Macroarray expression analysis of cytokines and prostaglandin metabolism–related genes in chronic rhinosinusitis
Background: Chronic rhinosinusitis (CRS) can be divided into endotypes by functional or pathophysiologic findings. Objective: The aim of this study was to analyze the expression of cytokines, prostaglandin (PG) synthases, and their receptors related to the pathogenesis of CRS, especially those contr...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Allergy and Clinical Immunology: Global |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772829323000486 |
| _version_ | 1797746007166943232 |
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| author | Aiko Oka, MD Kengo Kanai, MD Takaya Higaki, MD Seiichiro Makihara, MD Yohei Noda, MD Shin Kariya, MD Mizuo Ando, MD Wataru Nishimura, MD Mitsuhiro Okano, MD |
| author_facet | Aiko Oka, MD Kengo Kanai, MD Takaya Higaki, MD Seiichiro Makihara, MD Yohei Noda, MD Shin Kariya, MD Mizuo Ando, MD Wataru Nishimura, MD Mitsuhiro Okano, MD |
| author_sort | Aiko Oka, MD |
| collection | DOAJ |
| description | Background: Chronic rhinosinusitis (CRS) can be divided into endotypes by functional or pathophysiologic findings. Objective: The aim of this study was to analyze the expression of cytokines, prostaglandin (PG) synthases, and their receptors related to the pathogenesis of CRS, especially those contributing to nasal polyp (NP) formation. Methods: NPs and uncinate tissue (UT) samples were collected from 90 patients who underwent endoscopic sinus surgery. They included 75 patients with CRS (including 45 with eosinophilic CRS [eCRS] and 30 with non-eCRS) and 15 patients without CRS. A total of 30 genes were selected for our original DNA array plate to analyze the levels of expression of 10 cytokines (IFN-γ, IL-4, IL-5, IL-10, IL-13, IL-17A, IL-22, IL-25, IL-33, and TSLP), 4 prostaglandin synthases (prostaglandin D2 [PGD2] synthase, prostaglandin E2 synthase, COX-1, and COX-2), and their 16 receptors. Clustering analysis was performed according to the expression results, and clinical findings of patients from each cluster were investigated. Results: The samples could be divided into 3 clusters. Cluster 1 showed elevated levels of expression of IL4, IL5, IL13, TSLP, IL1RL1 (ST2 [an IL-33 receptor]), HPGDS, and GPR44 (CRTH2, a PGD2 receptor); cluster 2 showed elevated levels of expression of IL17A and PTGES; and cluster 3 showed an elevated level of expression of IL25. Regarding clinical features, the main characteristics of each cluster were as follows: NPs from patients with eCRS for cluster 1, NPs and/or UT samples from patients with non-eCRS for cluster 2, and UTs from patients with non-CRS for cluster 3. Conclusion: The results suggest that there are associations between type 2 inflammation/PGD2 and eCRS and also between type 3 inflammation/prostaglandin E2 and non-eCRS. |
| first_indexed | 2024-03-12T15:30:57Z |
| format | Article |
| id | doaj.art-e28c459f86d0486fa82a578bbed0072b |
| institution | Directory Open Access Journal |
| issn | 2772-8293 |
| language | English |
| last_indexed | 2024-03-12T15:30:57Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Allergy and Clinical Immunology: Global |
| spelling | doaj.art-e28c459f86d0486fa82a578bbed0072b2023-08-10T04:35:11ZengElsevierJournal of Allergy and Clinical Immunology: Global2772-82932023-08-0123100123Macroarray expression analysis of cytokines and prostaglandin metabolism–related genes in chronic rhinosinusitisAiko Oka, MD0Kengo Kanai, MD1Takaya Higaki, MD2Seiichiro Makihara, MD3Yohei Noda, MD4Shin Kariya, MD5Mizuo Ando, MD6Wataru Nishimura, MD7Mitsuhiro Okano, MD8Department of Otorhinolaryngology, International University of Health and Welfare School of Medicine, Narita, Japan; Corresponding author: Aiko Oka, MD, 852 Hatakeda, Narita, Chiba Prefecture 286-8520, Japan.Department of Otorhinolaryngology, International University of Health and Welfare School of Medicine, Narita, JapanDepartment of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, JapanDepartment of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, JapanDepartment of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, JapanDepartment of Otorhinolaryngology, International University of Health and Welfare School of Medicine, Narita, Japan; Department of Molecular Biology, International University of Health and Welfare School of Medicine, Narita, Japan; Department of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan; Department of Otolaryngology-Head and Neck Surgery, Kawasaki Medical School, Okayama, JapanDepartment of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, JapanDepartment of Molecular Biology, International University of Health and Welfare School of Medicine, Narita, JapanDepartment of Otorhinolaryngology, International University of Health and Welfare School of Medicine, Narita, JapanBackground: Chronic rhinosinusitis (CRS) can be divided into endotypes by functional or pathophysiologic findings. Objective: The aim of this study was to analyze the expression of cytokines, prostaglandin (PG) synthases, and their receptors related to the pathogenesis of CRS, especially those contributing to nasal polyp (NP) formation. Methods: NPs and uncinate tissue (UT) samples were collected from 90 patients who underwent endoscopic sinus surgery. They included 75 patients with CRS (including 45 with eosinophilic CRS [eCRS] and 30 with non-eCRS) and 15 patients without CRS. A total of 30 genes were selected for our original DNA array plate to analyze the levels of expression of 10 cytokines (IFN-γ, IL-4, IL-5, IL-10, IL-13, IL-17A, IL-22, IL-25, IL-33, and TSLP), 4 prostaglandin synthases (prostaglandin D2 [PGD2] synthase, prostaglandin E2 synthase, COX-1, and COX-2), and their 16 receptors. Clustering analysis was performed according to the expression results, and clinical findings of patients from each cluster were investigated. Results: The samples could be divided into 3 clusters. Cluster 1 showed elevated levels of expression of IL4, IL5, IL13, TSLP, IL1RL1 (ST2 [an IL-33 receptor]), HPGDS, and GPR44 (CRTH2, a PGD2 receptor); cluster 2 showed elevated levels of expression of IL17A and PTGES; and cluster 3 showed an elevated level of expression of IL25. Regarding clinical features, the main characteristics of each cluster were as follows: NPs from patients with eCRS for cluster 1, NPs and/or UT samples from patients with non-eCRS for cluster 2, and UTs from patients with non-CRS for cluster 3. Conclusion: The results suggest that there are associations between type 2 inflammation/PGD2 and eCRS and also between type 3 inflammation/prostaglandin E2 and non-eCRS.http://www.sciencedirect.com/science/article/pii/S2772829323000486Chronic rhinosinusitisendotypingprostaglandincytokinereceptorclustering analysis |
| spellingShingle | Aiko Oka, MD Kengo Kanai, MD Takaya Higaki, MD Seiichiro Makihara, MD Yohei Noda, MD Shin Kariya, MD Mizuo Ando, MD Wataru Nishimura, MD Mitsuhiro Okano, MD Macroarray expression analysis of cytokines and prostaglandin metabolism–related genes in chronic rhinosinusitis Journal of Allergy and Clinical Immunology: Global Chronic rhinosinusitis endotyping prostaglandin cytokine receptor clustering analysis |
| title | Macroarray expression analysis of cytokines and prostaglandin metabolism–related genes in chronic rhinosinusitis |
| title_full | Macroarray expression analysis of cytokines and prostaglandin metabolism–related genes in chronic rhinosinusitis |
| title_fullStr | Macroarray expression analysis of cytokines and prostaglandin metabolism–related genes in chronic rhinosinusitis |
| title_full_unstemmed | Macroarray expression analysis of cytokines and prostaglandin metabolism–related genes in chronic rhinosinusitis |
| title_short | Macroarray expression analysis of cytokines and prostaglandin metabolism–related genes in chronic rhinosinusitis |
| title_sort | macroarray expression analysis of cytokines and prostaglandin metabolism related genes in chronic rhinosinusitis |
| topic | Chronic rhinosinusitis endotyping prostaglandin cytokine receptor clustering analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2772829323000486 |
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