Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs
Endothelial progenitor cells (EPCs) expressing vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) and bone marrow mesenchymal stem cells (BMSCs) expressing endogenous bone morphogenetic protein-2 (BMP-2) play the important role in new bone formation. This study inves...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.928041/full |
| _version_ | 1828518055695613952 |
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| author | Hedong Yu Hedong Yu Lingyun Xia Lingyun Xia Xieyuan Leng Yongji Chen Yongji Chen Li Zhang Li Zhang Xiaobing Ni Xiaobing Ni Jie Luo Weidong Leng Weidong Leng |
| author_facet | Hedong Yu Hedong Yu Lingyun Xia Lingyun Xia Xieyuan Leng Yongji Chen Yongji Chen Li Zhang Li Zhang Xiaobing Ni Xiaobing Ni Jie Luo Weidong Leng Weidong Leng |
| author_sort | Hedong Yu |
| collection | DOAJ |
| description | Endothelial progenitor cells (EPCs) expressing vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) and bone marrow mesenchymal stem cells (BMSCs) expressing endogenous bone morphogenetic protein-2 (BMP-2) play the important role in new bone formation. This study investigated the effects of a porous hydroxyapatite (HA)/chitosan (CS)/polycaprolactone (PCL) composite scaffold-engrafted EPCs and BMSCs on the expression of BMP-2, VEGF, and PDGF in the calvarial defect rabbit model in vivo. It showed that a three-dimensional composite scaffold was successfully constructed by physical interaction with a pore size of 250 μm. The HA/CS/PCL scaffold degraded slowly within 10 weeks and showed non-cytotoxicity. By X-ray, micro-CT examination, and H&E staining, compared with the HA/CS/PCL group, HA/CS/PCL + EPCs, HA/CS/PCL + BMSCs, and HA/CS/PCL + EPCs + BMSCs groups performed a more obvious repair effect, and the dual factor group presented particularly significant improvement on the percentages of bone volume at week 4 and week 8, with evident bone growth. Osteogenesis marker (BMP-2) and vascularization marker (VEGF and PDGF) expression in the dual factor group were much better than those of the HA/CS/PCL control group and single factor groups. Collectively, the HA/CS/PCL composite scaffold-engrafting EPCs and BMSCs is effective to repair calvarial defects by regulating endogenous expression of BMP-2, VEGF, and PDGF. Thus, this study provides important implications for the potential clinical application of biomaterial composite scaffold-engrafted engineering cells. |
| first_indexed | 2024-12-11T18:49:57Z |
| format | Article |
| id | doaj.art-e28e1029fc6c4ce0b53d4991fb5057c0 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-11T18:49:57Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-e28e1029fc6c4ce0b53d4991fb5057c02022-12-22T00:54:20ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-08-011010.3389/fbioe.2022.928041928041Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCsHedong Yu0Hedong Yu1Lingyun Xia2Lingyun Xia3Xieyuan Leng4Yongji Chen5Yongji Chen6Li Zhang7Li Zhang8Xiaobing Ni9Xiaobing Ni10Jie Luo11Weidong Leng12Weidong Leng13Department of Stomatology, Taihe Hospital, Hubei University of Medicine, Shiyan, ChinaInstitute of Dental Research, School of Dentistry, Hubei University of Medicine, Shiyan, ChinaDepartment of Stomatology, Taihe Hospital, Hubei University of Medicine, Shiyan, ChinaInstitute of Dental Research, School of Dentistry, Hubei University of Medicine, Shiyan, ChinaThe First Clinical College, Anhui Medical University, Hefei, ChinaDepartment of Stomatology, Taihe Hospital, Hubei University of Medicine, Shiyan, ChinaInstitute of Dental Research, School of Dentistry, Hubei University of Medicine, Shiyan, ChinaDepartment of Stomatology, Taihe Hospital, Hubei University of Medicine, Shiyan, ChinaInstitute of Dental Research, School of Dentistry, Hubei University of Medicine, Shiyan, ChinaDepartment of Stomatology, Taihe Hospital, Hubei University of Medicine, Shiyan, ChinaInstitute of Dental Research, School of Dentistry, Hubei University of Medicine, Shiyan, ChinaDepartment of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, ChinaDepartment of Stomatology, Taihe Hospital, Hubei University of Medicine, Shiyan, ChinaInstitute of Dental Research, School of Dentistry, Hubei University of Medicine, Shiyan, ChinaEndothelial progenitor cells (EPCs) expressing vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) and bone marrow mesenchymal stem cells (BMSCs) expressing endogenous bone morphogenetic protein-2 (BMP-2) play the important role in new bone formation. This study investigated the effects of a porous hydroxyapatite (HA)/chitosan (CS)/polycaprolactone (PCL) composite scaffold-engrafted EPCs and BMSCs on the expression of BMP-2, VEGF, and PDGF in the calvarial defect rabbit model in vivo. It showed that a three-dimensional composite scaffold was successfully constructed by physical interaction with a pore size of 250 μm. The HA/CS/PCL scaffold degraded slowly within 10 weeks and showed non-cytotoxicity. By X-ray, micro-CT examination, and H&E staining, compared with the HA/CS/PCL group, HA/CS/PCL + EPCs, HA/CS/PCL + BMSCs, and HA/CS/PCL + EPCs + BMSCs groups performed a more obvious repair effect, and the dual factor group presented particularly significant improvement on the percentages of bone volume at week 4 and week 8, with evident bone growth. Osteogenesis marker (BMP-2) and vascularization marker (VEGF and PDGF) expression in the dual factor group were much better than those of the HA/CS/PCL control group and single factor groups. Collectively, the HA/CS/PCL composite scaffold-engrafting EPCs and BMSCs is effective to repair calvarial defects by regulating endogenous expression of BMP-2, VEGF, and PDGF. Thus, this study provides important implications for the potential clinical application of biomaterial composite scaffold-engrafted engineering cells.https://www.frontiersin.org/articles/10.3389/fbioe.2022.928041/fullHA/CS/PCL composite scaffoldendothelial progenitor cellsbone marrow mesenchymal stem cellscalvarial defectBMP-2VEGF |
| spellingShingle | Hedong Yu Hedong Yu Lingyun Xia Lingyun Xia Xieyuan Leng Yongji Chen Yongji Chen Li Zhang Li Zhang Xiaobing Ni Xiaobing Ni Jie Luo Weidong Leng Weidong Leng Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs Frontiers in Bioengineering and Biotechnology HA/CS/PCL composite scaffold endothelial progenitor cells bone marrow mesenchymal stem cells calvarial defect BMP-2 VEGF |
| title | Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs |
| title_full | Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs |
| title_fullStr | Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs |
| title_full_unstemmed | Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs |
| title_short | Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs |
| title_sort | improved repair of rabbit calvarial defects with hydroxyapatite chitosan polycaprolactone composite scaffold engrafted epcs and bmscs |
| topic | HA/CS/PCL composite scaffold endothelial progenitor cells bone marrow mesenchymal stem cells calvarial defect BMP-2 VEGF |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.928041/full |
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