse limiar, a mortalidade dos cupins marcados é acelerada quando comparada com cupins não marcados. Tal mortalidade, entretanto, não difere entre cupins marcados com duas cores diferentes da mesma marca comercial de guache. Concluiu-se que o guache comercial é adequado como marcador de operários de C. cumulans, desde que (i) experimentos não investiguem hipóteses explicitamente dependentes de tempos de sobrevivência maiores do que 300 min., ou então que (ii) experimentos incluam um grupo "controle" composto de cupins corados com um pigmento diferente da cor usada no grupo "tratamento".http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500010biological dyesbehaviourlab assaysIsoptera
spellingShingle Guilherme S. Brunow
Og de Souza
Octavio Miramontes
Commercial gouache as a dye for termites in laboratory assays
Brazilian Archives of Biology and Technology
biological dyes
behaviour
lab assays
Isoptera
title Commercial gouache as a dye for termites in laboratory assays
title_full Commercial gouache as a dye for termites in laboratory assays
title_fullStr Commercial gouache as a dye for termites in laboratory assays
title_full_unstemmed Commercial gouache as a dye for termites in laboratory assays
title_short Commercial gouache as a dye for termites in laboratory assays
title_sort commercial gouache as a dye for termites in laboratory assays
topic biological dyes
behaviour
lab assays
Isoptera
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500010
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