4.2 SEX-DEPENDENT EFFECTS OF PERIVASCULAR ADIPOSE TISSUE ON VASCULAR FUNCTION
Background: Premenopausal women are relatively protected against hypertension compared to males. Estrogen levels have been identified as a potential underlying cause, but the pathophysiological mechanisms remain incompletely understood. We hypothesised that sex-dependent effects of perivascular adip...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-12-01
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| Series: | Artery Research |
| Online Access: | https://www.atlantis-press.com/article/125930202/view |
| Summary: | Background: Premenopausal women are relatively protected against hypertension compared to males. Estrogen levels have been identified as a potential underlying cause, but the pathophysiological mechanisms remain incompletely understood. We hypothesised that sex-dependent effects of perivascular adipose tissue PVAT mediate altered vascular function in hypertension.
Methods: The effect of PVAT was investigated on resistance vessels of 16 week old male and female stroke-prone spontaneously hypertensive rats (SHRSP).
Results: Wire-myography was used on 3rd-order mesenteric vessels (maximum contraction: male +PVAT 113.3 ± 1.1 vs. female +PVAT 91.4 ± 11.36 %). Noradrenaline mediated vasoconstriction was increased in SHRSP males compared to females. KATP channel-mediated vasorelaxation by cromakalim was impaired in males compared to females (maximum relaxation: male +PVAT 46.9 ± 3.9 % vs. female +PVAT 97.3 ± 2.7 %) A cross-over study assessing function of male PVAT on female vessels and vice versa confirmed the reduced KATP mediated vasorelaxation induced by male PVAT (maximum relaxation: female +PVATfemale 90.6 ± 1.4 % vs. female +PVATmale 65.8 ± 3.5 %). An adipokine array with subsequent western blot validation identified resistin as a potential modifier of vascular reactivity. Resistin was increased by approximately 2-fold in SHRSP male PVAT. Male and female vessels pretreated with resistin (40 ng/ml) showed no difference in response to noradrenaline. However, vasorelaxation in response to cromakalim was significantly impaired in resistin treated female vessels, similar to levels observed in male vessels (maximum relaxation: female +PVAT 97.3 ± 0.9 % vs. female +PVAT +resistin 36.8 ± 2.3 %).
Conclusion: We identified a novel role for resistin in sex-dependent PVAT mediated vascular function in hypertension through a KATP channel mediated mechanism. |
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| ISSN: | 1876-4401 |